Rapid and sensitive identification of epitope-containing peptides by direct matrix-assisted laser desorption/ionization tandem mass spectrometry of peptides affinity-bound to antibody beads

A method has been developed for rapid and sensitive identification of epitope-containing peptides, based on direct MALDI-MS/MS analysis of epitope-containing peptides affinity bound to affinity beads. This technique provides sequence information of the epitope that allows unambiguous identification...

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Veröffentlicht in:	Journal of the American Society for Mass Spectrometry 2003-10, Vol.14 (10), p.1076-1085
Hauptverfasser:	Raska, Christina S, Parker, Carol E, Sunnarborg, Susan W, Pope, R.Marshall, Lee, David C, Glish, Gary L, Borchers, Christoph H
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Amino Acid Sequence Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Antibodies Antibodies - immunology Antibodies, Phospho-Specific - immunology Antibody Specificity Avidin - metabolism Beads Biological and medical sciences Biotin - metabolism Coupling (molecular) Desorption Elution Epitopes - analysis Epitopes - chemistry Epitopes - immunology Fundamental and applied biological sciences. Psychology Ionization Ions Lasers Mass spectrometry Microspheres Peptides Peptides - analysis Peptides - chemistry Peptides - immunology Phosphotyrosine - immunology Protein Binding Proteins Proto-Oncogene Proteins c-myc - immunology Quadrupoles Searching Sensitivity analysis Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Time Factors
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