Development of a serologic panel for the recognition of nocardial infections in a murine model
A murine model was used to develop a sensitive and specific serologic test for clinical and subclinical infections caused by Nocardia. The following tests were used: (a) enzyme-linked immunosorbent assays (ELISAs) with culture filtrate and cytoplasmic extract antigens from Nocardia asteroides; (b) E...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 1993-05, Vol.16 (4), p.291-301 |
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| creator | Kjelstrom, Judith A. Beaman, Blaine L. |
| description | A murine model was used to develop a sensitive and specific serologic test for clinical and subclinical infections caused by Nocardia. The following tests were used: (a) enzyme-linked immunosorbent assays (ELISAs) with culture filtrate and cytoplasmic extract antigens from
Nocardia asteroides; (b) ELISA with
N. asteroides trehalose dimycolate (cord factor); (c) indirect immunofluorescent antibody assay with whole cells of N. asteroides; and (d) Western-blot analysis for the 54 to 55-kD, 35-kD, and 62-kD proteins of
N. asteroides. The sera from BALB/c mice, experimentally infected with nonlethal doses of three species of
Nocardia, nine species of
Mycobacterium, Rhodococcus equi, two species of
Actinomadura, and two species of
Streptomyces were tested using this panel. The serologic tests did, indeed, identify mice infected with nocardiae and could differentiate them from mice infected with the other actinomycetes, including mycobacteria. |
| doi_str_mv | 10.1016/0732-8893(93)90079-M |
| format | Article |
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Nocardia asteroides; (b) ELISA with
N. asteroides trehalose dimycolate (cord factor); (c) indirect immunofluorescent antibody assay with whole cells of N. asteroides; and (d) Western-blot analysis for the 54 to 55-kD, 35-kD, and 62-kD proteins of
N. asteroides. The sera from BALB/c mice, experimentally infected with nonlethal doses of three species of
Nocardia, nine species of
Mycobacterium, Rhodococcus equi, two species of
Actinomadura, and two species of
Streptomyces were tested using this panel. The serologic tests did, indeed, identify mice infected with nocardiae and could differentiate them from mice infected with the other actinomycetes, including mycobacteria.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/0732-8893(93)90079-M</identifier><identifier>PMID: 8495584</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Actinomycetales - immunology ; Animals ; Antibodies, Bacterial - blood ; Antibodies, Bacterial - immunology ; Antigens, Bacterial - immunology ; Bacterial diseases ; Biological and medical sciences ; Blotting, Western ; Cell Line ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Experimental bacterial diseases and models ; Female ; Fluorescent Antibody Technique ; Horses ; Humans ; Infectious diseases ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Nocardia asteroides - immunology ; Nocardia Infections - blood ; Nocardia Infections - diagnosis ; Serologic Tests - methods</subject><ispartof>Diagnostic microbiology and infectious disease, 1993-05, Vol.16 (4), p.291-301</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-1774364575a75aa1862b8ef10ce7c67380677a745e3235169caa22cfdd69d80c3</citedby><cites>FETCH-LOGICAL-c386t-1774364575a75aa1862b8ef10ce7c67380677a745e3235169caa22cfdd69d80c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/073288939390079M$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4760464$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8495584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kjelstrom, Judith A.</creatorcontrib><creatorcontrib>Beaman, Blaine L.</creatorcontrib><title>Development of a serologic panel for the recognition of nocardial infections in a murine model</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>A murine model was used to develop a sensitive and specific serologic test for clinical and subclinical infections caused by Nocardia. The following tests were used: (a) enzyme-linked immunosorbent assays (ELISAs) with culture filtrate and cytoplasmic extract antigens from
Nocardia asteroides; (b) ELISA with
N. asteroides trehalose dimycolate (cord factor); (c) indirect immunofluorescent antibody assay with whole cells of N. asteroides; and (d) Western-blot analysis for the 54 to 55-kD, 35-kD, and 62-kD proteins of
N. asteroides. The sera from BALB/c mice, experimentally infected with nonlethal doses of three species of
Nocardia, nine species of
Mycobacterium, Rhodococcus equi, two species of
Actinomadura, and two species of
