Optimized lentiviral vector production and purification procedure prevents immune response after transduction of mouse brain

HIV-derived lentiviral vectors are efficient vehicula to deliver genes into the brain and hold great promise for future gene therapy of neurodegenerative disorders. However, administration of the current vector preparations in mouse brain was found to induce a systemic immune response to vector prot...

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Veröffentlicht in:	Gene therapy 2003-11, Vol.10 (23), p.1933-1940
Hauptverfasser:	BAEKELANDT, V, EGGERMONT, K, MICHIELS, M, NUTTIN, B, DEBYSER, Z
Format:	Artikel
Sprache:	eng
Schlagworte:	Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animal models Animals Antibodies - blood Applied cell therapy and gene therapy Biological and medical sciences Biotechnology Brain - immunology Brain - metabolism Brain diseases Care and treatment Cell culture Culture Media, Serum-Free Female Fundamental and applied biological sciences. Psychology Gene Expression Gene therapy Genetic aspects Genetic Therapy - adverse effects Genetic Therapy - methods Genetic vectors Genetic Vectors - administration & dosage Genetic Vectors - immunology Green Fluorescent Proteins Health. Pharmaceutical industry HIV HIV-1 - genetics HIV-1 - immunology Human immunodeficiency virus Immune response Immunogenicity Industrial applications and implications. Economical aspects Inflammation Injections, Intraventricular Luminescent Proteins - genetics Male Medical sciences Methods Mice Mice, Inbred C57BL Neurodegenerative diseases Physiological aspects Sucrose Transduction Transduction, Genetic - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy Ultracentrifugation Virus Cultivation
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