A depletion-repletion folate bioassay based on growth and tissue folate concentrations of rats
To improve standardization of a folate bioassay, folate-depleted rats were repleted with a folate-free amino acid-based diet supplemented with 29 levels of folic acid. Growth was the main response variable and body tissue folate concentrations were also assessed. Because a positive correlation was o...
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| Veröffentlicht in: | The Journal of nutrition 1993-05, Vol.123 (5), p.926-932 |
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| creator | Clifford, A.J Bills, N.D Peerson, J.M Muller, H.G Burk, G.E Rich, K.D |
| description | To improve standardization of a folate bioassay, folate-depleted rats were repleted with a folate-free amino acid-based diet supplemented with 29 levels of folic acid. Growth was the main response variable and body tissue folate concentrations were also assessed. Because a positive correlation was observed between low levels of dietary folic acid and growth and little or no correlation was observed between high levels and growth, six regression models with a steep slope for low levels and a shallow or zero slope for high levels of dietary folic acid were evaluated. The model referred to as the "two-phase regression" or "change-point" model best described the relationship. Depleted rats needed 674 +/- 71 nmol folic acid/kg diet to reach their full growth potential. This value is biologically sensible, and this regression model is well established in the statistical literature. The change-point model is highly recommended to characterize the growth response, because growth is a functional response and, in the range of 226 to 680 nmol folic acid/kg, this response is linear, which is an additional advantage. Linear responses are easier to interpret because of complicated issues of interpretation and confidence intervals with nonlinearities. Linear regressions described serum and liver folate responses, whereas exponentials described whole blood and carcass folate responses. Depleted rats needed 5920 and 5780 nmol folic acid/kg diet to maximize their whole blood and carcass folates, respectively |
| doi_str_mv | 10.1093/jn/123.5.926 |
| format | Article |
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Growth was the main response variable and body tissue folate concentrations were also assessed. Because a positive correlation was observed between low levels of dietary folic acid and growth and little or no correlation was observed between high levels and growth, six regression models with a steep slope for low levels and a shallow or zero slope for high levels of dietary folic acid were evaluated. The model referred to as the "two-phase regression" or "change-point" model best described the relationship. Depleted rats needed 674 +/- 71 nmol folic acid/kg diet to reach their full growth potential. This value is biologically sensible, and this regression model is well established in the statistical literature. The change-point model is highly recommended to characterize the growth response, because growth is a functional response and, in the range of 226 to 680 nmol folic acid/kg, this response is linear, which is an additional advantage. Linear responses are easier to interpret because of complicated issues of interpretation and confidence intervals with nonlinearities. Linear regressions described serum and liver folate responses, whereas exponentials described whole blood and carcass folate responses. Depleted rats needed 5920 and 5780 nmol folic acid/kg diet to maximize their whole blood and carcass folates, respectively</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/123.5.926</identifier><identifier>PMID: 7683714</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Nutritional Sciences</publisher><subject>ACIDE FOLIQUE ; ACIDO FOLICO ; Amino acids ; ANALISIS ESTADISTICO ; ANALYSE STATISTIQUE ; Animals ; Biological and medical sciences ; Biological Availability ; CARENCE EN VITAMINE ; DEFICIENCIA DE VITAMINAS ; Diet ; DIETA ; DOSAGE BIOLOGIQUE ; ENSAYO BIOLOGICO ; EXPERIMENTACION ; EXPERIMENTATION ; Feeding. Feeding behavior ; Folic Acid - analysis ; Folic Acid - pharmacokinetics ; Folic Acid - pharmacology ; Folic Acid Deficiency - metabolism ; Fundamental and applied biological sciences. Psychology ; Growth - drug effects ; Hydrolysis ; INDICE DE CRECIMIENTO ; Male ; MODELE ; MODELOS ; Models, Theoretical ; Nutritional Requirements ; Polyglutamic Acid - metabolism ; RAT ; RATA ; Rats ; Rats, Sprague-Dawley ; REGIME ALIMENTAIRE ; Regression Analysis ; Rodents ; TAUX DE CROISSANCE ; Tissue Distribution ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vitamin B ; VITAMINAS ; VITAMINE</subject><ispartof>The Journal of nutrition, 1993-05, Vol.123 (5), p.926-932</ispartof><rights>1993 INIST-CNRS</rights><rights>Copyright American Institute of Nutrition May 