A new model of implant-related osteomyelitis in rats
Infection related to osteosynthesis often has dramatic consequences for the patient. Prolonged hospitalization with systemic antibiotic therapy, several revision procedures, possible amputation, and even death may occur. To investigate the pathology of infection in orthopedic surgery, a new rat mode...
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| Veröffentlicht in: | Journal of biomedical materials research 2003-10, Vol.67B (1), p.593-602 |
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| creator | Lucke, M. Schmidmaier, G. Sadoni, S. Wildemann, B. Schiller, R. Stemberger, A. Haas, N.P. Raschke, M. |
| description | Infection related to osteosynthesis often has dramatic consequences for the patient. Prolonged hospitalization with systemic antibiotic therapy, several revision procedures, possible amputation, and even death may occur. To investigate the pathology of infection in orthopedic surgery, a new rat model of implant related osteomyelitis was developed. Three different concentrations (106, 103, and 102 colony‐forming units (CFU)/10 μl) of Staphylococcus aureus were inoculated into the tibial medullary cavity with simultaneous insertion of a titanium Kirschner wire. Controls received phosphate‐buffered saline (PBS). Each group consisted of 10 animals. Animals were followed for 4 weeks until sacrifice. X‐rays of the tibiae were taken weekly, blood counts were analyzed, and body temperature and weight were determined. After sacrifice, infection was evaluated by histological and microbiological investigations. All animals inoculated with Staph. aureus in either concentration developed microbiological, histological, and radiological signs of osteomyelitis in correlation to the amount of inoculated bacteria. X‐rays clearly revealed osseous destruction after 14 days with progression of osteomyelitis during the following weeks. CFU/g bone and bone weight after sacrifice showed dependence on the amount of inoculated CFU. The histological results confirmed the radiological findings. No significant changes in blood counts, body weight, and body temperature between the groups could be observed. The results demonstrate that it is possible to develop a model of implant‐related osteomyelitis in rats with dependence on the amount of inoculated bacteria. No other promoters of infection besides intramedullary insertion of titanium Kirschner wires were used in this model. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 67B: 593–602, 2003 |
| doi_str_mv | 10.1002/jbm.b.10051 |
| format | Article |
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Prolonged hospitalization with systemic antibiotic therapy, several revision procedures, possible amputation, and even death may occur. To investigate the pathology of infection in orthopedic surgery, a new rat model of implant related osteomyelitis was developed. Three different concentrations (106, 103, and 102 colony‐forming units (CFU)/10 μl) of Staphylococcus aureus were inoculated into the tibial medullary cavity with simultaneous insertion of a titanium Kirschner wire. Controls received phosphate‐buffered saline (PBS). Each group consisted of 10 animals. Animals were followed for 4 weeks until sacrifice. X‐rays of the tibiae were taken weekly, blood counts were analyzed, and body temperature and weight were determined. After sacrifice, infection was evaluated by histological and microbiological investigations. All animals inoculated with Staph. aureus in either concentration developed microbiological, histological, and radiological signs of osteomyelitis in correlation to the amount of inoculated bacteria. X‐rays clearly revealed osseous destruction after 14 days with progression of osteomyelitis during the following weeks. CFU/g bone and bone weight after sacrifice showed dependence on the amount of inoculated CFU. The histological results confirmed the radiological findings. No significant changes in blood counts, body weight, and body temperature between the groups could be observed. The results demonstrate that it is possible to develop a model of implant‐related osteomyelitis in rats with dependence on the amount of inoculated bacteria. No other promoters of infection besides intramedullary insertion of titanium Kirschner wires were used in this model. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 67B: 593–602, 2003</description><identifier>ISSN: 1552-4973</identifier><identifier>ISSN: 0021-9304</identifier><identifier>EISSN: 1552-4981</identifier><identifier>EISSN: 1097-4636</identifier><identifier>DOI: 10.1002/jbm.b.10051</identifier><identifier>PMID: 14528456</identifier><identifier>CODEN: JBMRBG</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Blood - microbiology ; Blood Chemical Analysis ; Body Temperature ; Body Weight ; Bone Wires - adverse effects ; Colony Count, Microbial ; Disease Models, Animal ; Female ; Humans ; implant-related infection ; Medical sciences ; osteomyelitis ; Osteomyelitis - etiology ; Osteomyelitis - physiopathology ; Prostheses and Implants - adverse effects ; Prosthesis-Related Infections - physiopathology ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; rat model ; Rats ; Rats, Sprague-Dawley ; Staphylococcal Infections - physiopathology ; Staphylococcus aureus ; Technology. Biomaterials. Equipments. Material. Instrumentation ; Tibia - microbiology ; Tibia - pathology ; Titanium</subject><ispartof>Journal of biomedical materials research, 2003-10, Vol.67B (1), p.593-602</ispartof><rights>Copyright © 2003 Wiley Periodicals, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2003 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5531-819d78bc0fcf7b13d21130c9c7360d17b52526e0e7f658959025039d9ddab8373</citedby><cites>FETCH-LOGICAL-c5531-819d78bc0fcf7b13d21130c9c7360d17b52526e0e7f658959025039d9ddab8373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.b.10051$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.b.10051$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15177877$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15433120$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14528456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lucke, M.</creatorcontrib><creatorcontrib>Schmidmaier, G.</creatorcontrib><creatorcontrib>Sadoni, S.</creatorcontrib><creatorcontrib>Wildemann, B.</creatorcontrib><creatorcontrib>Schiller, R.</creatorcontrib><creatorcontrib>Stemberger, A.</creatorcontrib><creatorcontrib>Haas, N.P.</creatorcontrib><creatorcontrib>Raschke, M.</creatorcontrib><title>A new model of implant-related osteomyelitis in rats</title><title>Journal of biomedical materials research</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Infection related to osteosynthesis often has dramatic consequences for the patient. Prolonged hospitalization with systemic antibiotic therapy, several revision procedures, possible amputation, and even death may occur. To investigate the pathology of infection in orthopedic surgery, a new rat model of implant related osteomyelitis was developed. Three different concentrations (106, 103, and 102 colony‐forming units (CFU)/10 μl) of Staphylococcus aureus were inoculated into the tibial medullary cavity with simultaneous insertion of a titanium Kirschner wire. Controls received phosphate‐buffered saline (PBS). Each group consisted of 10 animals. Animals were followed for 4 weeks until sacrifice. X‐rays of the tibiae were taken weekly, blood counts were analyzed, and body temperature and weight were determined. After sacrifice, infection was evaluated by histological and microbiological investigations. All animals inoculated with Staph. aureus in either concentration developed microbiological, histological, and radiological signs of osteomyelitis in correlation to the amount of inoculated bacteria. X‐rays clearly revealed osseous destruction after 14 days with progression of osteomyelitis during the following weeks. CFU/g bone and bone weight after sacrifice showed dependence on the amount of inoculated CFU. The histological results confirmed the radiological findings. No significant changes in blood counts, body weight, and body temperature between the groups could be observed. The results demonstrate that it is possible to develop a model of implant‐related osteomyelitis in rats with dependence on the amount of inoculated bacteria. No other promoters of infection besides intramedullary insertion of titanium Kirschner wires were used in this model. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 67B: 593–602, 2003</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood - microbiology</subject><subject>Blood Chemical Analysis</subject><subject>Body Temperature</subject><subject>Body Weight</subject><subject>Bone Wires - adverse effects</subject><subject>Colony Count, Microbial</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Humans</subject><subject>implant-related infection</subject><subject>Medical sciences</subject><subject>osteomyelitis</subject><subject>Osteomyelitis - etiology</subject><subject>Osteomyelitis - physiopathology</subject><subject>Prostheses and Implants - adverse effects</subject><subject>Prosthesis-Related Infections - physiopathology</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>rat model</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Staphylococcal Infections - physiopathology</subject><subject>Staphylococcus aureus</subject><subject>Technology. Biomaterials. Equipments. Material. Instrumentation</subject><subject>Tibia - microbiology</subject><subject>Tibia - pathology</subject><subject>Titanium</subject><issn>1552-4973</issn><issn>0021-9304</issn><issn>1552-4981</issn><issn>1097-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0T1PHDEQBmArCgofSUWPtkkatOCxd9Z2SS6EDwFpQKSzvLZXMtm9Pew9Xe7f48sd0OUqT_HM6NVrQg6BngCl7PSp6U-a1YjwgewBIisrJeHj2yz4LtlP6SnjmiL_RHahQiYrrPdIdVZM_aLoB-e7YmiL0M86Mx3L6DszelcMafRDv_RdGEMqwrSIZkyfyU5ruuS_bN4D8vDz_H5yWd78urianN2UFpFDKUE5IRtLW9uKBrhjAJxaZQWvqQPRIENWe-pFW6NUqChDypVTzplGcsEPyLf13Vkcnuc-jboPyfouJ_TDPGmBomJKsq2QCcWhxmo7pIgyn83weA1tHFKKvtWzGHoTlxqoXtWuc-260f9qz_poc3be9N69203PGXzdAJOs6dpopjakd5ejcWB0uwMhpFjFg7VbhM4v_5dNX3-_fQ1ZrndC_tO_bzsm_tG14AL1492Fht-Tyx_3t0pP-AtIYrMy</recordid><startdate>20031015</startdate><enddate>20031015</enddate><creator>Lucke, M.</creator><creator>Schmidmaier, G.</creator><creator>Sadoni, S.</creator><creator>Wildemann, B.</creator><creator>Schiller, R.</creator><creator>Stemberger, A.</creator><creator>Haas, N.P.