Human serum IgE‐mediated mast cell degranulation shows poor correlation to allergen‐specific IgE content
Background: Although allergen‐specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering. Objectives: Analyse the degranulation capacity of immunochemically...
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| Veröffentlicht in: | Allergy (Copenhagen) 2003-10, Vol.58 (10), p.1037-1043 |
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| creator | Marchand, F. Mecheri, S. Guilloux, L. Iannascoli, B. Weyer, A. Blank, U. |
| description | Background: Although allergen‐specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering.
Objectives: Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti‐IgE or allergen using a rat mast cell line (RBL) transfected with the α‐chain of the human high‐affinity IgE receptor (FcɛRI).
Methods: Purified IgE specific for 4‐hydroxy‐3nitrophenylacetyl, purified IgE of unknown specificity, and sera from allergic patients sensitive to Dermatophagoides pteronyssinus and Dactylis glomerata were assessed. Degranulation was measured by a β‐hexosaminidase release assay after anti‐IgE or allergen‐specific challenge.
Results: For purified monoclonal IgE a significant correlation (r = 0.97) was found between the proportion of bound allergen‐specific IgE and the strength of the degranulation response. In contrast, no correlation (r = 0.27) was detected after sensitization with serum IgE.
Conclusion: Our studies demonstrate that mast cell activation mediated through IgE from allergic patients is a result of complex relationships that are not only dependent on allergen‐specific IgE content but also relate to the capacity to efficiently sensitize and trigger the signalling responses that lead to degranulation. |
| doi_str_mv | 10.1034/j.1398-9995.2003.00251.x |
| format | Article |
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Objectives: Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti‐IgE or allergen using a rat mast cell line (RBL) transfected with the α‐chain of the human high‐affinity IgE receptor (FcɛRI).
Methods: Purified IgE specific for 4‐hydroxy‐3nitrophenylacetyl, purified IgE of unknown specificity, and sera from allergic patients sensitive to Dermatophagoides pteronyssinus and Dactylis glomerata were assessed. Degranulation was measured by a β‐hexosaminidase release assay after anti‐IgE or allergen‐specific challenge.
Results: For purified monoclonal IgE a significant correlation (r = 0.97) was found between the proportion of bound allergen‐specific IgE and the strength of the degranulation response. In contrast, no correlation (r = 0.27) was detected after sensitization with serum IgE.
Conclusion: Our studies demonstrate that mast cell activation mediated through IgE from allergic patients is a result of complex relationships that are not only dependent on allergen‐specific IgE content but also relate to the capacity to efficiently sensitize and trigger the signalling responses that lead to degranulation.</description><identifier>ISSN: 0105-4538</identifier><identifier>EISSN: 1398-9995</identifier><identifier>DOI: 10.1034/j.1398-9995.2003.00251.x</identifier><identifier>PMID: 14510723</identifier><identifier>CODEN: LLRGDY</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>allergenicity ; Allergens - immunology ; Allergic diseases ; Animals ; beta-N-Acetylhexosaminidases - metabolism ; Biological and medical sciences ; Cell Degranulation ; Cell Line ; Cells, Cultured ; Dactylis - immunology ; degranulation ; Dermatophagoides pteronyssinus - immunology ; Dose-Response Relationship, Immunologic ; General aspects ; hu FcɛRI ; Humans ; Hypersensitivity, Immediate - immunology ; IgE ; Immunoglobulin E - blood ; Immunoglobulin E - genetics ; Immunoglobulin E - immunology ; Immunopathology ; Mast Cells - immunology ; Medical sciences ; Rats ; RBL‐2H3 ; Transfection</subject><ispartof>Allergy (Copenhagen), 2003-10, Vol.58 (10), p.1037-1043</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4911-ac0afdae75453ebc5f9751a2af2e1c38138b7ed9e1b4513b01862caaee0666653</citedby><cites>FETCH-LOGICAL-c4911-ac0afdae75453ebc5f9751a2af2e1c38138b7ed9e1b4513b01862caaee0666653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1034%2Fj.1398-9995.2003.00251.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1398-9995.2003.00251.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15155088$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14510723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marchand, F.</creatorcontrib><creatorcontrib>Mecheri, S.</creatorcontrib><creatorcontrib>Guilloux, L.</creatorcontrib><creatorcontrib>Iannascoli, B.</creatorcontrib><creatorcontrib>Weyer, A.</creatorcontrib><creatorcontrib>Blank, U.</creatorcontrib><title>Human serum IgE‐mediated mast cell degranulation shows poor correlation to allergen‐specific IgE content</title><title>Allergy (Copenhagen)</title><addtitle>Allergy</addtitle><description>Background: Although allergen‐specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering.
