Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells
In order to examine the effects of the overexpression of c-erbB-2 (HER-2, neu) on human bronchial epithelial cells, a human c-erbB-2 expression vector was introduced into the simian virus 40 large T-antigen-immortalized human bronchial epithelial cell line BEAS-2B. Isolation of multiple clonal cell...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1993-05, Vol.53 (9), p.2035-2043 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | NOGUCHI, M MURAKAMI, M BENNETT, W LUPU, R HUI, F. JR HARRIS, C. C GERWIN, B. I |
| description | In order to examine the effects of the overexpression of c-erbB-2 (HER-2, neu) on human bronchial epithelial cells, a human c-erbB-2 expression vector was introduced into the simian virus 40 large T-antigen-immortalized human bronchial epithelial cell line BEAS-2B. Isolation of multiple clonal cell lines after selection revealed a wide range of expression of the gene product gp185erbB-2. While three of six clones tested expressed gp185erbB-2 at levels detectable by immunocytochemistry, only one, B2BE6, induced adenocarcinoma-like tumors in athymic nude mice. Both a nontumorigenic clone, B2BE2, and a tumorigenic clone, B2BE6, expressed comparable amounts of gp185erbB-2, which became phosphorylated on tyrosine in response to treatment with the c-erbB-2 ligands gp30 and p75. These data suggest that overexpression of c-erbB-2 in human bronchial epithelial cells can contribute to, but is not sufficient for, induction of tumorigenicity in this human model system. |
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JR ; HARRIS, C. C ; GERWIN, B. I</creator><creatorcontrib>NOGUCHI, M ; MURAKAMI, M ; BENNETT, W ; LUPU, R ; HUI, F. JR ; HARRIS, C. C ; GERWIN, B. I</creatorcontrib><description>In order to examine the effects of the overexpression of c-erbB-2 (HER-2, neu) on human bronchial epithelial cells, a human c-erbB-2 expression vector was introduced into the simian virus 40 large T-antigen-immortalized human bronchial epithelial cell line BEAS-2B. Isolation of multiple clonal cell lines after selection revealed a wide range of expression of the gene product gp185erbB-2. While three of six clones tested expressed gp185erbB-2 at levels detectable by immunocytochemistry, only one, B2BE6, induced adenocarcinoma-like tumors in athymic nude mice. Both a nontumorigenic clone, B2BE2, and a tumorigenic clone, B2BE6, expressed comparable amounts of gp185erbB-2, which became phosphorylated on tyrosine in response to treatment with the c-erbB-2 ligands gp30 and p75. These data suggest that overexpression of c-erbB-2 in human bronchial epithelial cells can contribute to, but is not sufficient for, induction of tumorigenicity in this human model system.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 7683250</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Western ; Bronchi - metabolism ; Cell Division ; Cell Line ; Epidermal Growth Factor - genetics ; Epithelium - metabolism ; Gene Expression ; Humans ; In Vitro Techniques ; Ki-67 Antigen ; Ligands ; Medical sciences ; Mice ; Mice, Nude ; Neoplasm Proteins - metabolism ; Neoplasms, Experimental - genetics ; Nuclear Proteins - metabolism ; Phosphorylation ; Phosphotyrosine ; Pneumology ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Receptor, ErbB-2 ; RNA, Messenger - genetics ; Transfection ; Transforming Growth Factor alpha - genetics ; Tumors of the respiratory system and mediastinum ; Tyrosine - analogs & derivatives ; Tyrosine - metabolism</subject><ispartof>Cancer research (Chicago, Ill.), 1993-05, Vol.53 (9), p.2035-2043</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4709770$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7683250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NOGUCHI, M</creatorcontrib><creatorcontrib>MURAKAMI, M</creatorcontrib><creatorcontrib>BENNETT, W</creatorcontrib><creatorcontrib>LUPU, R</creatorcontrib><creatorcontrib>HUI, F. JR</creatorcontrib><creatorcontrib>HARRIS, C. C</creatorcontrib><creatorcontrib>GERWIN, B. I</creatorcontrib><title>Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>In order to examine the effects of the overexpression of c-erbB-2 (HER-2, neu) on human bronchial epithelial cells, a human c-erbB-2 expression vector was introduced into the simian virus 40 large T-antigen-immortalized human bronchial epithelial cell line BEAS-2B. Isolation of multiple clonal cell lines after selection revealed a wide range of expression of the gene product gp185erbB-2. While three of six clones tested expressed gp185erbB-2 at levels detectable by immunocytochemistry, only one, B2BE6, induced adenocarcinoma-like tumors in athymic nude mice. Both a nontumorigenic clone, B2BE2, and a tumorigenic clone, B2BE6, expressed comparable amounts of gp185erbB-2, which became phosphorylated on tyrosine in response to treatment with the c-erbB-2 ligands gp30 and p75. These data suggest that overexpression of c-erbB-2 in human bronchial epithelial cells can contribute to, but is not sufficient for, induction of tumorigenicity in this human model system.