A Nucleosome-Free dG-dC-Rich Sequence Element Promotes Constitutive Transcription of the Essential Yeast RIO1 Gene
RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence eleme...
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| Veröffentlicht in: | Biological chemistry 2003-09, Vol.384 (9), p.1287-1292 |
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| creator | Angermayr, M. Schwerdtfeger, K. Bandlow, W. |
| description | RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence element, that is located 40 to 48 bp upstream the single site of mRNA initiation, is essential and presumably constitutes the basal promoter. In addition, we demonstrate here that this promoter element comprises a nucleosomefree gap which is centered at the dG-dC tract and flanked by two positioned nucleosomes. This element is both, necessary and sufficient, for basal transcription initiation at the RIO1 promoter and, thus, constitutes a novel type of core promoter element. |
| doi_str_mv | 10.1515/BC.2003.143 |
| format | Article |
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Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence element, that is located 40 to 48 bp upstream the single site of mRNA initiation, is essential and presumably constitutes the basal promoter. In addition, we demonstrate here that this promoter element comprises a nucleosomefree gap which is centered at the dG-dC tract and flanked by two positioned nucleosomes. 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Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence element, that is located 40 to 48 bp upstream the single site of mRNA initiation, is essential and presumably constitutes the basal promoter. In addition, we demonstrate here that this promoter element comprises a nucleosomefree gap which is centered at the dG-dC tract and flanked by two positioned nucleosomes. This element is both, necessary and sufficient, for basal transcription initiation at the RIO1 promoter and, thus, constitutes a novel type of core promoter element.</description><subject>Chromatin</subject><subject>GC Rich Sequence</subject><subject>Mutation</subject><subject>Nucleosomes</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Transcription, Genetic</subject><issn>1431-6730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1P5DAQQF2ADlio6E-uaFAWfyRxXEIEe0gIFliku2ssrzMRhiRebAfd_Xu82hWUNDPFvBnNvEHomJIpLWhxdlFPGSF8SnO-g_ZTpFkpONlDByG8EEIqkvMfaI_mCZaS7CN_jm9H04ELrofsygPgZpY1dfZgzTN-hLcRBgP4soMehojn3vUuQsC1G0K0cYz2HfDC6yEYb1fRugG7Fsfn1BJC6rC6w39Ah4gfru8onsEAh2i31V2Ao22eoKery0X9K7u5m13X5zeZ4ZWMmSyhKnXR0tLwRjaEglyvzRmUcknAlFQasVyWJQFW5ZqxJq8ka0nDOGdcED5BJ5u5K-_SGSGq3gYDXacHcGNQohA0-RDfgoyyguaiSODpBjTeheChVStve-3_K0rU2r-6qNXav0rmE_1zO3Zc9tB8sVv5Ccg2gA0R_n3WtX9V6WmiUPeLXN3Xv-u_ck6U4B_gyo-t</recordid><startdate>20030928</startdate><enddate>20030928</enddate><creator>Angermayr, M.</creator><creator>Schwerdtfeger, K.</creator><creator>Bandlow, W.</creator><general>Walter de Gruyter</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030928</creationdate><title>A Nucleosome-Free dG-dC-Rich Sequence Element Promotes Constitutive Transcription of the Essential Yeast RIO1 Gene</title><author>Angermayr, M. ; Schwerdtfeger, K. ; Bandlow, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-96e86a5f16c3d9d01e9145132e69b0ec619c7bb660e284a22d4892f0d23323703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Chromatin</topic><topic>GC Rich Sequence</topic><topic>Mutation</topic><topic>Nucleosomes</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angermayr, M.</creatorcontrib><creatorcontrib>Schwerdtfeger, K.</creatorcontrib><creatorcontrib>Bandlow, W.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angermayr, M.</au><au>Schwerdtfeger, K.</au><au>Bandlow, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Nucleosome-Free dG-dC-Rich Sequence Element Promotes Constitutive Transcription of the Essential Yeast RIO1 Gene</atitle><jtitle>Biological chemistry</jtitle><addtitle>Biological Chemistry</addtitle><date>2003-09-28</date><risdate>2003</risdate><volume>384</volume><issue>9</issue><spage>1287</spage><epage>1292</epage><pages>1287-1292</pages><issn>1431-6730</issn><abstract>RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. 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| ispartof | Biological chemistry, 2003-09, Vol.384 (9), p.1287-1292 |
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| language | eng |
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| source | MEDLINE; De Gruyter journals |
| subjects | Chromatin GC Rich Sequence Mutation Nucleosomes Promoter Regions, Genetic - genetics Protein-Serine-Threonine Kinases - genetics Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics Transcription, Genetic |
| title | A Nucleosome-Free dG-dC-Rich Sequence Element Promotes Constitutive Transcription of the Essential Yeast RIO1 Gene |
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