Organisation of the tomato polyphenol oxidase gene family
We report the isolation and characterization of seven nuclear genes encoding polyphenol oxidase (PPO) in tomato (Lycopersicon esculentum cv. VFNT Cherry). The seven genes (PPOs A, A', B, C, D, E and F) fall into three structural classes (I, II, and III) based on Eco RI and Hind III restriction...
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| Veröffentlicht in: | Plant molecular biology 1993-03, Vol.21 (6), p.1035-1051 |
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| container_title | Plant molecular biology |
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| creator | NEWMAN, S. M EANNETTA, N. T HAIFENG YU PRINCE, J. P DE VICNETE, M. C TANKSLEY, S. D STEFFENS, J. C |
| description | We report the isolation and characterization of seven nuclear genes encoding polyphenol oxidase (PPO) in tomato (Lycopersicon esculentum cv. VFNT Cherry). The seven genes (PPOs A, A', B, C, D, E and F) fall into three structural classes (I, II, and III) based on Eco RI and Hind III restriction fragment length polymorphisms (RFLP). RFLP mapping and PFGE analysis demonstrated that the genes reside on chromosome 8, and may be clustered within a 165 kb region. Phage insert mapping demonstrated PPO E and PPO F (both class III), and PPOs B, D and A (classes I, II and I respectively) are grouped within separate 12.4 kb clusters. The complete nucleotide sequence was determined for each gene. Comparison to cDNAs revealed that the PPOs lack introns. A transcript of about 2 kb is expected for each PPO. Each PPO possesses a region encoding a transit peptide characteristic of polypeptides targeted to the thylakoid lumen. Predicted precursor polypeptides range in mass from 66 to 71 kDa and predicted mature polypeptides range from 57 to 62 kDa. All the PPOs encode two putative copper-binding sites characteristic of bacterial, fungal and mammalian tyrosinases. Five of the seven PPOs possess divergent DNA sequences in their 5' promoter regions. These flanking sequence differences may regulate the differential expression of PPO genes. |
| doi_str_mv | 10.1007/BF00023601 |
| format | Article |
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M ; EANNETTA, N. T ; HAIFENG YU ; PRINCE, J. P ; DE VICNETE, M. C ; TANKSLEY, S. D ; STEFFENS, J. C</creator><creatorcontrib>NEWMAN, S. M ; EANNETTA, N. T ; HAIFENG YU ; PRINCE, J. P ; DE VICNETE, M. C ; TANKSLEY, S. D ; STEFFENS, J. C ; Cornell Univ., Ithaca, NY (USA). Dept. of Plant Breeding and Biometry ; Universidad Catolica de Chile, Santiago (Chile). Fac. de Agronomia</creatorcontrib><description>We report the isolation and characterization of seven nuclear genes encoding polyphenol oxidase (PPO) in tomato (Lycopersicon esculentum cv. VFNT Cherry). The seven genes (PPOs A, A', B, C, D, E and F) fall into three structural classes (I, II, and III) based on Eco RI and Hind III restriction fragment length polymorphisms (RFLP). RFLP mapping and PFGE analysis demonstrated that the genes reside on chromosome 8, and may be clustered within a 165 kb region. Phage insert mapping demonstrated PPO E and PPO F (both class III), and PPOs B, D and A (classes I, II and I respectively) are grouped within separate 12.4 kb clusters. The complete nucleotide sequence was determined for each gene. Comparison to cDNAs revealed that the PPOs lack introns. A transcript of about 2 kb is expected for each PPO. Each PPO possesses a region encoding a transit peptide characteristic of polypeptides targeted to the thylakoid lumen. Predicted precursor polypeptides range in mass from 66 to 71 kDa and predicted mature polypeptides range from 57 to 62 kDa. All the PPOs encode two putative copper-binding sites characteristic of bacterial, fungal and mammalian tyrosinases. Five of the seven PPOs possess divergent DNA sequences in their 5' promoter regions. These flanking sequence differences may regulate the differential expression of PPO genes.