Pattern of lactate production and steroidogenesis during growth and maturation of mouse ovarian follicles in vitro
A model culture system for the study of follicular metabolism has been developed from existing methods of whole-follicle culture. The modified system mimics the in vivo growth and maturation of mouse ovarian follicles from primary to preovulatory stages and the modulating influences of the LH and FS...
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| Veröffentlicht in: | Biology of reproduction 1993-04, Vol.48 (4), p.798-806 |
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| container_title | Biology of reproduction |
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| creator | BOLAND, N. I HUMPHERSON, P. G LEESE, H. J GOSDEN, R. G |
| description | A model culture system for the study of follicular metabolism has been developed from existing methods of whole-follicle culture.
The modified system mimics the in vivo growth and maturation of mouse ovarian follicles from primary to preovulatory stages
and the modulating influences of the LH and FSH. This is the first study to demonstrate ovulation in vitro from individual
ovarian follicles. The pattern of follicular lactate production relative to steroidogenesis was studied throughout in vitro
follicular development over a period of 6 days. Twenty-four-hour samples of medium from individual follicles were analyzed
for lactate and pyruvate by an automated analytical technique, and for estradiol and progesterone by an immunoenzymatic method.
Follicles produced remarkably large quantities of lactate and estradiol during FSH-stimulated development in vitro. LH further
stimulated lactate production but resulted in a significant decrease in estradiol secretion and an ovulation rate of 30%.
Progesterone production was not detectable throughout the culture period, and follicles showed no evidence of pyruvate uptake.
These findings demonstrate the validity of using this model culture system for the study of follicular metabolism and provide
new information on the pattern of carbohydrate metabolism relative to steroidogenesis during follicular growth and maturation. |
| doi_str_mv | 10.1095/biolreprod48.4.798 |
| format | Article |
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The modified system mimics the in vivo growth and maturation of mouse ovarian follicles from primary to preovulatory stages
and the modulating influences of the LH and FSH. This is the first study to demonstrate ovulation in vitro from individual
ovarian follicles. The pattern of follicular lactate production relative to steroidogenesis was studied throughout in vitro
follicular development over a period of 6 days. Twenty-four-hour samples of medium from individual follicles were analyzed
for lactate and pyruvate by an automated analytical technique, and for estradiol and progesterone by an immunoenzymatic method.
Follicles produced remarkably large quantities of lactate and estradiol during FSH-stimulated development in vitro. LH further
stimulated lactate production but resulted in a significant decrease in estradiol secretion and an ovulation rate of 30%.
Progesterone production was not detectable throughout the culture period, and follicles showed no evidence of pyruvate uptake.
These findings demonstrate the validity of using this model culture system for the study of follicular metabolism and provide
new information on the pattern of carbohydrate metabolism relative to steroidogenesis during follicular growth and maturation.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod48.4.798</identifier><identifier>PMID: 8485244</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Energy Metabolism ; Estradiol - biosynthesis ; Female ; Follicle Stimulating Hormone - pharmacology ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Lactates - biosynthesis ; Lactic Acid ; Luteinizing Hormone - pharmacology ; Mammalian female genital system ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Morphology. Physiology ; Ovarian Follicle - drug effects ; Ovarian Follicle - metabolism ; Ovarian Follicle - physiology ; Progesterone - biosynthesis ; Pyruvates - metabolism ; Pyruvic Acid ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1993-04, Vol.48 (4), p.798-806</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4710768$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8485244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BOLAND, N. I</creatorcontrib><creatorcontrib>HUMPHERSON, P. G</creatorcontrib><creatorcontrib>LEESE, H. J</creatorcontrib><creatorcontrib>GOSDEN, R. G</creatorcontrib><title>Pattern of lactate production and steroidogenesis during growth and maturation of mouse ovarian follicles in vitro</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A model culture system for the study of follicular metabolism has been developed from existing methods of whole-follicle culture.
The modified system mimics the in vivo growth and maturation of mouse ovarian follicles from primary to preovulatory stages
and the modulating influences of the LH and FSH. This is the first study to demonstrate ovulation in vitro from individual
ovarian follicles. The pattern of follicular lactate production relative to steroidogenesis was studied throughout in vitro
follicular development over a period of 6 days. Twenty-four-hour samples of medium from individual follicles were analyzed
for lactate and pyruvate by an automated analytical technique, and for estradiol and progesterone by an immunoenzymatic method.
Follicles produced remarkably large quantities of lactate and estradiol during FSH-stimulated development in vitro. LH further
stimulated lactate production but resulted in a significant decrease in estradiol secretion and an ovulation rate of 30%.
Progesterone production was not detectable throughout the culture period, and follicles showed no evidence of pyruvate uptake.