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-c775f2d0018c68c362210656811779de9a851822c84700f9f3b236430d448ae63</citedby><cites>FETCH-LOGICAL-c465t-c775f2d0018c68c362210656811779de9a851822c84700f9f3b236430d448ae63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4922521$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7683714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clifford, A.J</creatorcontrib><creatorcontrib>Bills, N.D</creatorcontrib><creatorcontrib>Peerson, J.M</creatorcontrib><creatorcontrib>Muller, H.G</creatorcontrib><creatorcontrib>Burk, G.E</creatorcontrib><creatorcontrib>Rich, K.D</creatorcontrib><title>A depletion-repletion folate bioassay based on growth and tissue folate concentrations of rats</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>To improve standardization of a folate bioassay, folate-depleted rats were repleted with a folate-free amino acid-based diet supplemented with 29 levels of folic acid. Growth was the main response variable and body tissue folate concentrations were also assessed. Because a positive correlation was observed between low levels of dietary folic acid and growth and little or no correlation was observed between high levels and growth, six regression models with a steep slope for low levels and a shallow or zero slope for high levels of dietary folic acid were evaluated. The model referred to as the "two-phase regression" or "change-point" model best described the relationship. Depleted rats needed 674 +/- 71 nmol folic acid/kg diet to reach their full growth potential. This value is biologically sensible, and this regression model is well established in the statistical literature. The change-point model is highly recommended to characterize the growth response, because growth is a functional response and, in the range of 226 to 680 nmol folic acid/kg, this response is linear, which is an additional advantage. Linear responses are easier to interpret because of complicated issues of interpretation and confidence intervals with nonlinearities. Linear regressions described serum and liver folate responses, whereas exponentials described whole blood and carcass folate responses. Depleted rats needed 5920 and 5780 nmol folic acid/kg diet to maximize their whole blood and carcass folates, respectively</description><subject>ACIDE FOLIQUE</subject><subject>ACIDO FOLICO</subject><subject>Amino acids</subject><subject>ANALISIS ESTADISTICO</subject><subject>ANALYSE STATISTIQUE</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Availability</subject><subject>CARENCE EN VITAMINE</subject><subject>DEFICIENCIA DE VITAMINAS</subject><subject>Diet</subject><subject>DIETA</subject><subject>DOSAGE BIOLOGIQUE</subject><subject>ENSAYO BIOLOGICO</subject><subject>EXPERIMENTACION</subject><subject>EXPERIMENTATION</subject><subject>Feeding. Feeding behavior</subject><subject>Folic Acid - analysis</subject><subject>Folic Acid - pharmacokinetics</subject><subject>Folic Acid - pharmacology</subject><subject>Folic Acid Deficiency - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth - drug effects</subject><subject>Hydrolysis</subject><subject>INDICE DE CRECIMIENTO</subject><subject>Male</subject><subject>MODELE</subject><subject>MODELOS</subject><subject>Models, Theoretical</subject><subject>Nutritional Requirements</subject><subject>Polyglutamic Acid - metabolism</subject><subject>RAT</subject><subject>RATA</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>REGIME ALIMENTAIRE</subject><subject>Regression Analysis</subject><subject>Rodents</subject><subject>TAUX DE CROISSANCE</subject><subject>Tissue Distribution</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vitamin B</subject><subject>VITAMINAS</subject><subject>VITAMINE</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0EtrGzEUBWARWlLnseuqUBAlZNVxdPXWMoQmKQS6aLONkDVSOmYsudIMJf--Mnay6EoXzqfL5SD0EcgSiGFX63QFlC3F0lB5hBYgOHQSCHmHFoRQ2jGQ8gM6qXVNCAFu9DE6VlIzBXyBnq5xH7ZjmIacuvI64ZhHNwW8GrKr1b3glauhxy14Lvnv9Bu71ONpqHUOr9Tn5EOaitv9rzhH3MZ6ht5HN9ZwfnhP0ePtt183993Dj7vvN9cPnedSTJ1XSkTat_O0l9ozSSkQKaQGUMr0wTgtQFPqNVeERBPZijLJGek51y5Idoou93u3Jf-ZQ53sZqg-jKNLIc_VKqEoV1w1-OU_uM5zSe02C0ZxUELu0Nc98iXXWkK02zJsXHmxQOyuc7tOtnVuhW2dN_75sHNebUL_hg8lt_zikLvq3RiLS36ob4wbSgWFxj7tWXTZuufSyONPw8EQTdg_lY6P4w</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>Clifford, A.J</creator><creator>Bills, N.D</creator><creator>Peerson, J.M</creator><creator>Muller, H.G</creator><creator>Burk, G.E</creator><creator>Rich, K.D</creator><general>American Society for Nutritional Sciences</general><general>American Institute of Nutrition</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>19930501</creationdate><title>A depletion-repletion folate bioassay based on growth and tissue folate concentrations of rats</title><author>Clifford, A.J ; Bills, N.D ; Peerson, J.M ; Muller, H.G ; Burk, G.E ; Rich, K.