Inhibition of the SAPK/JNK pathway blocks the stimulatory effects of glutamine on fluid secretion by the Malpighian tubules of Rhodnius prolixus
Physiological levels of amino acids such as glutamine, glutamate, aspartate and proline increase the rates of fluid secretion and ion transport by serotonin-stimulated Malpighian tubules (MTs) of Rhodnius prolixus. Here, we examine the proposal that the effects of glutamine are mediated through acti...
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| Veröffentlicht in: | Journal of insect physiology 2003-10, Vol.49 (10), p.897-906 |
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| container_title | Journal of insect physiology |
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| creator | Hazel, Matthew H Christensen, Robert J O’Donnell, Michael J |
| description | Physiological levels of amino acids such as glutamine, glutamate, aspartate and proline increase the rates of fluid secretion and ion transport by serotonin-stimulated Malpighian tubules (MTs) of
Rhodnius
prolixus. Here, we examine the proposal that the effects of glutamine are mediated through activation of specific kinases to produce the observed increases in fluid secretion. The glutamine-dependent increase in MT fluid secretion rate was blocked by two chemically unrelated inhibitors of the stress activated protein kinase (SAPK) pathway, SP600125 and dicumarol. Inhibitors of phosphatidyl inositol-3 kinase, p38 mitogen activated protein kinase (MAPK), extracellular-signal regulated kinases and MAPK kinase did not block glutamine’s effects on fluid secretion rate when applied at commonly used concentrations. Inhibitors of protein kinase A or C reduced fluid secretion rates of serotonin-stimulated MTs, but did not block the response to glutamine. The glutamine-dependent increase in fluid secretion was also insensitive to cytoskeletal disrupting agents and protein synthesis inhibitors. Results of this study are the first to suggest a role for the SAPK pathway in the control of fluid secretion rates by insect MTs. |
| doi_str_mv | 10.1016/S0022-1910(03)00138-0 |
| format | Article |
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Rhodnius
prolixus. Here, we examine the proposal that the effects of glutamine are mediated through activation of specific kinases to produce the observed increases in fluid secretion. The glutamine-dependent increase in MT fluid secretion rate was blocked by two chemically unrelated inhibitors of the stress activated protein kinase (SAPK) pathway, SP600125 and dicumarol. Inhibitors of phosphatidyl inositol-3 kinase, p38 mitogen activated protein kinase (MAPK), extracellular-signal regulated kinases and MAPK kinase did not block glutamine’s effects on fluid secretion rate when applied at commonly used concentrations. Inhibitors of protein kinase A or C reduced fluid secretion rates of serotonin-stimulated MTs, but did not block the response to glutamine. The glutamine-dependent increase in fluid secretion was also insensitive to cytoskeletal disrupting agents and protein synthesis inhibitors. Results of this study are the first to suggest a role for the SAPK pathway in the control of fluid secretion rates by insect MTs.</description><identifier>ISSN: 0022-1910</identifier><identifier>EISSN: 1879-1611</identifier><identifier>DOI: 10.1016/S0022-1910(03)00138-0</identifier><identifier>PMID: 14511822</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Anthracenes - pharmacology ; biochemical pathways ; c-jun terminal kinase ; dicoumarol ; Dicumarol ; Dicumarol - pharmacology ; enzyme inhibitors ; Enzyme Inhibitors - pharmacology ; Glutamine ; Glutamine - antagonists & inhibitors ; Glutamine - metabolism ; inhibitors ; insect biochemistry ; kinase inhibitors ; kinases ; Malpighian tubule ; Malpighian tubules ; Malpighian Tubules - metabolism ; Mitogen-Activated Protein Kinase 8 ; Mitogen-Activated Protein Kinases - antagonists & inhibitors ; Mitogen-Activated Protein Kinases - metabolism ; physiological regulation ; Protein kinase A ; Protein kinase C ; protein kinases ; protein synthesis inhibitors ; Protein Synthesis Inhibitors - pharmacology ; Reduviidae ; Rhodnius - physiology ; Rhodnius prolixus ; secretion ; Secretory Rate - drug effects ; Secretory Rate - physiology ; serotonin ; Serotonin - metabolism ; SP600125 ; Stress activated protein kinase</subject><ispartof>Journal of insect physiology, 2003-10, Vol.49 (10), p.897-906</ispartof><rights>2003 