Possible role of arachidonic acid in the regulation of lactate production in rat Sertoli cells
Summary The aim of the study was to determine whether arachidonic acid (AA) is involved in the regulation of Sertoli cell lactate production and if this fatty acid participates in follicle‐stimulating hormone (FSH) regulation of Sertoli cell function. In a first set of experiments the effect of AA a...
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| Veröffentlicht in: | International journal of andrology 2003-10, Vol.26 (5), p.310-317 |
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| creator | Meroni, Silvina B. Riera, María F. Pellizzari, Eliana H. Schteingart, Helena F. Cigorraga, Selva B. |
| description | Summary
The aim of the study was to determine whether arachidonic acid (AA) is involved in the regulation of Sertoli cell lactate production and if this fatty acid participates in follicle‐stimulating hormone (FSH) regulation of Sertoli cell function. In a first set of experiments the effect of AA and porcine pancreas phospholipase A2 (PLA2) on lactate production, glucose uptake, lactate dehydrogenase (LDH) activity and LDH A mRNA levels in Sertoli cell cultures obtained from 20‐day‐old rats was evaluated. In a second set of experiments the effect of two PLA2 inhibitors – quinacrine (Q) and AACOCF3 – on FSH stimulation of the above‐mentioned parameters of Sertoli cell function was investigated. Treatment with PLA2 and AA increased Sertoli cell lactate production. The observed action of exogenously added PLA2 involved its catalytic properties responsible for AA release. PLA2 and AA treatments also stimulated Sertoli cell glucose uptake, LDH activity and LDH A mRNA levels. In order to determine whether AA participates in FSH regulation of Sertoli cell lactate production cells were incubated with FSH in the absence or presence of the PLA2 inhibitors Q and AACOCF3. Both drugs partially inhibited the ability of FSH to stimulate lactate production, glucose uptake and LDH activity. The present investigation suggests that AA is involved in the regulation of lactate production, glucose transport, LDH activity and LDH A mRNA levels. In addition, these results suggest that cytosolic PLA2 and AA may participate in FSH‐regulation of Sertoli cell energetic metabolism. |
| doi_str_mv | 10.1046/j.1365-2605.2003.00432.x |
| format | Article |
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The aim of the study was to determine whether arachidonic acid (AA) is involved in the regulation of Sertoli cell lactate production and if this fatty acid participates in follicle‐stimulating hormone (FSH) regulation of Sertoli cell function. In a first set of experiments the effect of AA and porcine pancreas phospholipase A2 (PLA2) on lactate production, glucose uptake, lactate dehydrogenase (LDH) activity and LDH A mRNA levels in Sertoli cell cultures obtained from 20‐day‐old rats was evaluated. In a second set of experiments the effect of two PLA2 inhibitors – quinacrine (Q) and AACOCF3 – on FSH stimulation of the above‐mentioned parameters of Sertoli cell function was investigated. Treatment with PLA2 and AA increased Sertoli cell lactate production. The observed action of exogenously added PLA2 involved its catalytic properties responsible for AA release. PLA2 and AA treatments also stimulated Sertoli cell glucose uptake, LDH activity and LDH A mRNA levels. In order to determine whether AA participates in FSH regulation of Sertoli cell lactate production cells were incubated with FSH in the absence or presence of the PLA2 inhibitors Q and AACOCF3. Both drugs partially inhibited the ability of FSH to stimulate lactate production, glucose uptake and LDH activity. The present investigation suggests that AA is involved in the regulation of lactate production, glucose transport, LDH activity and LDH A mRNA levels. In addition, these results suggest that cytosolic PLA2 and AA may participate in FSH‐regulation of Sertoli cell energetic metabolism.</description><identifier>ISSN: 0105-6263</identifier><identifier>EISSN: 1365-2605</identifier><identifier>DOI: 10.1046/j.1365-2605.2003.00432.x</identifier><identifier>PMID: 14511220</identifier><identifier>CODEN: IJANDP</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; arachidonic acid ; Arachidonic Acid - pharmacology ; Arachidonic Acid - physiology ; Biological and medical sciences ; Cells, Cultured ; Follicle Stimulating Hormone - pharmacology ; Follicle Stimulating Hormone - physiology ; follicle-stimulating hormone ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Gynecology. Andrology. Obstetrics ; L-Lactate Dehydrogenase - metabolism ; lactate ; Lactic Acid - biosynthesis ; Male ; Male genital diseases ; Mammalian male genital system ; Medical sciences ; phospholipase A2 ; Phospholipases A - metabolism ; Phospholipases A - pharmacology ; Phospholipases A2 ; Rats ; Rats, Sprague-Dawley ; Sertoli cells ; Sertoli Cells - metabolism ; Swine ; testis ; Vertebrates: reproduction</subject><ispartof>International journal of andrology, 2003-10, Vol.26 (5), p.310-317</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4992-c9b129b6603b7494d6d0268de2f853bccf985e6520ee3d665ad7ca41e6055b8f3</citedby><cites>FETCH-LOGICAL-c4992-c9b129b6603b7494d6d0268de2f853bccf985e6520ee3d665ad7ca41e6055b8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2605.2003.00432.