Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development
The cellular and subcellular localization of two GABA transporters, GAT-1 and GAT-3, was investigated using immunocytochemical methods in the rat cerebral cortex and thalamus during postnatal development. The distribution of the transporters is compared with that of the neuronal marker GABA, and wit...
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| Veröffentlicht in: | Cell and tissue research 2003-09, Vol.313 (3), p.245-257 |
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| description | The cellular and subcellular localization of two GABA transporters, GAT-1 and GAT-3, was investigated using immunocytochemical methods in the rat cerebral cortex and thalamus during postnatal development. The distribution of the transporters is compared with that of the neuronal marker GABA, and with that of vimentin and of glial fibrillary acidic protein, which identify immature and mature astrocytes, respectively. Our observations show that the two transporters are already expressed at birth in both brain areas with the same cellular localization as in adult rats, as GAT-1 is present in growth cones and terminals only in the cortex, whereas both transporters are expressed in astrocytes in the cortex and thalamus. The distribution of GAT-1 and GAT-3 undergoes postnatal changes reflecting in general the neurogenetic events of the neocortex and thalamus and, more specifically, the maturation of GABAergic innervation. The adult-like pattern of expression is achieved in the third postnatal week in the cortex and in the second postnatal week in the thalamus. The early expression of GAT-1 in GABAergic terminals confirms previous studies showing the existence of neuronal mechanisms of GABA uptake from the embryonic stages. As for the glial localization, the precocious existence of two astrocytic GABA transporters suggests that they operate through different functional mechanisms from birth, whereas their exclusively glial expression in the thalamus indicates that the astroglia plays a major role in the transport, recycling and metabolism of thalamic GABA. |
| doi_str_mv | 10.1007/s00441-003-0746-9 |
| format | Article |
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The distribution of the transporters is compared with that of the neuronal marker GABA, and with that of vimentin and of glial fibrillary acidic protein, which identify immature and mature astrocytes, respectively. Our observations show that the two transporters are already expressed at birth in both brain areas with the same cellular localization as in adult rats, as GAT-1 is present in growth cones and terminals only in the cortex, whereas both transporters are expressed in astrocytes in the cortex and thalamus. The distribution of GAT-1 and GAT-3 undergoes postnatal changes reflecting in general the neurogenetic events of the neocortex and thalamus and, more specifically, the maturation of GABAergic innervation. The adult-like pattern of expression is achieved in the third postnatal week in the cortex and in the second postnatal week in the thalamus. The early expression of GAT-1 in GABAergic terminals confirms previous studies showing the existence of neuronal mechanisms of GABA uptake from the embryonic stages. As for the glial localization, the precocious existence of two astrocytic GABA transporters suggests that they operate through different functional mechanisms from birth, whereas their exclusively glial expression in the thalamus indicates that the astroglia plays a major role in the transport, recycling and metabolism of thalamic GABA.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-003-0746-9</identifier><identifier>PMID: 12898208</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Aging ; Animals ; Animals, Newborn ; Antibodies, Monoclonal - metabolism ; Astrocytes - metabolism ; Astrocytes - ultrastructure ; Carrier Proteins - metabolism ; Cerebral Cortex - cytology ; Cerebral Cortex - metabolism ; Cerebral Cortex - ultrastructure ; GABA Plasma Membrane Transport Proteins ; gamma-Aminobutyric Acid - metabolism ; Glial Fibrillary Acidic Protein - metabolism ; Immunohistochemistry ; Membrane Proteins - metabolism ; Membrane Transport Proteins - metabolism ; Microscopy, Confocal ; Microscopy, Immunoelectron ; Organic Anion Transporters ; Rats ; Rats, Wistar ; Thalamus - cytology ; Thalamus - metabolism ; Thalamus - ultrastructure ; Vimentin - metabolism</subject><ispartof>Cell and tissue research, 2003-09, Vol.313 (3), p.245-257</ispartof><rights>Copyright Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-b0e88181dd64ae18f589d64889208104028dc3c690a68d0c7f4c08d0f54854f63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12898208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vitellaro-Zuccarello, L</creatorcontrib><creatorcontrib>Calvaresi, N</creatorcontrib><creatorcontrib>De Biasi, S</creatorcontrib><title>Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>The cellular and subcellular localization of two GABA transporters, GAT-1 and GAT-3, was investigated using immunocytochemical methods in the rat cerebral cortex and thalamus during postnatal development. The distribution of the transporters is compared with that of the neuronal marker GABA, and with that of vimentin and of glial fibrillary acidic protein, which identify immature and mature astrocytes, respectively. Our observations show that the two transporters are already expressed at birth in both brain areas with the same cellular localization as in adult rats, as GAT-1 is present in growth cones and terminals only in the cortex, whereas both transporters are expressed in astrocytes in the cortex and thalamus. The distribution of GAT-1 and GAT-3 undergoes postnatal changes reflecting in general the neurogenetic events of the neocortex and thalamus and, more specifically, the maturation of GABAergic innervation. The adult-like pattern of expression is achieved in the third postnatal week in the cortex and in the second postnatal week in the thalamus. The early expression of GAT-1 in GABAergic terminals confirms previous studies showing the existence of neuronal mechanisms of GABA uptake from the embryonic stages. As for the glial localization, the precocious existence of two astrocytic GABA transporters suggests that they operate through different functional mechanisms from birth, whereas their exclusively glial expression in the thalamus indicates that the astroglia plays a major role in the transport, recycling and metabolism of thalamic GABA.