Objectives: Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti‐IgE or allergen using a rat mast cell line (RBL) transfected with the α‐chain of the human high‐affinity IgE receptor (FcɛRI).
Methods: Purified IgE specific for 4‐hydroxy‐3nitrophenylacetyl, purified IgE of unknown specificity, and sera from allergic patients sensitive to Dermatophagoides pteronyssinus and Dactylis glomerata were assessed. Degranulation was measured by a β‐hexosaminidase release assay after anti‐IgE or allergen‐specific challenge.
Results: For purified monoclonal IgE a significant correlation (r = 0.97) was found between the proportion of bound allergen‐specific IgE and the strength of the degranulation response. In contrast, no correlation (r = 0.27) was detected after sensitization with serum IgE.
Conclusion: Our studies demonstrate that mast cell activation mediated through IgE from allergic patients is a result of complex relationships that are not only dependent on allergen‐specific IgE content but also relate to the capacity to efficiently sensitize and trigger the signalling responses that lead to degranulation.</description><subject>allergenicity</subject><subject>Allergens - immunology</subject><subject>Allergic diseases</subject><subject>Animals</subject><subject>beta-N-Acetylhexosaminidases - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Degranulation</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Dactylis - immunology</subject><subject>degranulation</subject><subject>Dermatophagoides pteronyssinus - immunology</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>General aspects</subject><subject>hu FcɛRI</subject><subject>Humans</subject><subject>Hypersensitivity, Immediate - immunology</subject><subject>IgE</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin E - genetics</subject><subject>Immunoglobulin E - immunology</subject><subject>Immunopathology</subject><subject>Mast Cells - immunology</subject><subject>Medical sciences</subject><subject>Rats</subject><subject>RBL‐2H3</subject><subject>Transfection</subject><issn>0105-4538</issn><issn>1398-9995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxi1ERZfCKyBf4JbgiePGlrhUVaGVVuqlPVsTZ7Jk5SSLnajtrY_AM_IkOGxEj-CLR_Zv_n0fYxxEDkKWn_c5SKMzY4zKCyFkLkShIH98xTZ_P16zjQChslJJfcrexrgXQlSFEW_YKZQKUiw3zF_PPQ48Uph7frO7-vX8s6emw4ka3mOcuCPveUO7gMPscerGBH8fHyI_jGPgbgyB1udp5Og9hR0NqUo8kOvazi1FEzZMNEzv2EmLPtL79T5j91-v7i6vs-3tt5vLi23mSgOQoRPYNkiVSrNT7VRrKgVYYFsQOKlB6rqixhDUaQ9ZC9DnhUMkEufpKHnGPh3rHsL4Y6Y42b6LyyI40DhHW6kKQMvynyBoo0ySNoH6CLowxhiotYfQ9RieLAi7WGL3dlHeLsrbxRL7xxL7mFI_rD3mOkn7krh6kICPK4DRoW-T0q6LL5wCpYTWifty5B46T0__PYC92G5TIH8DWGKqPQ</recordid><startdate>200310</startdate><enddate>200310</enddate><creator>Marchand, F.</creator><creator>Mecheri, S.</creator><creator>Guilloux, L.</creator><creator>Iannascoli, B.</creator><creator>Weyer, A.</creator><creator>Blank, U.</creator><general>Munksgaard International Publishers</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200310</creationdate><title>Human serum IgE‐mediated mast cell degranulation shows poor correlation to allergen‐specific IgE content</title><author>Marchand, F. ; Mecheri, S. ; Guilloux, L. ; Iannascoli, B. ; Weyer, A. ; Blank, U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4911-ac0afdae75453ebc5f9751a2af2e1c38138b7ed9e1b4513b01862caaee0666653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>allergenicity</topic><topic>Allergens - immunology</topic><topic>Allergic diseases</topic><topic>Animals</topic><topic>beta-N-Acetylhexosaminidases - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Degranulation</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Dactylis - immunology</topic><topic>degranulation</topic><topic>Dermatophagoides pteronyssinus - immunology</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>General aspects</topic><topic>hu FcɛRI</topic><topic>Humans</topic><topic>Hypersensitivity, Immediate - immunology</topic><topic>IgE</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin E - genetics</topic><topic>Immunoglobulin E - immunology</topic><topic>Immunopathology</topic><topic>Mast Cells - immunology</topic><topic>Medical sciences</topic><topic>Rats</topic><topic>RBL‐2H3</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marchand, F.</creatorcontrib><creatorcontrib>Mecheri, S.</creatorcontrib><creatorcontrib>Guilloux, L.</creatorcontrib><creatorcontrib>Iannascoli, B.</creatorcontrib><creatorcontrib>Weyer, A.</creatorcontrib><creatorcontrib>Blank, U.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Allergy (Copenhagen)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marchand, F.</au><au>Mecheri, S.</au><au>Guilloux, L.</au><au>Iannascoli, B.</au><au>Weyer, A.</au><au>Blank, U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human serum IgE‐mediated mast cell degranulation shows poor correlation to allergen‐specific IgE content</atitle><jtitle>Allergy (Copenhagen)</jtitle><addtitle>Allergy</addtitle><date>2003-10</date><risdate>2003</risdate><volume>58</volume><issue>10</issue><spage>1037</spage><epage>1043</epage><pages>1037-1043</pages><issn>0105-4538</issn><eissn>1398-9995</eissn><coden>LLRGDY</coden><abstract>Background: Although allergen‐specific IgE content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering.
Objectives: Analyse the degranulation capacity of immunochemically defined purified and serum IgE after challenge with anti‐IgE or allergen using a rat mast cell line (RBL) transfected with the α‐chain of the human high‐affinity IgE receptor (FcɛRI).
Methods: Purified IgE specific for 4‐hydroxy‐3nitrophenylacetyl, purified IgE of unknown specificity, and sera from allergic patients sensitive to Dermatophagoides pteronyssinus and Dactylis glomerata were assessed. Degranulation was measured by a β‐hexosaminidase release assay after anti‐IgE or allergen‐specific challenge.
Results: For purified monoclonal IgE a significant correlation (r = 0.97) was found between the proportion of bound allergen‐specific IgE and the strength of the degranulation response. In contrast, no correlation (r = 0.27) was detected after sensitization with serum IgE.
Conclusion: Our studies demonstrate that mast cell activation mediated through IgE from allergic patients is a result of complex relationships that are not only dependent on allergen‐specific IgE content but also relate to the capacity to efficiently sensitize and trigger the signalling responses that lead to degranulation.</abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>14510723</pmid><doi>10.1034/j.1398-9995.2003.00251.x</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | allergenicity Allergens - immunology Allergic diseases Animals beta-N-Acetylhexosaminidases - metabolism Biological and medical sciences Cell Degranulation Cell Line Cells, Cultured Dactylis - immunology degranulation Dermatophagoides pteronyssinus - immunology Dose-Response Relationship, Immunologic General aspects hu FcɛRI Humans Hypersensitivity, Immediate - immunology IgE Immunoglobulin E - blood Immunoglobulin E - genetics Immunoglobulin E - immunology Immunopathology Mast Cells - immunology Medical sciences Rats RBL‐2H3 Transfection |
| title | Human serum IgE‐mediated mast cell degranulation shows poor correlation to allergen‐specific IgE content |
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