Molecular cloning, expression analysis and cellular localization of gomesin, an anti-microbial peptide from hemocytes of the spider Acanthoscurria gomesiana
Gomesin is a cationic anti-microbial peptide of 18 amino acid residues isolated from the hemocytes of unchallenged tarantula spider Acanthoscurria gomesiana. This paper reports the first study of the processing and cellular location of an anti-microbial peptide (AMP) in spiders. Gomesin cDNA sequenc...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2003-10, Vol.33 (10), p.1011-1016 |
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| container_title | Insect biochemistry and molecular biology |
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| creator | Lorenzini, Daniel M Fukuzawa, Aline H da Silva, Pedro I Machado-Santelli, Glaucia Bijovsky, Alcira Tania Daffre, Sirlei |
| description | Gomesin is a cationic anti-microbial peptide of 18 amino acid residues isolated from the hemocytes of unchallenged tarantula spider
Acanthoscurria gomesiana. This paper reports the first study of the processing and cellular location of an anti-microbial peptide (AMP) in spiders. Gomesin cDNA sequence analysis indicated that it is processed from a precursor containing a signal peptide (23 amino acid residues) and a negative C-terminal region (43 amino acid residues). The gomesin gene was constitutively transcribed in hemocytes and the gene product localized in hemocyte granules. The constitutive production of gomesin by a spider is discussed in the context of an ancient mechanism of AMP regulation and storage. |
| doi_str_mv | 10.1016/S0965-1748(03)00115-2 |
| format | Article |
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Acanthoscurria gomesiana. This paper reports the first study of the processing and cellular location of an anti-microbial peptide (AMP) in spiders. Gomesin cDNA sequence analysis indicated that it is processed from a precursor containing a signal peptide (23 amino acid residues) and a negative C-terminal region (43 amino acid residues). The gomesin gene was constitutively transcribed in hemocytes and the gene product localized in hemocyte granules. The constitutive production of gomesin by a spider is discussed in the context of an ancient mechanism of AMP regulation and storage.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/S0965-1748(03)00115-2</identifier><identifier>PMID: 14505694</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Anti-microbial peptides ; Antimicrobial Cationic Peptides - chemistry ; Antimicrobial Cationic Peptides - genetics ; Antimicrobial Cationic Peptides - metabolism ; antimicrobial peptides ; Araneae ; Base Sequence ; Blood Proteins - biosynthesis ; Blood Proteins - genetics ; Blood Proteins - metabolism ; Blood Proteins - pharmacology ; Cloning, Molecular ; complementary DNA ; DNA, Complementary - genetics ; gene expression ; Gomesin ; Hemocyte ; hemocytes ; Hemocytes - metabolism ; Immunocytochemistry ; Immunohistochemistry ; immunolocalization ; messenger RNA ; Microscopy, Confocal ; molecular cloning ; Molecular Sequence Data ; nucleotide sequences ; peptide processing ; post-translational modification ; Processing ; Protein Biosynthesis ; Protein Precursors - genetics ; Protein Precursors - metabolism ; Spider ; Spiders - metabolism ; structural genes ; tarantual spiders ; Theraphosidae ; Tissue Distribution ; Transcription, Genetic</subject><ispartof>Insect biochemistry and molecular biology, 2003-10, Vol.33 (10), p.1011-1016</ispartof><rights>2003 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-1824517901861489088eb9340e4f2a47e4cca426fd4d98e719bb13ccf9639a733</citedby><cites>FETCH-LOGICAL-c416t-1824517901861489088eb9340e4f2a47e4cca426fd4d98e719bb13ccf9639a733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0965-1748(03)00115-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14505694$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lorenzini, Daniel M</creatorcontrib><creatorcontrib>Fukuzawa, Aline H</creatorcontrib><creatorcontrib>da Silva, Pedro I</creatorcontrib><creatorcontrib>Machado-Santelli, Glaucia</creatorcontrib><creatorcontrib>Bijovsky, Alcira Tania</creatorcontrib><creatorcontrib>Daffre, Sirlei</creatorcontrib><title>Molecular cloning, expression analysis and cellular localization of gomesin, an anti-microbial peptide from hemocytes of the spider Acanthoscurria gomesiana</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>Gomesin is a cationic anti-microbial peptide of 18 amino acid residues isolated from the hemocytes of unchallenged tarantula spider
