Nuclear benzodiazepine binding : possible interaction with thyroid hormone receptors
The biochemical and pharmacological properties of nuclear [3H]flunitrazepam in brain tissues were studied. Nuclear [3H]flunitrazepam binding is saturable for both central and peripheral binding sites. Inosine and hypoxanthine displace nuclear [3H]flunitrazepam binding with greater potency than the m...
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| Veröffentlicht in: | Neurochemical research 1993-03, Vol.18 (3), p.305-311 |
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| creator | DALEZIOS, Y MATSOKIS, N |
| description | The biochemical and pharmacological properties of nuclear [3H]flunitrazepam in brain tissues were studied. Nuclear [3H]flunitrazepam binding is saturable for both central and peripheral binding sites. Inosine and hypoxanthine displace nuclear [3H]flunitrazepam binding with greater potency than the membrane [3H]flunitrazepam binding. Triiodothyronine (T3) increases the maximum number of binding sites (Bmax) of nuclear [3H]flunitrazepam binding in vitro while thyroxine (T4) does not have any effect. Diazepam reduces the affinity of nuclear 125I-T3 binding in vitro, while the Bmax is not affected significantly. Mild digestion of chromatin, using micrococcal nuclease, reveals that a major portion of nuclear [3H]flunitrazepam binding sites are located on chromatin. These data suggest a functional role for nuclear benzodiazepine binding and a possible modulatory effect of benzodiazepines on T3 binding with its nuclear receptors. |
| doi_str_mv | 10.1007/bf00969087 |
| format | Article |
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Nuclear [3H]flunitrazepam binding is saturable for both central and peripheral binding sites. Inosine and hypoxanthine displace nuclear [3H]flunitrazepam binding with greater potency than the membrane [3H]flunitrazepam binding. Triiodothyronine (T3) increases the maximum number of binding sites (Bmax) of nuclear [3H]flunitrazepam binding in vitro while thyroxine (T4) does not have any effect. Diazepam reduces the affinity of nuclear 125I-T3 binding in vitro, while the Bmax is not affected significantly. Mild digestion of chromatin, using micrococcal nuclease, reveals that a major portion of nuclear [3H]flunitrazepam binding sites are located on chromatin. These data suggest a functional role for nuclear benzodiazepine binding and a possible modulatory effect of benzodiazepines on T3 binding with its nuclear receptors.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/bf00969087</identifier><identifier>PMID: 8479599</identifier><identifier>CODEN: NEREDZ</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animals ; Benzodiazepines - metabolism ; Biological and medical sciences ; Brain - metabolism ; Cell Nucleus - metabolism ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; DNA - metabolism ; Flunitrazepam - metabolism ; Fundamental and applied biological sciences. Psychology ; Hypoxanthine ; Hypoxanthines - metabolism ; Inosine - metabolism ; Rats ; Rats, Wistar ; Receptors, Thyroid Hormone - metabolism ; Thyroxine - pharmacology ; Triiodothyronine - metabolism ; Triiodothyronine - pharmacology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neurochemical research, 1993-03, Vol.18 (3), p.305-311</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-dc2b67bd5feeac8c4ba9ec371b9089c62845758df0e55ed0531b43f4682cf31d3</citedby><cites>FETCH-LOGICAL-c371t-dc2b67bd5feeac8c4ba9ec371b9089c62845758df0e55ed0531b43f4682cf31d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4663649$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8479599$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DALEZIOS, Y</creatorcontrib><creatorcontrib>MATSOKIS, N</creatorcontrib><title>Nuclear benzodiazepine binding : possible interaction with thyroid hormone receptors</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>The biochemical and pharmacological properties of nuclear [3H]flunitrazepam in brain tissues were studied. Nuclear [3H]flunitrazepam binding is saturable for both central and peripheral binding sites. Inosine and hypoxanthine displace nuclear [3H]flunitrazepam binding with greater potency than the membrane [3H]flunitrazepam binding. Triiodothyronine (T3) increases the maximum number of binding sites (Bmax) of nuclear [3H]flunitrazepam binding in vitro while thyroxine (T4) does not have any effect. Diazepam reduces the affinity of nuclear 125I-T3 binding in vitro, while the Bmax is not affected significantly. Mild digestion of chromatin, using micrococcal nuclease, reveals that a major portion of nuclear [3H]flunitrazepam binding sites are located on chromatin. These data suggest a functional role for nuclear benzodiazepine binding and a possible modulatory effect of benzodiazepines on T3 binding with its nuclear receptors.