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-c775f2d0018c68c362210656811779de9a851822c84700f9f3b236430d448ae63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ACIDE FOLIQUE</topic><topic>ACIDO FOLICO</topic><topic>Amino acids</topic><topic>ANALISIS ESTADISTICO</topic><topic>ANALYSE STATISTIQUE</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Availability</topic><topic>CARENCE EN VITAMINE</topic><topic>DEFICIENCIA DE VITAMINAS</topic><topic>Diet</topic><topic>DIETA</topic><topic>DOSAGE BIOLOGIQUE</topic><topic>ENSAYO BIOLOGICO</topic><topic>EXPERIMENTACION</topic><topic>EXPERIMENTATION</topic><topic>Feeding. Feeding behavior</topic><topic>Folic Acid - analysis</topic><topic>Folic Acid - pharmacokinetics</topic><topic>Folic Acid - pharmacology</topic><topic>Folic Acid Deficiency - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth - drug effects</topic><topic>Hydrolysis</topic><topic>INDICE DE CRECIMIENTO</topic><topic>Male</topic><topic>MODELE</topic><topic>MODELOS</topic><topic>Models, Theoretical</topic><topic>Nutritional Requirements</topic><topic>Polyglutamic Acid - metabolism</topic><topic>RAT</topic><topic>RATA</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>REGIME ALIMENTAIRE</topic><topic>Regression Analysis</topic><topic>Rodents</topic><topic>TAUX DE CROISSANCE</topic><topic>Tissue Distribution</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vitamin B</topic><topic>VITAMINAS</topic><topic>VITAMINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clifford, A.J</creatorcontrib><creatorcontrib>Bills, N.D</creatorcontrib><creatorcontrib>Peerson, J.M</creatorcontrib><creatorcontrib>Muller, H.G</creatorcontrib><creatorcontrib>Burk, G.E</creatorcontrib><creatorcontrib>Rich, K.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clifford, A.J</au><au>Bills, N.D</au><au>Peerson, J.M</au><au>Muller, H.G</au><au>Burk, G.E</au><au>Rich, K.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A depletion-repletion folate bioassay based on growth and tissue folate concentrations of rats</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1993-05-01</date><risdate>1993</risdate><volume>123</volume><issue>5</issue><spage>926</spage><epage>932</epage><pages>926-932</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>To improve standardization of a folate bioassay, folate-depleted rats were repleted with a folate-free amino acid-based diet supplemented with 29 levels of folic acid. Growth was the main response variable and body tissue folate concentrations were also assessed. Because a positive correlation was observed between low levels of dietary folic acid and growth and little or no correlation was observed between high levels and growth, six regression models with a steep slope for low levels and a shallow or zero slope for high levels of dietary folic acid were evaluated. The model referred to as the "two-phase regression" or "change-point" model best described the relationship. Depleted rats needed 674 +/- 71 nmol folic acid/kg diet to reach their full growth potential. This value is biologically sensible, and this regression model is well established in the statistical literature. The change-point model is highly recommended to characterize the growth response, because growth is a functional response and, in the range of 226 to 680 nmol folic acid/kg, this response is linear, which is an additional advantage. Linear responses are easier to interpret because of complicated issues of interpretation and confidence intervals with nonlinearities. Linear regressions described serum and liver folate responses, whereas exponentials described whole blood and carcass folate responses. Depleted rats needed 5920 and 5780 nmol folic acid/kg diet to maximize their whole blood and carcass folates, respectively</abstract><cop>Bethesda, MD</cop><pub>American Society for Nutritional Sciences</pub><pmid>7683714</pmid><doi>10.1093/jn/123.5.926</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of nutrition, 1993-05, Vol.123 (5), p.926-932 |
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| subjects | ACIDE FOLIQUE ACIDO FOLICO Amino acids ANALISIS ESTADISTICO ANALYSE STATISTIQUE Animals Biological and medical sciences Biological Availability CARENCE EN VITAMINE DEFICIENCIA DE VITAMINAS Diet DIETA DOSAGE BIOLOGIQUE ENSAYO BIOLOGICO EXPERIMENTACION EXPERIMENTATION Feeding. Feeding behavior Folic Acid - analysis Folic Acid - pharmacokinetics Folic Acid - pharmacology Folic Acid Deficiency - metabolism Fundamental and applied biological sciences. Psychology Growth - drug effects Hydrolysis INDICE DE CRECIMIENTO Male MODELE MODELOS Models, Theoretical Nutritional Requirements Polyglutamic Acid - metabolism RAT RATA Rats Rats, Sprague-Dawley REGIME ALIMENTAIRE Regression Analysis Rodents TAUX DE CROISSANCE Tissue Distribution Vertebrates: anatomy and physiology, studies on body, several organs or systems Vitamin B VITAMINAS VITAMINE |
| title | A depletion-repletion folate bioassay based on growth and tissue folate concentrations of rats |
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