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Bronchi - metabolism</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Epidermal Growth Factor - genetics</subject><subject>Epithelium - metabolism</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Ki-67 Antigen</subject><subject>Ligands</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Neoplasms, Experimental - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Phosphorylation</subject><subject>Phosphotyrosine</subject><subject>Pneumology</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Receptor, ErbB-2</subject><subject>RNA, Messenger - genetics</subject><subject>Transfection</subject><subject>Transforming Growth Factor alpha - genetics</subject><subject>Tumors of the respiratory system and mediastinum</subject><subject>Tyrosine - analogs & derivatives</subject><subject>Tyrosine - metabolism</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLxDAUhYMo4zj6E4QsxF0hzaPJLJ3BFwy40XVJ0ttppE1q0or6622xuLqPczjc-52gdS6YyiTn4hStCSEqE1zSc3SR0vs0ipyIFVrJQjEqyBp971xow9FZ3WIbfIKPEbyFhEONwydE-OojpOSCnzcaD1H7VIMdoMI2g2h2GcVH8ICdx67rQhx0634mtRk77bGJwdvGTenQu6GBdm4ttG26RGe1bhNcLXWD3h7uX_dP2eHl8Xl_d8gaWqghKyqaU2VqSpQsCkm51VTAlquKg9nWpq4IcKaFkpZoaYCYSjHGcikFlZpytkG3f7l9DNNzaSg7l-YLtIcwplIKOVFhs_F6MY6mg6rso-t0_C4XVpN-s-g6TbjqiYR16d_GJdlKSdgvVWV0mg</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>NOGUCHI, M</creator><creator>MURAKAMI, M</creator><creator>BENNETT, W</creator><creator>LUPU, R</creator><creator>HUI, F. 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I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-6d2128bf208766724ca25e948d4eb9fbfd0e43a587c0a7be0bd8333177527a243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Bronchi - metabolism</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Epidermal Growth Factor - genetics</topic><topic>Epithelium - metabolism</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Ki-67 Antigen</topic><topic>Ligands</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Neoplasms, Experimental - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Phosphorylation</topic><topic>Phosphotyrosine</topic><topic>Pneumology</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Receptor, ErbB-2</topic><topic>RNA, Messenger - genetics</topic><topic>Transfection</topic><topic>Transforming Growth Factor alpha - genetics</topic><topic>Tumors of the respiratory system and mediastinum</topic><topic>Tyrosine - analogs & derivatives</topic><topic>Tyrosine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NOGUCHI, M</creatorcontrib><creatorcontrib>MURAKAMI, M</creatorcontrib><creatorcontrib>BENNETT, W</creatorcontrib><creatorcontrib>LUPU, R</creatorcontrib><creatorcontrib>HUI, F. 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I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1993-05-01</date><risdate>1993</risdate><volume>53</volume><issue>9</issue><spage>2035</spage><epage>2043</epage><pages>2035-2043</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>In order to examine the effects of the overexpression of c-erbB-2 (HER-2, neu) on human bronchial epithelial cells, a human c-erbB-2 expression vector was introduced into the simian virus 40 large T-antigen-immortalized human bronchial epithelial cell line BEAS-2B. Isolation of multiple clonal cell lines after selection revealed a wide range of expression of the gene product gp185erbB-2. While three of six clones tested expressed gp185erbB-2 at levels detectable by immunocytochemistry, only one, B2BE6, induced adenocarcinoma-like tumors in athymic nude mice. Both a nontumorigenic clone, B2BE2, and a tumorigenic clone, B2BE6, expressed comparable amounts of gp185erbB-2, which became phosphorylated on tyrosine in response to treatment with the c-erbB-2 ligands gp30 and p75. These data suggest that overexpression of c-erbB-2 in human bronchial epithelial cells can contribute to, but is not sufficient for, induction of tumorigenicity in this human model system.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>7683250</pmid><tpages>9</tpages></addata></record> |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Blotting, Western Bronchi - metabolism Cell Division Cell Line Epidermal Growth Factor - genetics Epithelium - metabolism Gene Expression Humans In Vitro Techniques Ki-67 Antigen Ligands Medical sciences Mice Mice, Nude Neoplasm Proteins - metabolism Neoplasms, Experimental - genetics Nuclear Proteins - metabolism Phosphorylation Phosphotyrosine Pneumology Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Receptor, ErbB-2 RNA, Messenger - genetics Transfection Transforming Growth Factor alpha - genetics Tumors of the respiratory system and mediastinum Tyrosine - analogs & derivatives Tyrosine - metabolism |
| title | Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells |
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