</description><subject>Animals</subject><subject>Benzodiazepines - metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>DNA - metabolism</subject><subject>Flunitrazepam - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypoxanthine</subject><subject>Hypoxanthines - metabolism</subject><subject>Inosine - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Thyroid Hormone - metabolism</subject><subject>Thyroxine - pharmacology</subject><subject>Triiodothyronine - metabolism</subject><subject>Triiodothyronine - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1PwzAQxS0EKqWwsCNlQAxIAbuO45gNKgpIFSxljvxxoUZJHOxUqP3rcUXoynQ6vd896b1D6JzgG4Ixv1UVxiIXuOAHaEwYp2lc6CEaY5pnKSUCH6OTED4xjviUjNCoyLhgQozR8nWta5A-UdBunbFyC51tIVG2Nbb9SO6SzoVgVQ2JbXvwUvfWtcm37VdJv9p4Z02ycr5x8caDhq53Ppyio0rWAc6GOUHv88fl7DldvD29zO4Xqaac9KnRU5VzZVgFIHWhMyUF7CQVowidT4uMcVaYCgNjYDCjRGW0yvJiqitKDJ2gq1_fzruvNYS-bGzQUNeyBbcOJWc8dsLJvyDJGSakYBG8_gW1j7E9VGXnbSP9piS43HVdPsz_uo7wxeC6Vg2YPTqUG_XLQZdBy7rystU27LEsz-N7BP0B4uWHKw</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>DALEZIOS, Y</creator><creator>MATSOKIS, N</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>Nuclear benzodiazepine binding : possible interaction with thyroid hormone receptors</title><author>DALEZIOS, Y ; MATSOKIS, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-dc2b67bd5feeac8c4ba9ec371b9089c62845758df0e55ed0531b43f4682cf31d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Benzodiazepines - metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>DNA - metabolism</topic><topic>Flunitrazepam - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypoxanthine</topic><topic>Hypoxanthines - metabolism</topic><topic>Inosine - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Thyroid Hormone - metabolism</topic><topic>Thyroxine - pharmacology</topic><topic>Triiodothyronine - metabolism</topic><topic>Triiodothyronine - pharmacology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DALEZIOS, Y</creatorcontrib><creatorcontrib>MATSOKIS, N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DALEZIOS, Y</au><au>MATSOKIS, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear benzodiazepine binding : possible interaction with thyroid hormone receptors</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>18</volume><issue>3</issue><spage>305</spage><epage>311</epage><pages>305-311</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>The biochemical and pharmacological properties of nuclear [3H]flunitrazepam in brain tissues were studied. Nuclear [3H]flunitrazepam binding is saturable for both central and peripheral binding sites. Inosine and hypoxanthine displace nuclear [3H]flunitrazepam binding with greater potency than the membrane [3H]flunitrazepam binding. Triiodothyronine (T3) increases the maximum number of binding sites (Bmax) of nuclear [3H]flunitrazepam binding in vitro while thyroxine (T4) does not have any effect. Diazepam reduces the affinity of nuclear 125I-T3 binding in vitro, while the Bmax is not affected significantly. Mild digestion of chromatin, using micrococcal nuclease, reveals that a major portion of nuclear [3H]flunitrazepam binding sites are located on chromatin. These data suggest a functional role for nuclear benzodiazepine binding and a possible modulatory effect of benzodiazepines on T3 binding with its nuclear receptors.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>8479599</pmid><doi>10.1007/bf00969087</doi><tpages>7</tpages></addata></record> |
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| ispartof | Neurochemical research, 1993-03, Vol.18 (3), p.305-311 |
| issn | 0364-3190 1573-6903 |
| language | eng |
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| subjects | Animals Benzodiazepines - metabolism Biological and medical sciences Brain - metabolism Cell Nucleus - metabolism Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors DNA - metabolism Flunitrazepam - metabolism Fundamental and applied biological sciences. Psychology Hypoxanthine Hypoxanthines - metabolism Inosine - metabolism Rats Rats, Wistar Receptors, Thyroid Hormone - metabolism Thyroxine - pharmacology Triiodothyronine - metabolism Triiodothyronine - pharmacology Vertebrates: nervous system and sense organs |
| title | Nuclear benzodiazepine binding : possible interaction with thyroid hormone receptors |
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