</description><subject>Aging</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Astrocytes - metabolism</subject><subject>Astrocytes - ultrastructure</subject><subject>Carrier Proteins - metabolism</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cerebral Cortex - ultrastructure</subject><subject>GABA Plasma Membrane Transport Proteins</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Immunohistochemistry</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Immunoelectron</subject><subject>Organic Anion Transporters</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Thalamus - cytology</subject><subject>Thalamus - metabolism</subject><subject>Thalamus - ultrastructure</subject><subject>Vimentin - metabolism</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkc1q3DAUhUVpaSY_D9BNEV1kFbVXlizLy-kw-YGBblLoTmhkuePBllxJLpNF3j1yZiCQlQ7iu4d7-RD6QuE7Bah-RADOKQFgBCouSP0BLShnBQFZyY9oAQwKUgnx5wydx7gHoFyI-jM6o4WsZQFygZ7XhzHYGDvvsG_x3fLnEqegXRx9SDbEm_z1SCjWrnlN7AZ3DqedxcYGuw26x2YmD69E2uleD1Ocq2Ym6ISbKXTuLx59TE6nzDf2v-39OFiXLtGnVvfRXp3eC_T7dv24uiebX3cPq-WGGFZDIluwUlJJm0ZwbalsS1nnKGWdb6DAoZCNYUbUoIVswFQtN5BDW3JZ8lawC3R97B2D_zfZmNTQRWP7Xjvrp6iqssotosjgt3fg3k_B5d1UQVlVlrSEDNEjZIKPMdhWjaEbdHhSFNQsRh3FqCxGzWJUnWe-noqn7WCbt4mTCfYCdp-HHQ</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Vitellaro-Zuccarello, L</creator><creator>Calvaresi, N</creator><creator>De Biasi, S</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development</title><author>Vitellaro-Zuccarello, L ; Calvaresi, N ; De Biasi, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-b0e88181dd64ae18f589d64889208104028dc3c690a68d0c7f4c08d0f54854f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>Astrocytes - metabolism</topic><topic>Astrocytes - ultrastructure</topic><topic>Carrier Proteins - metabolism</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - metabolism</topic><topic>Cerebral Cortex - ultrastructure</topic><topic>GABA Plasma Membrane Transport Proteins</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Immunohistochemistry</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Immunoelectron</topic><topic>Organic Anion Transporters</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Thalamus - cytology</topic><topic>Thalamus - metabolism</topic><topic>Thalamus - ultrastructure</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vitellaro-Zuccarello, L</creatorcontrib><creatorcontrib>Calvaresi, N</creatorcontrib><creatorcontrib>De Biasi, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vitellaro-Zuccarello, L</au><au>Calvaresi, N</au><au>De Biasi, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>313</volume><issue>3</issue><spage>245</spage><epage>257</epage><pages>245-257</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>The cellular and subcellular localization of two GABA transporters, GAT-1 and GAT-3, was investigated using immunocytochemical methods in the rat cerebral cortex and thalamus during postnatal development. The distribution of the transporters is compared with that of the neuronal marker GABA, and with that of vimentin and of glial fibrillary acidic protein, which identify immature and mature astrocytes, respectively. Our observations show that the two transporters are already expressed at birth in both brain areas with the same cellular localization as in adult rats, as GAT-1 is present in growth cones and terminals only in the cortex, whereas both transporters are expressed in astrocytes in the cortex and thalamus. The distribution of GAT-1 and GAT-3 undergoes postnatal changes reflecting in general the neurogenetic events of the neocortex and thalamus and, more specifically, the maturation of GABAergic innervation. The adult-like pattern of expression is achieved in the third postnatal week in the cortex and in the second postnatal week in the thalamus. The early expression of GAT-1 in GABAergic terminals confirms previous studies showing the existence of neuronal mechanisms of GABA uptake from the embryonic stages. As for the glial localization, the precocious existence of two astrocytic GABA transporters suggests that they operate through different functional mechanisms from birth, whereas their exclusively glial expression in the thalamus indicates that the astroglia plays a major role in the transport, recycling and metabolism of thalamic GABA.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>12898208</pmid><doi>10.1007/s00441-003-0746-9</doi><tpages>13</tpages></addata></record> |
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| subjects | Aging Animals Animals, Newborn Antibodies, Monoclonal - metabolism Astrocytes - metabolism Astrocytes - ultrastructure Carrier Proteins - metabolism Cerebral Cortex - cytology Cerebral Cortex - metabolism Cerebral Cortex - ultrastructure GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid - metabolism Glial Fibrillary Acidic Protein - metabolism Immunohistochemistry Membrane Proteins - metabolism Membrane Transport Proteins - metabolism Microscopy, Confocal Microscopy, Immunoelectron Organic Anion Transporters Rats Rats, Wistar Thalamus - cytology Thalamus - metabolism Thalamus - ultrastructure Vimentin - metabolism |
| title | Expression of GABA transporters, GAT-1 and GAT-3, in the cerebral cortex and thalamus of the rat during postnatal development |
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