Manganese exposure to PC-12 cells alters triacylglycerol metabolism and promotes neurite outgrowth
Exposure of PC-12 cells to MnCl 2 (10–100 μM for 1 to 3 days) resulted in both morphological and metabolic changes as indicated by promotion of neurite outgrowth and an increased incorporation of labeled fatty acids (14:0, 18:1 and 20:4) into triacylglycerols (TG). Cells prelabeled with [ 14C]arachi...
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| Veröffentlicht in: | Life sciences (1973) 1993, Vol.52 (19), p.1567-1575 |
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| container_title | Life sciences (1973) |
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| creator | Tsai, S.S. Sun, A.Y. Kim, H.D. Sun, G.Y. |
| description | Exposure of PC-12 cells to MnCl
2 (10–100 μM for 1 to 3 days) resulted in both morphological and metabolic changes as indicated by promotion of neurite outgrowth and an increased incorporation of labeled fatty acids (14:0, 18:1 and 20:4) into triacylglycerols (TG). Cells prelabeled with [
14C]arachidonic acid (AA) showed a time-dependent decrease in labeled TG, and the rate of decline was inhibited by Mn (100 μM). In the pulse-chase type of study, the decrease in the proportion of labeled TG as well as labeled phosphatidylcholine (PC) was marked by an increase in labeled phosphatidylinositol and phosphatidylethanolamine. These results support previous notion that TG plays an important role in channeling the fatty acid nutrients for biosynthesis of membrane phospholipids. Thus, the Mn-induced inhibition of the ability to transfer TG fatty acids to other lipids in PC-12 cells may be an underlying cause for the observed morphological changes. |
| doi_str_mv | 10.1016/0024-3205(93)90057-A |
| format | Article |
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2 (10–100 μM for 1 to 3 days) resulted in both morphological and metabolic changes as indicated by promotion of neurite outgrowth and an increased incorporation of labeled fatty acids (14:0, 18:1 and 20:4) into triacylglycerols (TG). Cells prelabeled with [
14C]arachidonic acid (AA) showed a time-dependent decrease in labeled TG, and the rate of decline was inhibited by Mn (100 μM). In the pulse-chase type of study, the decrease in the proportion of labeled TG as well as labeled phosphatidylcholine (PC) was marked by an increase in labeled phosphatidylinositol and phosphatidylethanolamine. These results support previous notion that TG plays an important role in channeling the fatty acid nutrients for biosynthesis of membrane phospholipids. Thus, the Mn-induced inhibition of the ability to transfer TG fatty acids to other lipids in PC-12 cells may be an underlying cause for the observed morphological changes.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(93)90057-A</identifier><identifier>PMID: 8483384</identifier><identifier>CODEN: LIFSAK</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Arachidonic Acid - metabolism ; Arachidonic Acid - pharmacokinetics ; Biological and medical sciences ; Carbon Radioisotopes ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Chemical and industrial products toxicology. Toxic occupational diseases ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Glycolipids - metabolism ; Manganese - pharmacology ; Medical sciences ; Metals and various inorganic compounds ; Neurites - drug effects ; PC12 Cells - drug effects ; Rats ; Toxicology ; Triglycerides - metabolism</subject><ispartof>Life sciences (1973), 1993, Vol.52 (19), p.1567-1575</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-4157aeb627cbdad57ab113959129edc5abe0f3247be69ae853c9d34399ecbcfb3</citedby><cites>FETCH-LOGICAL-c417t-4157aeb627cbdad57ab113959129edc5abe0f3247be69ae853c9d34399ecbcfb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0024-3205(93)90057-A$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,4011,27905,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4885704$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8483384$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsai, S.S.</creatorcontrib><creatorcontrib>Sun, A.Y.</creatorcontrib><creatorcontrib>Kim, H.D.</creatorcontrib><creatorcontrib>Sun, G.Y.</creatorcontrib><title>Manganese exposure to PC-12 cells alters triacylglycerol metabolism and promotes neurite outgrowth</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Exposure of PC-12 cells to MnCl
2 (10–100 μM for 1 to 3 days) resulted in both morphological and metabolic changes as indicated by promotion of neurite outgrowth and an increased incorporation of labeled fatty acids (14:0, 18:1 and 20:4) into triacylglycerols (TG). Cells prelabeled with [