Streptomyces were tested using this panel. The serologic tests did, indeed, identify mice infected with nocardiae and could differentiate them from mice infected with the other actinomycetes, including mycobacteria.</description><subject>Actinomycetales - immunology</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Disease Models, Animal</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Experimental bacterial diseases and models</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Horses</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nocardia asteroides - immunology</subject><subject>Nocardia Infections - blood</subject><subject>Nocardia Infections - diagnosis</subject><subject>Serologic Tests - methods</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kN9LHDEQgENR7Gn7H7SQByn2YW2yyebHi1C0VeHEF3015LKz15Rsck32BP97s9xxj4WBzGS-GZIPoS-UXFJCxQ8iWdsopdmFZt81IVI3Dx_Qgqqa1IococUB-YhOS_lLCG01JyfoRHHddYov0MsNvEJImxHihNOALS6QU0hr7_DGRgh4SBlPfwBncGkd_eRTnMGYnM29twH7OICbr0tN64Jxm30EPKYewid0PNhQ4PP-PEPPv389Xd81y8fb--ufy8YxJaaGSsmZ4J3sbA1LlWhXCgZKHEgnJFNESGkl74C1rKNCO2vb1g19L3SviGNn6Ntu7yanf1sokxl9cRBC_ULaFiM7yUWnRQX5DnQ5lZJhMJvsR5vfDCVm1mpmZ2Z2ZuaYtZqHOvZ1v3-7GqE_DO091v75vm-Ls2HINjpfDhiXgnAxY1c7DKqLVw_ZFOchOuh91TuZPvn_v-MdIFKTpw</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>Kjelstrom, Judith A.</creator><creator>Beaman, Blaine L.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930501</creationdate><title>Development of a serologic panel for the recognition of nocardial infections in a murine model</title><author>Kjelstrom, Judith A. ; Beaman, Blaine L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-1774364575a75aa1862b8ef10ce7c67380677a745e3235169caa22cfdd69d80c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Actinomycetales - immunology</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>Disease Models, Animal</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Experimental bacterial diseases and models</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Horses</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nocardia asteroides - immunology</topic><topic>Nocardia Infections - blood</topic><topic>Nocardia Infections - diagnosis</topic><topic>Serologic Tests - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kjelstrom, Judith A.</creatorcontrib><creatorcontrib>Beaman, Blaine L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kjelstrom, Judith A.</au><au>Beaman, Blaine L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a serologic panel for the recognition of nocardial infections in a murine model</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>1993-05-01</date><risdate>1993</risdate><volume>16</volume><issue>4</issue><spage>291</spage><epage>301</epage><pages>291-301</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>A murine model was used to develop a sensitive and specific serologic test for clinical and subclinical infections caused by Nocardia. The following tests were used: (a) enzyme-linked immunosorbent assays (ELISAs) with culture filtrate and cytoplasmic extract antigens from
Nocardia asteroides; (b) ELISA with
N. asteroides trehalose dimycolate (cord factor); (c) indirect immunofluorescent antibody assay with whole cells of N. asteroides; and (d) Western-blot analysis for the 54 to 55-kD, 35-kD, and 62-kD proteins of
N. asteroides. The sera from BALB/c mice, experimentally infected with nonlethal doses of three species of
Nocardia, nine species of
Mycobacterium, Rhodococcus equi, two species of
Actinomadura, and two species of
Streptomyces were tested using this panel. The serologic tests did, indeed, identify mice infected with nocardiae and could differentiate them from mice infected with the other actinomycetes, including mycobacteria.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>8495584</pmid><doi>10.1016/0732-8893(93)90079-M</doi><tpages>11</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 1993-05, Vol.16 (4), p.291-301 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Actinomycetales - immunology Animals Antibodies, Bacterial - blood Antibodies, Bacterial - immunology Antigens, Bacterial - immunology Bacterial diseases Biological and medical sciences Blotting, Western Cell Line Disease Models, Animal Enzyme-Linked Immunosorbent Assay Experimental bacterial diseases and models Female Fluorescent Antibody Technique Horses Humans Infectious diseases Medical sciences Mice Mice, Inbred BALB C Nocardia asteroides - immunology Nocardia Infections - blood Nocardia Infections - diagnosis Serologic Tests - methods |
| title | Development of a serologic panel for the recognition of nocardial infections in a murine model |
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