Acanthoscurria gomesiana. This paper reports the first study of the processing and cellular location of an anti-microbial peptide (AMP) in spiders. Gomesin cDNA sequence analysis indicated that it is processed from a precursor containing a signal peptide (23 amino acid residues) and a negative C-terminal region (43 amino acid residues). The gomesin gene was constitutively transcribed in hemocytes and the gene product localized in hemocyte granules. The constitutive production of gomesin by a spider is discussed in the context of an ancient mechanism of AMP regulation and storage.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Anti-microbial peptides</subject><subject>Antimicrobial Cationic Peptides - chemistry</subject><subject>Antimicrobial Cationic Peptides - genetics</subject><subject>Antimicrobial Cationic Peptides - metabolism</subject><subject>antimicrobial peptides</subject><subject>Araneae</subject><subject>Base Sequence</subject><subject>Blood Proteins - biosynthesis</subject><subject>Blood Proteins - genetics</subject><subject>Blood Proteins - metabolism</subject><subject>Blood Proteins - pharmacology</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>DNA, Complementary - genetics</subject><subject>gene expression</subject><subject>Gomesin</subject><subject>Hemocyte</subject><subject>hemocytes</subject><subject>Hemocytes - metabolism</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>immunolocalization</subject><subject>messenger RNA</subject><subject>Microscopy, Confocal</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>peptide processing</subject><subject>post-translational modification</subject><subject>Processing</subject><subject>Protein Biosynthesis</subject><subject>Protein Precursors - genetics</subject><subject>Protein Precursors - metabolism</subject><subject>Spider</subject><subject>Spiders - metabolism</subject><subject>structural genes</subject><subject>tarantual spiders</subject><subject>Theraphosidae</subject><subject>Tissue Distribution</subject><subject>Transcription, Genetic</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-K1DAUxoMo7rj6CGquRGGrJ23SJlfLsvgPVrxY9zqk6elMJG1q0orjs_iwpjODXi4ETiC_73w55yPkOYO3DFj97hZULQrWcPkaqjcAjImifEA2TDaqgJLDQ7L5h5yRJyl9BwDORfOYnDEuQNSKb8ifL8GjXbyJ1PowunF7QfHXFDElF0ZqRuP3yaV86ahF7w-kD9Z499vMKxJ6ug0DJjdeZCqf2RWDszG0zng64TS7Dmkfw0B3OAS7nzGtonmHNE35LdIrm1W7kOwSozOndtn6KXnUG5_w2amek7sP779dfypuvn78fH11U1jO6rlgsuSCNQqYrBmXCqTEVlUckPel4Q1yaw0v677jnZLYMNW2rLK2V3WlTFNV5-TVse8Uw48F06wHl9ZpzYhhSboRDdSgyntBJqWqRCkyKI5g3kNKEXs9RTeYuNcM9JqfPuSn13A0VPqQn14NXpwMlnbA7r_qFFgGXh6B3gRtttElfXdbAquAQak4W4nLI4F5Yz8dRp2sw9Fi5yLaWXfB3fOJv5d1ti8</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Lorenzini, Daniel M</creator><creator>Fukuzawa, Aline H</creator><creator>da Silva, Pedro I</creator><creator>Machado-Santelli, Glaucia</creator><creator>Bijovsky, Alcira Tania</creator><creator>Daffre, Sirlei</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Molecular cloning, expression analysis and cellular localization of gomesin, an anti-microbial peptide from hemocytes of the spider Acanthoscurria gomesiana</title><author>Lorenzini, Daniel M ; Fukuzawa, Aline H ; da Silva, Pedro I ; Machado-Santelli, Glaucia ; Bijovsky, Alcira Tania ; Daffre, Sirlei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-1824517901861489088eb9340e4f2a47e4cca426fd4d98e719bb13ccf9639a733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Anti-microbial peptides</topic><topic>Antimicrobial Cationic Peptides - chemistry</topic><topic>Antimicrobial Cationic Peptides - genetics</topic><topic>Antimicrobial Cationic Peptides - metabolism</topic><topic>antimicrobial peptides</topic><topic>Araneae</topic><topic>Base Sequence</topic><topic>Blood Proteins - biosynthesis</topic><topic>Blood Proteins - genetics</topic><topic>Blood Proteins - metabolism</topic><topic>Blood Proteins - pharmacology</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>DNA, Complementary - genetics</topic><topic>gene expression</topic><topic>Gomesin</topic><topic>Hemocyte</topic><topic>hemocytes</topic><topic>Hemocytes - metabolism</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>immunolocalization</topic><topic>messenger RNA</topic><topic>Microscopy, Confocal</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>peptide processing</topic><topic>post-translational modification</topic><topic>Processing</topic><topic>Protein Biosynthesis</topic><topic>Protein Precursors - genetics</topic><topic>Protein Precursors - metabolism</topic><topic>Spider</topic><topic>Spiders - metabolism</topic><topic>structural genes</topic><topic>tarantual spiders</topic><topic>Theraphosidae</topic><topic>Tissue Distribution</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lorenzini, Daniel M</creatorcontrib><creatorcontrib>Fukuzawa, Aline H</creatorcontrib><creatorcontrib>da Silva, Pedro I</creatorcontrib><creatorcontrib>Machado-Santelli, Glaucia</creatorcontrib><creatorcontrib>Bijovsky, Alcira Tania</creatorcontrib><creatorcontrib>Daffre, Sirlei</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lorenzini, Daniel M</au><au>Fukuzawa, Aline H</au><au>da Silva, Pedro I</au><au>Machado-Santelli, Glaucia</au><au>Bijovsky, Alcira Tania</au><au>Daffre, Sirlei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning, expression analysis and cellular localization of gomesin, an anti-microbial peptide from hemocytes of the spider Acanthoscurria gomesiana</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>33</volume><issue>10</issue><spage>1011</spage><epage>1016</epage><pages>1011-1016</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Gomesin is a cationic anti-microbial peptide of 18 amino acid residues isolated from the hemocytes of unchallenged tarantula spider
Acanthoscurria gomesiana. This paper reports the first study of the processing and cellular location of an anti-microbial peptide (AMP) in spiders. Gomesin cDNA sequence analysis indicated that it is processed from a precursor containing a signal peptide (23 amino acid residues) and a negative C-terminal region (43 amino acid residues). The gomesin gene was constitutively transcribed in hemocytes and the gene product localized in hemocyte granules. The constitutive production of gomesin by a spider is discussed in the context of an ancient mechanism of AMP regulation and storage.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>14505694</pmid><doi>10.1016/S0965-1748(03)00115-2</doi><tpages>6</tpages></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Animals Anti-microbial peptides Antimicrobial Cationic Peptides - chemistry Antimicrobial Cationic Peptides - genetics Antimicrobial Cationic Peptides - metabolism antimicrobial peptides Araneae Base Sequence Blood Proteins - biosynthesis Blood Proteins - genetics Blood Proteins - metabolism Blood Proteins - pharmacology Cloning, Molecular complementary DNA DNA, Complementary - genetics gene expression Gomesin Hemocyte hemocytes Hemocytes - metabolism Immunocytochemistry Immunohistochemistry immunolocalization messenger RNA Microscopy, Confocal molecular cloning Molecular Sequence Data nucleotide sequences peptide processing post-translational modification Processing Protein Biosynthesis Protein Precursors - genetics Protein Precursors - metabolism Spider Spiders - metabolism structural genes tarantual spiders Theraphosidae Tissue Distribution Transcription, Genetic |
| title | Molecular cloning, expression analysis and cellular localization of gomesin, an anti-microbial peptide from hemocytes of the spider Acanthoscurria gomesiana |
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