Monoclonal antibody-based competitive ELISA for simultaneous detection of rinderpest virus and peste des petits ruminants virus antibodies

An experimental competitive enzyme-linked immunosorbent assay (morbillivirus cELISA) using a recombinant N antigen (rRPV N) expressed in a baculovirus and a ruminant morbillivirus (RPV and PPRV)-specific monoclonal antibody (P-13A9) was developed for simultaneous detection of rinderpest virus (RPV)...

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Veröffentlicht in:	Veterinary microbiology 2003-10, Vol.96 (1), p.1-16
Hauptverfasser:	Choi, Kang-Seuk, Nah, Jin-Ju, Choi, Cheong-Up, Ko, Young-Joon, Sohn, Hyun-Joo, Libeau, Genevieve, Kang, Shien-Young, Joo, Yi-Seok
Format:	Artikel
Sprache:	eng
Schlagworte:	AN protein Animals antibodies Antibodies, Monoclonal Antibodies, Viral - blood antiserum Applied microbiology Biological and medical sciences Blotting, Western - veterinary Cattle Cattle Diseases - diagnosis Competitive ELISA Electrophoresis, Polyacrylamide Gel - veterinary enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary epitopes Fundamental and applied biological sciences. Psychology Goat Diseases - diagnosis Goats Microbiology Miscellaneous N protein Peste des petits ruminants Peste-des-Petits-Ruminants - diagnosis Peste-des-petits-ruminants virus Peste-des-petits-ruminants virus - immunology Recombinant N protein Rinderpest Rinderpest - diagnosis Rinderpest virus Rinderpest virus - immunology Ruminant morbillivirus Sensitivity and Specificity Sheep Sheep Diseases - diagnosis Simultaneous detection Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology viruses
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