</creator><creator>Raschke, M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>John Wiley & Sons</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>8BQ</scope><scope>JG9</scope><scope>7X8</scope></search><sort><creationdate>20031015</creationdate><title>A new model of implant-related osteomyelitis in rats</title><author>Lucke, M. ; Schmidmaier, G. ; Sadoni, S. ; Wildemann, B. ; Schiller, R. ; Stemberger, A. ; Haas, N.P. ; Raschke, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5531-819d78bc0fcf7b13d21130c9c7360d17b52526e0e7f658959025039d9ddab8373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood - microbiology</topic><topic>Blood Chemical Analysis</topic><topic>Body Temperature</topic><topic>Body Weight</topic><topic>Bone Wires - adverse effects</topic><topic>Colony Count, Microbial</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Humans</topic><topic>implant-related infection</topic><topic>Medical sciences</topic><topic>osteomyelitis</topic><topic>Osteomyelitis - etiology</topic><topic>Osteomyelitis - physiopathology</topic><topic>Prostheses and Implants - adverse effects</topic><topic>Prosthesis-Related Infections - physiopathology</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>rat model</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Staphylococcal Infections - physiopathology</topic><topic>Staphylococcus aureus</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><topic>Tibia - microbiology</topic><topic>Tibia - pathology</topic><topic>Titanium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lucke, M.</creatorcontrib><creatorcontrib>Schmidmaier, G.</creatorcontrib><creatorcontrib>Sadoni, S.</creatorcontrib><creatorcontrib>Wildemann, B.</creatorcontrib><creatorcontrib>Schiller, R.</creatorcontrib><creatorcontrib>Stemberger, A.</creatorcontrib><creatorcontrib>Haas, N.P.</creatorcontrib><creatorcontrib>Raschke, M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lucke, M.</au><au>Schmidmaier, G.</au><au>Sadoni, S.</au><au>Wildemann, B.</au><au>Schiller, R.</au><au>Stemberger, A.</au><au>Haas, N.P.</au><au>Raschke, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new model of implant-related osteomyelitis in rats</atitle><jtitle>Journal of biomedical materials research</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2003-10-15</date><risdate>2003</risdate><volume>67B</volume><issue>1</issue><spage>593</spage><epage>602</epage><pages>593-602</pages><issn>1552-4973</issn><issn>0021-9304</issn><eissn>1552-4981</eissn><eissn>1097-4636</eissn><coden>JBMRBG</coden><abstract>Infection related to osteosynthesis often has dramatic consequences for the patient. Prolonged hospitalization with systemic antibiotic therapy, several revision procedures, possible amputation, and even death may occur. To investigate the pathology of infection in orthopedic surgery, a new rat model of implant related osteomyelitis was developed. Three different concentrations (106, 103, and 102 colony‐forming units (CFU)/10 μl) of Staphylococcus aureus were inoculated into the tibial medullary cavity with simultaneous insertion of a titanium Kirschner wire. Controls received phosphate‐buffered saline (PBS). Each group consisted of 10 animals. Animals were followed for 4 weeks until sacrifice. X‐rays of the tibiae were taken weekly, blood counts were analyzed, and body temperature and weight were determined. After sacrifice, infection was evaluated by histological and microbiological investigations. All animals inoculated with Staph. aureus in either concentration developed microbiological, histological, and radiological signs of osteomyelitis in correlation to the amount of inoculated bacteria. X‐rays clearly revealed osseous destruction after 14 days with progression of osteomyelitis during the following weeks. CFU/g bone and bone weight after sacrifice showed dependence on the amount of inoculated CFU. The histological results confirmed the radiological findings. No significant changes in blood counts, body weight, and body temperature between the groups could be observed. The results demonstrate that it is possible to develop a model of implant‐related osteomyelitis in rats with dependence on the amount of inoculated bacteria. No other promoters of infection besides intramedullary insertion of titanium Kirschner wires were used in this model. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 67B: 593–602, 2003</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>14528456</pmid><doi>10.1002/jbm.b.10051</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Blood - microbiology Blood Chemical Analysis Body Temperature Body Weight Bone Wires - adverse effects Colony Count, Microbial Disease Models, Animal Female Humans implant-related infection Medical sciences osteomyelitis Osteomyelitis - etiology Osteomyelitis - physiopathology Prostheses and Implants - adverse effects Prosthesis-Related Infections - physiopathology Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) rat model Rats Rats, Sprague-Dawley Staphylococcal Infections - physiopathology Staphylococcus aureus Technology. Biomaterials. Equipments. Material. Instrumentation Tibia - microbiology Tibia - pathology Titanium |
| title | A new model of implant-related osteomyelitis in rats |
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