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-df52a996ef2ca528bcfe1d339772f6debb6cb875c85edfb75179d712d26df6b03</citedby><cites>FETCH-LOGICAL-c468t-df52a996ef2ca528bcfe1d339772f6debb6cb875c85edfb75179d712d26df6b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022191003001380$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14511822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hazel, Matthew H</creatorcontrib><creatorcontrib>Christensen, Robert J</creatorcontrib><creatorcontrib>O’Donnell, Michael J</creatorcontrib><title>Inhibition of the SAPK/JNK pathway blocks the stimulatory effects of glutamine on fluid secretion by the Malpighian tubules of Rhodnius prolixus</title><title>Journal of insect physiology</title><addtitle>J Insect Physiol</addtitle><description>Physiological levels of amino acids such as glutamine, glutamate, aspartate and proline increase the rates of fluid secretion and ion transport by serotonin-stimulated Malpighian tubules (MTs) of
Rhodnius
prolixus. Here, we examine the proposal that the effects of glutamine are mediated through activation of specific kinases to produce the observed increases in fluid secretion. The glutamine-dependent increase in MT fluid secretion rate was blocked by two chemically unrelated inhibitors of the stress activated protein kinase (SAPK) pathway, SP600125 and dicumarol. Inhibitors of phosphatidyl inositol-3 kinase, p38 mitogen activated protein kinase (MAPK), extracellular-signal regulated kinases and MAPK kinase did not block glutamine’s effects on fluid secretion rate when applied at commonly used concentrations. Inhibitors of protein kinase A or C reduced fluid secretion rates of serotonin-stimulated MTs, but did not block the response to glutamine. The glutamine-dependent increase in fluid secretion was also insensitive to cytoskeletal disrupting agents and protein synthesis inhibitors. Results of this study are the first to suggest a role for the SAPK pathway in the control of fluid secretion rates by insect MTs.</description><subject>Animals</subject><subject>Anthracenes - pharmacology</subject><subject>biochemical pathways</subject><subject>c-jun terminal kinase</subject><subject>dicoumarol</subject><subject>Dicumarol</subject><subject>Dicumarol - pharmacology</subject><subject>enzyme inhibitors</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Glutamine</subject><subject>Glutamine - antagonists & inhibitors</subject><subject>Glutamine - metabolism</subject><subject>inhibitors</subject><subject>insect biochemistry</subject><subject>kinase inhibitors</subject><subject>kinases</subject><subject>Malpighian tubule</subject><subject>Malpighian tubules</subject><subject>Malpighian Tubules - metabolism</subject><subject>Mitogen-Activated Protein Kinase 8</subject><subject>Mitogen-Activated Protein Kinases - antagonists & inhibitors</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>physiological regulation</subject><subject>Protein kinase A</subject><subject>Protein kinase C</subject><subject>protein kinases</subject><subject>protein synthesis inhibitors</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>Reduviidae</subject><subject>Rhodnius - physiology</subject><subject>Rhodnius prolixus</subject><subject>secretion</subject><subject>Secretory Rate - drug effects</subject><subject>Secretory Rate - physiology</subject><subject>serotonin</subject><subject>Serotonin - metabolism</subject><subject>SP600125</subject><subject>Stress activated protein kinase</subject><issn>0022-1910</issn><issn>1879-1611</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EokPhEYCsECxCbSd2khWqKi6l5SKGri1fjicGJx7sGJi34JHJZEaw7MoLf99_js6P0GOCXxJM-NkaY0pL0hH8HFcvMCZVW-I7aEXapisJJ-QuWv1DTtCDlL5hjBlv2X10QmpGSEvpCv25HHun3OTCWARbTD0U6_PPV2fvP14VWzn1v-SuUD7o72n5S5MbspdTiLsCrAU9pb228XmSgxuhmGOsz84UCXSEJVbtFvWD9Fu36Z0ciymr7GExv_TBjC6nYhuDd79zeojuWekTPDq-p-jmzeuvF-_K609vLy_Or0td83YqjWVUdh0HS7VktFXaAjFV1TUNtdyAUlyrtmG6ZWCsahhpOtMQaig3litcnaJnh9x58I8MaRKDSxq8lyOEnETDGorrproVnO_LWY3ZDLIDqGNIKYIV2-gGGXeCYLHvTCyd7QUscCWWzsR-kyfHAVkNYP5bx5Jm4OkBsDIIuYkuiZs1nW1MMG1JV8_EqwMB88V-OogiaQejBuPi3JEwwd2yxF9CwbIC</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Hazel, Matthew H</creator><creator>Christensen, Robert J</creator><creator>O’Donnell, Michael J</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Inhibition of the SAPK/JNK pathway blocks the stimulatory effects of glutamine on fluid secretion by the Malpighian tubules of Rhodnius prolixus</title><author>Hazel, Matthew H ; Christensen, Robert J ; O’Donnell, Michael J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-df52a996ef2ca528bcfe1d339772f6debb6cb875c85edfb75179d712d26df6b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Anthracenes - pharmacology</topic><topic>biochemical pathways</topic><topic>c-jun terminal kinase</topic><topic>dicoumarol</topic><topic>Dicumarol</topic><topic>Dicumarol - pharmacology</topic><topic>enzyme inhibitors</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Glutamine</topic><topic>Glutamine - antagonists & inhibitors</topic><topic>Glutamine - metabolism</topic><topic>inhibitors</topic><topic>insect biochemistry</topic><topic>kinase inhibitors</topic><topic>kinases</topic><topic>Malpighian tubule</topic><topic>Malpighian tubules</topic><topic>Malpighian Tubules - metabolism</topic><topic>Mitogen-Activated Protein Kinase 8</topic><topic>Mitogen-Activated Protein Kinases - antagonists & inhibitors</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>physiological regulation</topic><topic>Protein kinase A</topic><topic>Protein kinase C</topic><topic>protein kinases</topic><topic>protein synthesis inhibitors</topic><topic>Protein Synthesis Inhibitors - pharmacology</topic><topic>Reduviidae</topic><topic>Rhodnius - physiology</topic><topic>Rhodnius prolixus</topic><topic>secretion</topic><topic>Secretory Rate - drug effects</topic><topic>Secretory Rate - physiology</topic><topic>serotonin</topic><topic>Serotonin - metabolism</topic><topic>SP600125</topic><topic>Stress activated protein kinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hazel, Matthew H</creatorcontrib><creatorcontrib>Christensen, Robert J</creatorcontrib><creatorcontrib>O’Donnell, Michael J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of insect physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hazel, Matthew H</au><au>Christensen, Robert J</au><au>O’Donnell, Michael J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of the SAPK/JNK pathway blocks the stimulatory effects of glutamine on fluid secretion by the Malpighian tubules of Rhodnius prolixus</atitle><jtitle>Journal of insect physiology</jtitle><addtitle>J Insect Physiol</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>49</volume><issue>10</issue><spage>897</spage><epage>906</epage><pages>897-906</pages><issn>0022-1910</issn><eissn>1879-1611</eissn><abstract>Physiological levels of amino acids such as glutamine, glutamate, aspartate and proline increase the rates of fluid secretion and ion transport by serotonin-stimulated Malpighian tubules (MTs) of
Rhodnius
prolixus. Here, we examine the proposal that the effects of glutamine are mediated through activation of specific kinases to produce the observed increases in fluid secretion. The glutamine-dependent increase in MT fluid secretion rate was blocked by two chemically unrelated inhibitors of the stress activated protein kinase (SAPK) pathway, SP600125 and dicumarol. Inhibitors of phosphatidyl inositol-3 kinase, p38 mitogen activated protein kinase (MAPK), extracellular-signal regulated kinases and MAPK kinase did not block glutamine’s effects on fluid secretion rate when applied at commonly used concentrations. Inhibitors of protein kinase A or C reduced fluid secretion rates of serotonin-stimulated MTs, but did not block the response to glutamine. The glutamine-dependent increase in fluid secretion was also insensitive to cytoskeletal disrupting agents and protein synthesis inhibitors. Results of this study are the first to suggest a role for the SAPK pathway in the control of fluid secretion rates by insect MTs.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>14511822</pmid><doi>10.1016/S0022-1910(03)00138-0</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Anthracenes - pharmacology biochemical pathways c-jun terminal kinase dicoumarol Dicumarol Dicumarol - pharmacology enzyme inhibitors Enzyme Inhibitors - pharmacology Glutamine Glutamine - antagonists & inhibitors Glutamine - metabolism inhibitors insect biochemistry kinase inhibitors kinases Malpighian tubule Malpighian tubules Malpighian Tubules - metabolism Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases - antagonists & inhibitors Mitogen-Activated Protein Kinases - metabolism physiological regulation Protein kinase A Protein kinase C protein kinases protein synthesis inhibitors Protein Synthesis Inhibitors - pharmacology Reduviidae Rhodnius - physiology Rhodnius prolixus secretion Secretory Rate - drug effects Secretory Rate - physiology serotonin Serotonin - metabolism SP600125 Stress activated protein kinase |
| title | Inhibition of the SAPK/JNK pathway blocks the stimulatory effects of glutamine on fluid secretion by the Malpighian tubules of Rhodnius prolixus |
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