These findings demonstrate the validity of using this model culture system for the study of follicular metabolism and provide
new information on the pattern of carbohydrate metabolism relative to steroidogenesis during follicular growth and maturation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Energy Metabolism</subject><subject>Estradiol - biosynthesis</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Lactates - biosynthesis</subject><subject>Lactic Acid</subject><subject>Luteinizing Hormone - pharmacology</subject><subject>Mammalian female genital system</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Morphology. Physiology</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - metabolism</subject><subject>Ovarian Follicle - physiology</subject><subject>Progesterone - biosynthesis</subject><subject>Pyruvates - metabolism</subject><subject>Pyruvic Acid</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU1r3DAQhkVISTeb_IFAQIcmN2_1acnHsqQfsNAe2rMZ2_KugixtJTmm_77KZslBzOF99MxohNAdJRtKGvm5s8FFc4xhEHojNqrRF2hFJWsqxWp9iVaEkLrivOYf0XVKz4RQwRm_QldaaMmEWKH4C3I20eMwYgd9hmzwq3Dusw0egx9wKnmwQ9gbb5JNeJij9Xu8j2HJhxMxQZ4jnC4UzRTmZHB4gWjB4zE4Z3tnErYev9gcww36MIJL5vZc1-jP16ff2-_V7ue3H9svu-pANcmVUlwCN1APfGxozUXDSNcxBbIXklGqO0VZB2M5XYlq1lPCOVMNSFmXhfA1enzzlvf8nU3K7WRTb5wDb8qIrZKK6uIo4P0ZnLvJDO0x2gniv_a8pJJ_OueQenBjBN_b9I4JRYmqX_s9vGEHuz8sNpo2TeBckfJ2WRahW9GWL-L_AbJmhrE</recordid><startdate>199304</startdate><enddate>199304</enddate><creator>BOLAND, N. I</creator><creator>HUMPHERSON, P. G</creator><creator>LEESE, H. J</creator><creator>GOSDEN, R. G</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199304</creationdate><title>Pattern of lactate production and steroidogenesis during growth and maturation of mouse ovarian follicles in vitro</title><author>BOLAND, N. I ; HUMPHERSON, P. G ; LEESE, H. J ; GOSDEN, R. G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h180t-7735a3ea6d3f91634920bb27a5c452118b712baf2bab92062c1033279a556d483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Energy Metabolism</topic><topic>Estradiol - biosynthesis</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Lactates - biosynthesis</topic><topic>Lactic Acid</topic><topic>Luteinizing Hormone - pharmacology</topic><topic>Mammalian female genital system</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Morphology. Physiology</topic><topic>Ovarian Follicle - drug effects</topic><topic>Ovarian Follicle - metabolism</topic><topic>Ovarian Follicle - physiology</topic><topic>Progesterone - biosynthesis</topic><topic>Pyruvates - metabolism</topic><topic>Pyruvic Acid</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BOLAND, N. I</creatorcontrib><creatorcontrib>HUMPHERSON, P. G</creatorcontrib><creatorcontrib>LEESE, H. J</creatorcontrib><creatorcontrib>GOSDEN, R. G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BOLAND, N. I</au><au>HUMPHERSON, P. G</au><au>LEESE, H. J</au><au>GOSDEN, R. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pattern of lactate production and steroidogenesis during growth and maturation of mouse ovarian follicles in vitro</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1993-04</date><risdate>1993</risdate><volume>48</volume><issue>4</issue><spage>798</spage><epage>806</epage><pages>798-806</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>A model culture system for the study of follicular metabolism has been developed from existing methods of whole-follicle culture.
The modified system mimics the in vivo growth and maturation of mouse ovarian follicles from primary to preovulatory stages
and the modulating influences of the LH and FSH. This is the first study to demonstrate ovulation in vitro from individual
ovarian follicles. The pattern of follicular lactate production relative to steroidogenesis was studied throughout in vitro
follicular development over a period of 6 days. Twenty-four-hour samples of medium from individual follicles were analyzed
for lactate and pyruvate by an automated analytical technique, and for estradiol and progesterone by an immunoenzymatic method.
Follicles produced remarkably large quantities of lactate and estradiol during FSH-stimulated development in vitro. LH further
stimulated lactate production but resulted in a significant decrease in estradiol secretion and an ovulation rate of 30%.
Progesterone production was not detectable throughout the culture period, and follicles showed no evidence of pyruvate uptake.
These findings demonstrate the validity of using this model culture system for the study of follicular metabolism and provide
new information on the pattern of carbohydrate metabolism relative to steroidogenesis during follicular growth and maturation.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>8485244</pmid><doi>10.1095/biolreprod48.4.798</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1993-04, Vol.48 (4), p.798-806 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Energy Metabolism Estradiol - biosynthesis Female Follicle Stimulating Hormone - pharmacology Fundamental and applied biological sciences. Psychology In Vitro Techniques Lactates - biosynthesis Lactic Acid Luteinizing Hormone - pharmacology Mammalian female genital system Mice Mice, Inbred C57BL Mice, Inbred CBA Morphology. Physiology Ovarian Follicle - drug effects Ovarian Follicle - metabolism Ovarian Follicle - physiology Progesterone - biosynthesis Pyruvates - metabolism Pyruvic Acid Vertebrates: reproduction |
| title | Pattern of lactate production and steroidogenesis during growth and maturation of mouse ovarian follicles in vitro |
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