Strong Promoter Activity of Human and Rat Islet Amyloid Polypeptide / Amylin Gene Constructs in Mouse Beta Cells (βTC 3)
Islet amyloid polypeptide (IAPP)(′amylin′) is co-produced with insulin in pancreatic β cells and is the formative polypeptide of pancreatic amyloid in patients with type 2 (non-insulin-dependent) diabetes mellitus. Islet amyloid and type 2 diabetes occur in man, but not in rat. To study transcriptio...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1993-04, Vol.192 (2), p.840-848 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 848 |
|---|---|
| container_issue | 2 |
| container_start_page | 840 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 192 |
| creator | Dewit, L. Vanmansfeld, A.D.M. Vanteeffelen, H.A.A.M. Lips, C.J.M. Hoppener, J.W.M. |
| description | Islet amyloid polypeptide (IAPP)(′amylin′) is co-produced with insulin in pancreatic β cells and is the formative polypeptide of pancreatic amyloid in patients with type 2 (non-insulin-dependent) diabetes mellitus. Islet amyloid and type 2 diabetes occur in man, but not in rat. To study transcription regulation of IAPP gene expression in man and rat, luciferase reporter constructs containing different portions of the upstream region of both IAPP genes were expressed in transfected cells. Both the human and the rat IAPP gene constructs revealed higher promoter activity in β cells (particularly in βTC3 cells) than in non-β cells. In both IAPP genes potential transcription elements, with homology to insulin gene transcription elements, were identified. βTC3 cells provide a good model system in which to study regulation of human and rat IAPP gene expression. |
| doi_str_mv | 10.1006/bbrc.1993.1491 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75721155</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X83714919</els_id><sourcerecordid>75721155</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-1f738e7288f1d6876ea2044bf90399efb7dc71c99ddc367bf1d5ff9191a7e7963</originalsourceid><addsrcrecordid>eNp1kM2KFDEURoMoYzu6dSdkIaKL6knqL8myLXRmYMRBR3AXUsmNRKqSNkkN1Gv5ID6TabuZnasL957v8nEQeknJlhLSX4xj1FsqRLOlraCP0IYSQaqakvYx2pBCVLWg35-iZyn9JITSthdn6Iy3vGWcb9D6Ncfgf-DbGOaQIeKdzu7e5RUHi6-WWXmsvMFfVMbXaYKMd_M6BWfwbZjWPeyzM4Av_m2dx5fgAQ_BpxwXnRMuq09hSYDfQ1Z4gGlK-O2f33cDbt49R0-smhK8OM1z9O3jh7vhqrr5fHk97G4q3fQ8V9SyhgOrObfU9Jz1oGrStqMVpBEC7MiMZlQLYUwJsLFQnbWCCqoYMNE35-jN8e8-hl8LpCxnl3SpojyUbpJ1rKa06wq4PYI6hpQiWLmPblZxlZTIg2t5cC0PruXBdQm8On1exhnMA36SW-6vT3eVtJpsVF679IC1jPVdTQrGjxgUC_cOokzagddgXASdpQnufw3-AtLGmoY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75721155</pqid></control><display><type>article</type><title>Strong Promoter Activity of Human and Rat Islet Amyloid Polypeptide / Amylin Gene Constructs in Mouse Beta Cells (βTC 3)</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Dewit, L. ; Vanmansfeld, A.D.M. ; Vanteeffelen, H.A.A.M. ; Lips, C.J.M. ; Hoppener, J.W.M.</creator><creatorcontrib>Dewit, L. ; Vanmansfeld, A.D.M. ; Vanteeffelen, H.A.A.M. ; Lips, C.J.M. ; Hoppener, J.W.M.</creatorcontrib><description>Islet amyloid polypeptide (IAPP)(′amylin′) is co-produced with insulin in pancreatic β cells and is the formative polypeptide of pancreatic amyloid in patients with type 2 (non-insulin-dependent) diabetes mellitus. Islet amyloid and type 2 diabetes occur in man, but not in rat. To study transcription regulation of IAPP gene expression in man and rat, luciferase reporter constructs containing different portions of the upstream region of both IAPP genes were expressed in transfected cells. Both the human and the rat IAPP gene constructs revealed higher promoter activity in β cells (particularly in βTC3 cells) than in non-β cells. In both IAPP genes potential transcription elements, with homology to insulin gene transcription elements, were identified. βTC3 cells provide a good model system in which to study regulation of human and rat IAPP gene expression.