14C]arachidonic acid (AA) showed a time-dependent decrease in labeled TG, and the rate of decline was inhibited by Mn (100 μM). In the pulse-chase type of study, the decrease in the proportion of labeled TG as well as labeled phosphatidylcholine (PC) was marked by an increase in labeled phosphatidylinositol and phosphatidylethanolamine. These results support previous notion that TG plays an important role in channeling the fatty acid nutrients for biosynthesis of membrane phospholipids. Thus, the Mn-induced inhibition of the ability to transfer TG fatty acids to other lipids in PC-12 cells may be an underlying cause for the observed morphological changes.</description><subject>Animals</subject><subject>Arachidonic Acid - metabolism</subject><subject>Arachidonic Acid - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Carbon Radioisotopes</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Glycolipids - metabolism</subject><subject>Manganese - pharmacology</subject><subject>Medical sciences</subject><subject>Metals and various inorganic compounds</subject><subject>Neurites - drug effects</subject><subject>PC12 Cells - drug effects</subject><subject>Rats</subject><subject>Toxicology</subject><subject>Triglycerides - metabolism</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKsvCPwDJB1TRQ8CO7Ti-IK1W0CIVwQHOlu1MFiMnXmyHdv89CbvaY3sajeabp3lvEHpNyXtKaPOBkJpXrCbinWJXihAhq80TtKKtVBVpGH2KVmfkOXqR828yQ0KyC3TR8paxlq-Q_WrGnRkhA4b7fcxTAlwi_r6taI0dhJCxCQVSxiV54w5hFw4OUgx4gGJsDD4P2Iwd3qc4xAIZjzAlXwDHqexSvCu_XqJnvQkZXp3qGv38_OnH9qa6_Xb9Zbu5rRynslScCmnANrV0tjPd3FhKmRKK1go6J4wF0rOaSwuNMtAK5lTHOFMKnHW9ZWt0edSdT_kzQS568HmxMNuLU9ZSSNpILh8FaSMYYbP0GvEj6FLMOUGv98kPJh00JXr5gV4C1kvAWjH9_wd6M6-9OelPdoDuvHQKfZ6_Pc1Ndib0yYzO5zPG21ZIsmAfjxjMof31kHR2HkYHnU_giu6if_iOf8xCpC4</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Tsai, S.S.</creator><creator>Sun, A.Y.</creator><creator>Kim, H.D.</creator><creator>Sun, G.Y.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Manganese exposure to PC-12 cells alters triacylglycerol metabolism and promotes neurite outgrowth</title><author>Tsai, S.S. ; Sun, A.Y. ; Kim, H.D. ; Sun, G.Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-4157aeb627cbdad57ab113959129edc5abe0f3247be69ae853c9d34399ecbcfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Arachidonic Acid - metabolism</topic><topic>Arachidonic Acid - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Carbon Radioisotopes</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Glycolipids - metabolism</topic><topic>Manganese - pharmacology</topic><topic>Medical sciences</topic><topic>Metals and various inorganic compounds</topic><topic>Neurites - drug effects</topic><topic>PC12 Cells - drug effects</topic><topic>Rats</topic><topic>Toxicology</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, S.S.</creatorcontrib><creatorcontrib>Sun, A.Y.</creatorcontrib><creatorcontrib>Kim, H.D.</creatorcontrib><creatorcontrib>Sun, G.Y.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsai, S.S.</au><au>Sun, A.Y.</au><au>Kim, H.D.</au><au>Sun, G.Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Manganese exposure to PC-12 cells alters triacylglycerol metabolism and promotes neurite outgrowth</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1993</date><risdate>1993</risdate><volume>52</volume><issue>19</issue><spage>1567</spage><epage>1575</epage><pages>1567-1575</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><coden>LIFSAK</coden><abstract>Exposure of PC-12 cells to MnCl
2 (10–100 μM for 1 to 3 days) resulted in both morphological and metabolic changes as indicated by promotion of neurite outgrowth and an increased incorporation of labeled fatty acids (14:0, 18:1 and 20:4) into triacylglycerols (TG). Cells prelabeled with [
14C]arachidonic acid (AA) showed a time-dependent decrease in labeled TG, and the rate of decline was inhibited by Mn (100 μM). In the pulse-chase type of study, the decrease in the proportion of labeled TG as well as labeled phosphatidylcholine (PC) was marked by an increase in labeled phosphatidylinositol and phosphatidylethanolamine. These results support previous notion that TG plays an important role in channeling the fatty acid nutrients for biosynthesis of membrane phospholipids. Thus, the Mn-induced inhibition of the ability to transfer TG fatty acids to other lipids in PC-12 cells may be an underlying cause for the observed morphological changes.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>8483384</pmid><doi>10.1016/0024-3205(93)90057-A</doi><tpages>9</tpages></addata></record> |
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| ispartof | Life sciences (1973), 1993, Vol.52 (19), p.1567-1575 |
| issn | 0024-3205 1879-0631 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Arachidonic Acid - metabolism Arachidonic Acid - pharmacokinetics Biological and medical sciences Carbon Radioisotopes Cell Membrane - drug effects Cell Membrane - metabolism Chemical and industrial products toxicology. Toxic occupational diseases Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Glycolipids - metabolism Manganese - pharmacology Medical sciences Metals and various inorganic compounds Neurites - drug effects PC12 Cells - drug effects Rats Toxicology Triglycerides - metabolism |
| title | Manganese exposure to PC-12 cells alters triacylglycerol metabolism and promotes neurite outgrowth |
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