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2605.2003.00432.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,1428,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15128555$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14511220$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meroni, Silvina B.</creatorcontrib><creatorcontrib>Riera, María F.</creatorcontrib><creatorcontrib>Pellizzari, Eliana H.</creatorcontrib><creatorcontrib>Schteingart, Helena F.</creatorcontrib><creatorcontrib>Cigorraga, Selva B.</creatorcontrib><title>Possible role of arachidonic acid in the regulation of lactate production in rat Sertoli cells</title><title>International journal of andrology</title><addtitle>Int J Androl</addtitle><description>Summary
The aim of the study was to determine whether arachidonic acid (AA) is involved in the regulation of Sertoli cell lactate production and if this fatty acid participates in follicle‐stimulating hormone (FSH) regulation of Sertoli cell function. In a first set of experiments the effect of AA and porcine pancreas phospholipase A2 (PLA2) on lactate production, glucose uptake, lactate dehydrogenase (LDH) activity and LDH A mRNA levels in Sertoli cell cultures obtained from 20‐day‐old rats was evaluated. In a second set of experiments the effect of two PLA2 inhibitors – quinacrine (Q) and AACOCF3 – on FSH stimulation of the above‐mentioned parameters of Sertoli cell function was investigated. Treatment with PLA2 and AA increased Sertoli cell lactate production. The observed action of exogenously added PLA2 involved its catalytic properties responsible for AA release. PLA2 and AA treatments also stimulated Sertoli cell glucose uptake, LDH activity and LDH A mRNA levels. In order to determine whether AA participates in FSH regulation of Sertoli cell lactate production cells were incubated with FSH in the absence or presence of the PLA2 inhibitors Q and AACOCF3. Both drugs partially inhibited the ability of FSH to stimulate lactate production, glucose uptake and LDH activity. The present investigation suggests that AA is involved in the regulation of lactate production, glucose transport, LDH activity and LDH A mRNA levels. In addition, these results suggest that cytosolic PLA2 and AA may participate in FSH‐regulation of Sertoli cell energetic metabolism.</description><subject>Animals</subject><subject>arachidonic acid</subject><subject>Arachidonic Acid - pharmacology</subject><subject>Arachidonic Acid - physiology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Follicle Stimulating Hormone - physiology</subject><subject>follicle-stimulating hormone</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>lactate</subject><subject>Lactic Acid - biosynthesis</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Mammalian male genital system</subject><subject>Medical sciences</subject><subject>phospholipase A2</subject><subject>Phospholipases A - metabolism</subject><subject>Phospholipases A - pharmacology</subject><subject>Phospholipases A2</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sertoli cells</subject><subject>Sertoli Cells - metabolism</subject><subject>Swine</subject><subject>testis</subject><subject>Vertebrates: reproduction</subject><issn>0105-6263</issn><issn>1365-2605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtP3DAUha2KqgzQv4C8gV2CH7GTLFggXqVCFNRWsMJy7Bvw4EnATtTh3-MwI9h2Y1u-37n33IMQpiSnpJAH85xyKTImicgZITwnpOAsX35Bs4_CBpoRSkQmmeSbaCvGOUlkxek3tEkLQSljZIbur_sYXeMBhz4dfYt10ObR2b5zBmvjLHYdHh5THR5GrwfXdxPltRn0APg59HY077-JC3rAvyEMvXfYgPdxB31ttY_wfX1vo79np3-Of2SXv84vjo8uM1PUNctM3VBWN1IS3pRFXVhpCZOVBdZWgjfGtHUlQApGALiVUmhbGl1QSHuKpmr5Ntpf9U1-XkaIg1q4ODnQHfRjVKUoaWooElitQBPS4gFa9RzcQodXRYmaslVzNUWopgjVlK16z1Ytk3R3PWNsFmA_heswE7C3BnQ02rdBd8bFT05QVgkxeThccf-ch9f_NqAufh5dpVfSZyu9iwMsP_Q6PClZ8lKo26tzVd_cndwWd1Td8DefIKP8</recordid><startdate>200310</startdate><enddate>200310</enddate><creator>Meroni, Silvina B.</creator><creator>Riera, María F.</creator><creator>Pellizzari, Eliana H.</creator><creator>Schteingart, Helena F.</creator><creator>Cigorraga, Selva B.