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1491</identifier><identifier>PMID: 8484788</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amyloid - genetics ; Animals ; Base Sequence ; Biological and medical sciences ; DNA ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Humans ; Islet Amyloid Polypeptide ; Islets of Langerhans - metabolism ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Promoter Regions, Genetic ; Rats ; Tumor Cells, Cultured</subject><ispartof>Biochemical and biophysical research communications, 1993-04, Vol.192 (2), p.840-848</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-1f738e7288f1d6876ea2044bf90399efb7dc71c99ddc367bf1d5ff9191a7e7963</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X83714919$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4776520$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8484788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dewit, L.</creatorcontrib><creatorcontrib>Vanmansfeld, A.D.M.</creatorcontrib><creatorcontrib>Vanteeffelen, H.A.A.M.</creatorcontrib><creatorcontrib>Lips, C.J.M.</creatorcontrib><creatorcontrib>Hoppener, J.W.M.</creatorcontrib><title>Strong Promoter Activity of Human and Rat Islet Amyloid Polypeptide / Amylin Gene Constructs in Mouse Beta Cells (βTC 3)</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Islet amyloid polypeptide (IAPP)(′amylin′) is co-produced with insulin in pancreatic β cells and is the formative polypeptide of pancreatic amyloid in patients with type 2 (non-insulin-dependent) diabetes mellitus. Islet amyloid and type 2 diabetes occur in man, but not in rat. To study transcription regulation of IAPP gene expression in man and rat, luciferase reporter constructs containing different portions of the upstream region of both IAPP genes were expressed in transfected cells. Both the human and the rat IAPP gene constructs revealed higher promoter activity in β cells (particularly in βTC3 cells) than in non-β cells. In both IAPP genes potential transcription elements, with homology to insulin gene transcription elements, were identified. βTC3 cells provide a good model system in which to study regulation of human and rat IAPP gene expression.</description><subject>Amyloid - genetics</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Islet Amyloid Polypeptide</subject><subject>Islets of Langerhans - metabolism</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions, Genetic</subject><subject>Rats</subject><subject>Tumor Cells, Cultured</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM2KFDEURoMoYzu6dSdkIaKL6knqL8myLXRmYMRBR3AXUsmNRKqSNkkN1Gv5ID6TabuZnasL957v8nEQeknJlhLSX4xj1FsqRLOlraCP0IYSQaqakvYx2pBCVLWg35-iZyn9JITSthdn6Iy3vGWcb9D6Ncfgf-DbGOaQIeKdzu7e5RUHi6-WWXmsvMFfVMbXaYKMd_M6BWfwbZjWPeyzM4Av_m2dx5fgAQ_BpxwXnRMuq09hSYDfQ1Z4gGlK-O2f33cDbt49R0-smhK8OM1z9O3jh7vhqrr5fHk97G4q3fQ8V9SyhgOrObfU9Jz1oGrStqMVpBEC7MiMZlQLYUwJsLFQnbWCCqoYMNE35-jN8e8-hl8LpCxnl3SpojyUbpJ1rKa06wq4PYI6hpQiWLmPblZxlZTIg2t5cC0PruXBdQm8On1exhnMA36SW-6vT3eVtJpsVF679IC1jPVdTQrGjxgUC_cOokzagddgXASdpQnufw3-AtLGmoY</recordid><startdate>19930430</startdate><enddate>19930430</enddate><creator>Dewit, L.</creator><creator>Vanmansfeld, A.D.M.</creator><creator>Vanteeffelen, H.A.A.M.</creator><creator>Lips, C.J.M.</creator><creator>Hoppener, J.W.M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930430</creationdate><title>Strong Promoter Activity of Human and Rat Islet Amyloid Polypeptide / Amylin Gene Constructs in Mouse Beta Cells (βTC 3)</title><author>Dewit, L. ; Vanmansfeld, A.D.M. ; Vanteeffelen, H.A.A.M. ; Lips, C.J.M. ; Hoppener, J.W.