Interaction between gfp-tagged Pseudomonas tolaasii P12 and Pleurotus eryngii

Pseudomonas sp., (formerly reported as strain P12) which produces brown blotch disease symptoms on Pleurotus eryngii, has been identified as P. tolaasii based on its biochemical, physiological properties and 16S rDNA sequence analysis. This pathogen is able to infect basidiocarps when surface-inocul...

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Veröffentlicht in:Microbiological research 2003-01, Vol.158 (3), p.265-270
Hauptverfasser: Russo, Anna, Filippi, Carlo, Tombolini, Riccardo, Toffanin, Annita, Bedini, Stefano, Agnolucci, Monica, Nuti, Marco
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container_end_page 270
container_issue 3
container_start_page 265
container_title Microbiological research
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creator Russo, Anna
Filippi, Carlo
Tombolini, Riccardo
Toffanin, Annita
Bedini, Stefano
Agnolucci, Monica
Nuti, Marco
description Pseudomonas sp., (formerly reported as strain P12) which produces brown blotch disease symptoms on Pleurotus eryngii, has been identified as P. tolaasii based on its biochemical, physiological properties and 16S rDNA sequence analysis. This pathogen is able to infect basidiocarps when surface-inoculated on mushroom casing soil. However, infected basidiocarps develop the brown blotch disease symptoms when the pathogen concentration in the fruiting body tissues is higher than 10 4 cfu/g d.w. Using gfp-tagged cells and confocal laser scanning microscopy, it was possible to show that the pathogen has the ability to tightly attach to the hyphae of Pleurotus eryngii.
doi_str_mv 10.1078/0944-5013-00203
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This pathogen is able to infect basidiocarps when surface-inoculated on mushroom casing soil. However, infected basidiocarps develop the brown blotch disease symptoms when the pathogen concentration in the fruiting body tissues is higher than 10 4 cfu/g d.w. Using gfp-tagged cells and confocal laser scanning microscopy, it was possible to show that the pathogen has the ability to tightly attach to the hyphae of Pleurotus eryngii.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1078/0944-5013-00203</identifier><identifier>PMID: 14521237</identifier><language>eng</language><publisher>Basingstoke: Elsevier GmbH</publisher><subject>Bacterial Adhesion ; Bacteriology ; Biological and medical sciences ; brown blotch disease ; DNA Transposable Elements ; DNA, Recombinant ; DNA, Ribosomal - chemistry ; Fundamental and applied biological sciences. 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subjects Bacterial Adhesion
Bacteriology
Biological and medical sciences
brown blotch disease
DNA Transposable Elements
DNA, Recombinant
DNA, Ribosomal - chemistry
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Genome, Bacterial
gfp
Green Fluorescent Proteins
Hyphae - physiology
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Microbiology
Microscopy, Fluorescence
Molecular Sequence Data
Mycelium - physiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pleurotus - cytology
Pleurotus - physiology
Pleurotus eryngii
Pseudomonas - classification
Pseudomonas - genetics
Pseudomonas - physiology
Pseudomonas tolaasii
Staining and Labeling
title Interaction between gfp-tagged Pseudomonas tolaasii P12 and Pleurotus eryngii
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