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200310</creationdate><title>Possible role of arachidonic acid in the regulation of lactate production in rat Sertoli cells</title><author>Meroni, Silvina B. ; Riera, María F. ; Pellizzari, Eliana H. ; Schteingart, Helena F. ; Cigorraga, Selva B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4992-c9b129b6603b7494d6d0268de2f853bccf985e6520ee3d665ad7ca41e6055b8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>arachidonic acid</topic><topic>Arachidonic Acid - pharmacology</topic><topic>Arachidonic Acid - physiology</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Follicle Stimulating Hormone - physiology</topic><topic>follicle-stimulating hormone</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>lactate</topic><topic>Lactic Acid - biosynthesis</topic><topic>Male</topic><topic>Male genital diseases</topic><topic>Mammalian male genital system</topic><topic>Medical sciences</topic><topic>phospholipase A2</topic><topic>Phospholipases A - metabolism</topic><topic>Phospholipases A - pharmacology</topic><topic>Phospholipases A2</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sertoli cells</topic><topic>Sertoli Cells - metabolism</topic><topic>Swine</topic><topic>testis</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meroni, Silvina B.</creatorcontrib><creatorcontrib>Riera, María F.</creatorcontrib><creatorcontrib>Pellizzari, Eliana H.</creatorcontrib><creatorcontrib>Schteingart, Helena F.</creatorcontrib><creatorcontrib>Cigorraga, Selva B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meroni, Silvina B.</au><au>Riera, María F.</au><au>Pellizzari, Eliana H.</au><au>Schteingart, Helena F.</au><au>Cigorraga, Selva B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Possible role of arachidonic acid in the regulation of lactate production in rat Sertoli cells</atitle><jtitle>International journal of andrology</jtitle><addtitle>Int J Androl</addtitle><date>2003-10</date><risdate>2003</risdate><volume>26</volume><issue>5</issue><spage>310</spage><epage>317</epage><pages>310-317</pages><issn>0105-6263</issn><eissn>1365-2605</eissn><coden>IJANDP</coden><abstract>Summary
The aim of the study was to determine whether arachidonic acid (AA) is involved in the regulation of Sertoli cell lactate production and if this fatty acid participates in follicle‐stimulating hormone (FSH) regulation of Sertoli cell function. In a first set of experiments the effect of AA and porcine pancreas phospholipase A2 (PLA2) on lactate production, glucose uptake, lactate dehydrogenase (LDH) activity and LDH A mRNA levels in Sertoli cell cultures obtained from 20‐day‐old rats was evaluated. In a second set of experiments the effect of two PLA2 inhibitors – quinacrine (Q) and AACOCF3 – on FSH stimulation of the above‐mentioned parameters of Sertoli cell function was investigated. Treatment with PLA2 and AA increased Sertoli cell lactate production. The observed action of exogenously added PLA2 involved its catalytic properties responsible for AA release. PLA2 and AA treatments also stimulated Sertoli cell glucose uptake, LDH activity and LDH A mRNA levels. In order to determine whether AA participates in FSH regulation of Sertoli cell lactate production cells were incubated with FSH in the absence or presence of the PLA2 inhibitors Q and AACOCF3. Both drugs partially inhibited the ability of FSH to stimulate lactate production, glucose uptake and LDH activity. The present investigation suggests that AA is involved in the regulation of lactate production, glucose transport, LDH activity and LDH A mRNA levels. In addition, these results suggest that cytosolic PLA2 and AA may participate in FSH‐regulation of Sertoli cell energetic metabolism.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>14511220</pmid><doi>10.1046/j.1365-2605.2003.00432.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals arachidonic acid Arachidonic Acid - pharmacology Arachidonic Acid - physiology Biological and medical sciences Cells, Cultured Follicle Stimulating Hormone - pharmacology Follicle Stimulating Hormone - physiology follicle-stimulating hormone Fundamental and applied biological sciences. Psychology Glucose - metabolism Gynecology. Andrology. Obstetrics L-Lactate Dehydrogenase - metabolism lactate Lactic Acid - biosynthesis Male Male genital diseases Mammalian male genital system Medical sciences phospholipase A2 Phospholipases A - metabolism Phospholipases A - pharmacology Phospholipases A2 Rats Rats, Sprague-Dawley Sertoli cells Sertoli Cells - metabolism Swine testis Vertebrates: reproduction |
| title | Possible role of arachidonic acid in the regulation of lactate production in rat Sertoli cells |
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