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-1f738e7288f1d6876ea2044bf90399efb7dc71c99ddc367bf1d5ff9191a7e7963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amyloid - genetics</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Islet Amyloid Polypeptide</topic><topic>Islets of Langerhans - metabolism</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions, Genetic</topic><topic>Rats</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dewit, L.</creatorcontrib><creatorcontrib>Vanmansfeld, A.D.M.</creatorcontrib><creatorcontrib>Vanteeffelen, H.A.A.M.</creatorcontrib><creatorcontrib>Lips, C.J.M.</creatorcontrib><creatorcontrib>Hoppener, J.W.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dewit, L.</au><au>Vanmansfeld, A.D.M.</au><au>Vanteeffelen, H.A.A.M.</au><au>Lips, C.J.M.</au><au>Hoppener, J.W.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Strong Promoter Activity of Human and Rat Islet Amyloid Polypeptide / Amylin Gene Constructs in Mouse Beta Cells (βTC 3)</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-04-30</date><risdate>1993</risdate><volume>192</volume><issue>2</issue><spage>840</spage><epage>848</epage><pages>840-848</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Islet amyloid polypeptide (IAPP)(′amylin′) is co-produced with insulin in pancreatic β cells and is the formative polypeptide of pancreatic amyloid in patients with type 2 (non-insulin-dependent) diabetes mellitus. Islet amyloid and type 2 diabetes occur in man, but not in rat. To study transcription regulation of IAPP gene expression in man and rat, luciferase reporter constructs containing different portions of the upstream region of both IAPP genes were expressed in transfected cells. Both the human and the rat IAPP gene constructs revealed higher promoter activity in β cells (particularly in βTC3 cells) than in non-β cells. In both IAPP genes potential transcription elements, with homology to insulin gene transcription elements, were identified. βTC3 cells provide a good model system in which to study regulation of human and rat IAPP gene expression.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8484788</pmid><doi>10.1006/bbrc.1993.1491</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1993-04, Vol.192 (2), p.840-848 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75721155 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amyloid - genetics Animals Base Sequence Biological and medical sciences DNA Fundamental and applied biological sciences. Psychology Gene Expression Humans Islet Amyloid Polypeptide Islets of Langerhans - metabolism Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Promoter Regions, Genetic Rats Tumor Cells, Cultured |
| title | Strong Promoter Activity of Human and Rat Islet Amyloid Polypeptide / Amylin Gene Constructs in Mouse Beta Cells (βTC 3) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T17%3A03%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Strong%20Promoter%20Activity%20of%20Human%20and%20Rat%20Islet%20Amyloid%20Polypeptide%20/%20Amylin%20Gene%20Constructs%20in%20Mouse%20Beta%20Cells%20(%CE%B2TC%203)&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Dewit,%20L.&rft.date=1993-04-30&rft.volume=192&rft.issue=2&rft.spage=840&rft.epage=848&rft.pages=840-848&rft.issn=0006-291X&rft.eissn=1090-2104&rft.coden=BBRCA9&rft_id=info:doi/10.1006/bbrc.1993.1491&rft_dat=%3Cproquest_cross%3E75721155%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75721155&rft_id=info:pmid/8484788&rft_els_id=S0006291X83714919&rfr_iscdi=true |