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00023601</identifier><identifier>PMID: 8098228</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Amino Acid Sequence ; Biological and medical sciences ; Blotting, Southern ; Catechol Oxidase - genetics ; Catechol Oxidase - metabolism ; Cloning, Molecular ; code genetique ; codigo genetico ; compose phenolique ; compuestos fenolicos ; DNA ; Fundamental and applied biological sciences. Psychology ; gene ; genes ; Genes, Plant ; Genes. Genome ; genetic code ; Lycopersicon esculentum ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Multigene Family ; nucleotide sequence ; peptide ; peptides ; peptidos ; phenolic compounds ; Plants - enzymology ; Plants - genetics ; Polymorphism, Restriction Fragment Length ; Restriction Mapping ; secuencia nucleica ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; sequence nucleique</subject><ispartof>Plant molecular biology, 1993-03, Vol.21 (6), p.1035-1051</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-f5df7f1a4760311c6c0ddfc57e5f22d81a9f74f74bac828f727cb1ab78733753</citedby><cites>FETCH-LOGICAL-c406t-f5df7f1a4760311c6c0ddfc57e5f22d81a9f74f74bac828f727cb1ab78733753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4734677$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8098228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NEWMAN, S. M</creatorcontrib><creatorcontrib>EANNETTA, N. T</creatorcontrib><creatorcontrib>HAIFENG YU</creatorcontrib><creatorcontrib>PRINCE, J. P</creatorcontrib><creatorcontrib>DE VICNETE, M. C</creatorcontrib><creatorcontrib>TANKSLEY, S. D</creatorcontrib><creatorcontrib>STEFFENS, J. C</creatorcontrib><creatorcontrib>Cornell Univ., Ithaca, NY (USA). Dept. of Plant Breeding and Biometry</creatorcontrib><creatorcontrib>Universidad Catolica de Chile, Santiago (Chile). Fac. de Agronomia</creatorcontrib><title>Organisation of the tomato polyphenol oxidase gene family</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>We report the isolation and characterization of seven nuclear genes encoding polyphenol oxidase (PPO) in tomato (Lycopersicon esculentum cv. VFNT Cherry). The seven genes (PPOs A, A', B, C, D, E and F) fall into three structural classes (I, II, and III) based on Eco RI and Hind III restriction fragment length polymorphisms (RFLP). RFLP mapping and PFGE analysis demonstrated that the genes reside on chromosome 8, and may be clustered within a 165 kb region. Phage insert mapping demonstrated PPO E and PPO F (both class III), and PPOs B, D and A (classes I, II and I respectively) are grouped within separate 12.4 kb clusters. The complete nucleotide sequence was determined for each gene. Comparison to cDNAs revealed that the PPOs lack introns. A transcript of about 2 kb is expected for each PPO. Each PPO possesses a region encoding a transit peptide characteristic of polypeptides targeted to the thylakoid lumen. Predicted precursor polypeptides range in mass from 66 to 71 kDa and predicted mature polypeptides range from 57 to 62 kDa. All the PPOs encode two putative copper-binding sites characteristic of bacterial, fungal and mammalian tyrosinases. Five of the seven PPOs possess divergent DNA sequences in their 5' promoter regions. These flanking sequence differences may regulate the differential expression of PPO genes.</description><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Catechol Oxidase - genetics</subject><subject>Catechol Oxidase - metabolism</subject><subject>Cloning, Molecular</subject><subject>code genetique</subject><subject>codigo genetico</subject><subject>compose phenolique</subject><subject>compuestos fenolicos</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>genetic code</subject><subject>Lycopersicon esculentum</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>nucleotide sequence</subject><subject>peptide</subject><subject>peptides</subject><subject>peptidos</subject><subject>phenolic compounds</subject><subject>Plants - enzymology</subject><subject>Plants - genetics</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Restriction Mapping</subject><subject>secuencia nucleica</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>sequence nucleique</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1Lw0AQBuBFlFo_Lt6VHMSDEJ39nOSoxapQ9OI9TDe7bSTJ1mwK9t8baalHYYc9zMMM8zJ2weGOA-D94xQAhDTAD9iYa5SpBpEdsjFwg6lSXByzkxg_AQYuzYiNMsgzIbIxy9-7BbVVpL4KbRJ80i9d0oeG-pCsQr1ZLV0b6iR8VyVFlyxc6xJPTVVvztiRpzq6891_yj6mTx-Tl3T2_vw6eZilVoHpU69Lj56TQgOSc2sslKW3Gp32QpQZp9yjGt6cbCYyjwLtnNMcM5QStTxlN9uxqy58rV3si6aK1tU1tS6sY4HDuTqX-C_kRqMaaoC3W2i7EGPnfLHqqoa6TcGh-M2z-MtzwJe7qet548o93QU49K93fYqWat9Ra6u4ZwqlMvi782rLPIWCFt1A3mY8zyWAUToX8gcr4oRB</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>NEWMAN, S. M</creator><creator>EANNETTA, N. T</creator><creator>HAIFENG YU</creator><creator>PRINCE, J. P</creator><creator>DE VICNETE, M. C</creator><creator>TANKSLEY, S. D</creator><creator>STEFFENS, J. C</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>Organisation of the tomato polyphenol oxidase gene family</title><author>NEWMAN, S. M ; EANNETTA, N. T ; HAIFENG YU ; PRINCE, J. P ; DE VICNETE, M. C ; TANKSLEY, S. D ; STEFFENS, J. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-f5df7f1a4760311c6c0ddfc57e5f22d81a9f74f74bac828f727cb1ab78733753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Catechol Oxidase - genetics</topic><topic>Catechol Oxidase - metabolism</topic><topic>Cloning, Molecular</topic><topic>code genetique</topic><topic>codigo genetico</topic><topic>compose phenolique</topic><topic>compuestos fenolicos</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>genetic code</topic><topic>Lycopersicon esculentum</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>nucleotide sequence</topic><topic>peptide</topic><topic>peptides</topic><topic>peptidos</topic><topic>phenolic compounds</topic><topic>Plants - enzymology</topic><topic>Plants - genetics</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Restriction Mapping</topic><topic>secuencia nucleica</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>sequence nucleique</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NEWMAN, S. M</creatorcontrib><creatorcontrib>EANNETTA, N. T</creatorcontrib><creatorcontrib>HAIFENG YU</creatorcontrib><creatorcontrib>PRINCE, J. P</creatorcontrib><creatorcontrib>DE VICNETE, M. C</creatorcontrib><creatorcontrib>TANKSLEY, S. D</creatorcontrib><creatorcontrib>STEFFENS, J. C</creatorcontrib><creatorcontrib>Cornell Univ., Ithaca, NY (USA). Dept. of Plant Breeding and Biometry</creatorcontrib><creatorcontrib>Universidad Catolica de Chile, Santiago (Chile). Fac. de Agronomia</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NEWMAN, S. M</au><au>EANNETTA, N. T</au><au>HAIFENG YU</au><au>PRINCE, J. P</au><au>DE VICNETE, M. C</au><au>TANKSLEY, S. D</au><au>STEFFENS, J. C</au><aucorp>Cornell Univ., Ithaca, NY (USA). Dept. of Plant Breeding and Biometry</aucorp><aucorp>Universidad Catolica de Chile, Santiago (Chile). Fac. de Agronomia</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organisation of the tomato polyphenol oxidase gene family</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>21</volume><issue>6</issue><spage>1035</spage><epage>1051</epage><pages>1035-1051</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>We report the isolation and characterization of seven nuclear genes encoding polyphenol oxidase (PPO) in tomato (Lycopersicon esculentum cv. VFNT Cherry). The seven genes (PPOs A, A', B, C, D, E and F) fall into three structural classes (I, II, and III) based on Eco RI and Hind III restriction fragment length polymorphisms (RFLP). RFLP mapping and PFGE analysis demonstrated that the genes reside on chromosome 8, and may be clustered within a 165 kb region. Phage insert mapping demonstrated PPO E and PPO F (both class III), and PPOs B, D and A (classes I, II and I respectively) are grouped within separate 12.4 kb clusters. The complete nucleotide sequence was determined for each gene. Comparison to cDNAs revealed that the PPOs lack introns. A transcript of about 2 kb is expected for each PPO. Each PPO possesses a region encoding a transit peptide characteristic of polypeptides targeted to the thylakoid lumen. Predicted precursor polypeptides range in mass from 66 to 71 kDa and predicted mature polypeptides range from 57 to 62 kDa. All the PPOs encode two putative copper-binding sites characteristic of bacterial, fungal and mammalian tyrosinases. Five of the seven PPOs possess divergent DNA sequences in their 5' promoter regions. These flanking sequence differences may regulate the differential expression of PPO genes.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>8098228</pmid><doi>10.1007/BF00023601</doi><tpages>17</tpages></addata></record> |
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| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1993-03, Vol.21 (6), p.1035-1051 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amino Acid Sequence Biological and medical sciences Blotting, Southern Catechol Oxidase - genetics Catechol Oxidase - metabolism Cloning, Molecular code genetique codigo genetico compose phenolique compuestos fenolicos DNA Fundamental and applied biological sciences. Psychology gene genes Genes, Plant Genes. Genome genetic code Lycopersicon esculentum Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family nucleotide sequence peptide peptides peptidos phenolic compounds Plants - enzymology Plants - genetics Polymorphism, Restriction Fragment Length Restriction Mapping secuencia nucleica Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid sequence nucleique |
| title | Organisation of the tomato polyphenol oxidase gene family |
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