Early detection of apoptosis by staining of acid‐treated apoptotic cells with FITC‐labeled lectin from Narcissus pseudonarcissus

Background Exposure of anionic phospholipids and modified carbohydrates are main parts of the apoptotic death program. Cells undergoing apoptosis can be identified by various methods, detecting surface changes or modifications of their organelles, respectively. We describe a method for the detection...

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Veröffentlicht in:	Cytometry. Part A 2003-10, Vol.55A (2), p.86-93
Hauptverfasser:	Heyder, Petra, Gaipl, Udo S., Beyer, Thomas D., Voll, Reinhard E., Kern, Peter M., Stach, Christian, Kalden, Joachim R., Herrmann, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids - pharmacology Annexin A5 - chemistry apoptosis Apoptosis - drug effects Apoptosis - radiation effects Biomarkers - chemistry Cell Line, Tumor flow cytometry Flow Cytometry - methods Fluorescein-5-isothiocyanate - chemistry Humans Jurkat Cells lectin of Narcissus pseudonarcissus Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - radiation effects Narcissus - chemistry Plant Lectins - chemistry short‐term acid treatment Staining and Labeling - methods Time Factors U937 Cells Ultraviolet Rays
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