Usefulness of quantitative nucleic acid sequence-based amplification for diagnosis of malaria in an academic hospital setting
The aim of the present study was to evaluate the usefulness of quantitative nucleic acid sequence-based amplification (QT-NASBA) to detect Plasmodium spp. in diagnostic specimens of patients suspected of having malaria in a clinical setting in a non-endemic country. During the 4-month recruitment pe...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2003-09, Vol.22 (9), p.555-557 |
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| container_title | European journal of clinical microbiology & infectious diseases |
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| creator | SCHALLIG, H. D. F. H SCHOONE, G. J LOMMERSE, E. J. M KROON, C. C. M DE VRIES, P. J VAN GOOL, T |
| description | The aim of the present study was to evaluate the usefulness of quantitative nucleic acid sequence-based amplification (QT-NASBA) to detect Plasmodium spp. in diagnostic specimens of patients suspected of having malaria in a clinical setting in a non-endemic country. During the 4-month recruitment period, 113 patients were enrolled in the study, of which 93 were diagnosed as non-malaria and 20 as malaria cases on the basis of clinical and microscopic criteria. All microscopically positive cases had QT-NASBA counts of >0.1 parasites/ micro l and there was a significant positive correlation between the parasite counts obtained with both diagnostic methods. Of the 93 microscopically negative cases, six had a positive QT-NASBA result. Three of these cases had a recent history of malaria for which specific treatment was taken. In the other three cases there was no history of malaria and QT-NASBA results in these cases were near the cut-off level (>0.1 parasites/ micro l) of the test. The results demonstrate that QT-NASBA is a useful technology for the diagnosis of malaria in a reference laboratory, and it is very helpful in cases of low parasitemia. |
| doi_str_mv | 10.1007/s10096-003-0985-4 |
| format | Article |
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D. F. H ; SCHOONE, G. J ; LOMMERSE, E. J. M ; KROON, C. C. M ; DE VRIES, P. J ; VAN GOOL, T</creator><creatorcontrib>SCHALLIG, H. D. F. H ; SCHOONE, G. J ; LOMMERSE, E. J. M ; KROON, C. C. M ; DE VRIES, P. J ; VAN GOOL, T</creatorcontrib><description>The aim of the present study was to evaluate the usefulness of quantitative nucleic acid sequence-based amplification (QT-NASBA) to detect Plasmodium spp. in diagnostic specimens of patients suspected of having malaria in a clinical setting in a non-endemic country. During the 4-month recruitment period, 113 patients were enrolled in the study, of which 93 were diagnosed as non-malaria and 20 as malaria cases on the basis of clinical and microscopic criteria. All microscopically positive cases had QT-NASBA counts of >0.1 parasites/ micro l and there was a significant positive correlation between the parasite counts obtained with both diagnostic methods. Of the 93 microscopically negative cases, six had a positive QT-NASBA result. Three of these cases had a recent history of malaria for which specific treatment was taken. In the other three cases there was no history of malaria and QT-NASBA results in these cases were near the cut-off level (>0.1 parasites/ micro l) of the test. The results demonstrate that QT-NASBA is a useful technology for the diagnosis of malaria in a reference laboratory, and it is very helpful in cases of low parasitemia.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-003-0985-4</identifier><identifier>PMID: 12938007</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Academic Medical Centers ; Ambulatory Care ; Animals ; Biological and medical sciences ; Cohort Studies ; Female ; Human protozoal diseases ; Humans ; Infectious diseases ; Malaria ; Malaria, Falciparum - diagnosis ; Male ; Medical sciences ; Netherlands ; Nucleic Acid Amplification Techniques ; Nucleic acids ; Parasites ; Parasitic diseases ; Plasmodium falciparum - isolation & purification ; Protozoal diseases ; RNA, Protozoan - analysis ; Self-Sustained Sequence Replication - methods ; Sensitivity and Specificity ; Vector-borne diseases</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2003-09, Vol.22 (9), p.555-557</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-2b3ac225698537ffa229e55d96b42d0fd739b33fc512555b18a69d88c99ed2a13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15158729$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12938007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCHALLIG, H. D. F. H</creatorcontrib><creatorcontrib>SCHOONE, G. J</creatorcontrib><creatorcontrib>LOMMERSE, E. J. M</creatorcontrib><creatorcontrib>KROON, C. C. M</creatorcontrib><creatorcontrib>DE VRIES, P. J</creatorcontrib><creatorcontrib>VAN GOOL, T</creatorcontrib><title>Usefulness of quantitative nucleic acid sequence-based amplification for diagnosis of malaria in an academic hospital setting</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>The aim of the present study was to evaluate the usefulness of quantitative nucleic acid sequence-based amplification (QT-NASBA) to detect Plasmodium spp. in diagnostic specimens of patients suspected of having malaria in a clinical setting in a non-endemic country. During the 4-month recruitment period, 113 patients were enrolled in the study, of which 93 were diagnosed as non-malaria and 20 as malaria cases on the basis of clinical and microscopic criteria. All microscopically positive cases had QT-NASBA counts of >0.1 parasites/ micro l and there was a significant positive correlation between the parasite counts obtained with both diagnostic methods. Of the 93 microscopically negative cases, six had a positive QT-NASBA result. Three of these cases had a recent history of malaria for which specific treatment was taken. In the other three cases there was no history of malaria and QT-NASBA results in these cases were near the cut-off level (>0.1 parasites/ micro l) of the test. The results demonstrate that QT-NASBA is a useful technology for the diagnosis of malaria in a reference laboratory, and it is very helpful in cases of low parasitemia.</description><subject>Academic Medical Centers</subject><subject>Ambulatory Care</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cohort Studies</subject><subject>Female</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Malaria</subject><subject>Malaria, Falciparum - diagnosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Netherlands</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Nucleic acids</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Plasmodium falciparum - isolation & purification</subject><subject>Protozoal diseases</subject><subject>RNA, Protozoan - analysis</subject><subject>Self-Sustained Sequence Replication - methods</subject><subject>Sensitivity and Specificity</subject><subject>Vector-borne diseases</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkctq3TAURUVpaG6SfkAnRRSamRM9LMsaltAXBDppxuJYj1RBlm4su9BB_r0nvRcCBaEHrL3QYRPyjrMrzpi-briboWNMdsyMqutfkR3vJV6klq_JjhnZd0YLeUrOWntgmBm1fkNOuTByxNeOPN21ELdcQmu0Rvq4QVnTCmv6HWjZXA7JUXDJ0xYet1Bc6CZowVOY9znF5JCshca6UJ_gvtSW_nlmyLAkoKlQwOXAhxlNv2rboz2jbV1Tub8gJxFyC2-P5zm5-_L558237vbH1-83n247J1W_dmKS4IRQAw4pdYwghAlKeTNMvfAsei3NJGV0igul1MRHGIwfR2dM8AK4PCeXB-9-qThGW-2cmgs5Qwl1a1YrzeQgFIIf_gMf6rYU_JsdmeKyF1wgxA-QW2prS4h2v6QZlj-WM_tcjD0UY7EY-1yM7THz_ijepjn4l8SxCQQ-HgFoDnJcoLjUXjjF1agR_gtmBZcg</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>SCHALLIG, H. 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D. F. H</au><au>SCHOONE, G. J</au><au>LOMMERSE, E. J. M</au><au>KROON, C. C. M</au><au>DE VRIES, P. J</au><au>VAN GOOL, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Usefulness of quantitative nucleic acid sequence-based amplification for diagnosis of malaria in an academic hospital setting</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>22</volume><issue>9</issue><spage>555</spage><epage>557</epage><pages>555-557</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><abstract>The aim of the present study was to evaluate the usefulness of quantitative nucleic acid sequence-based amplification (QT-NASBA) to detect Plasmodium spp. in diagnostic specimens of patients suspected of having malaria in a clinical setting in a non-endemic country. During the 4-month recruitment period, 113 patients were enrolled in the study, of which 93 were diagnosed as non-malaria and 20 as malaria cases on the basis of clinical and microscopic criteria. All microscopically positive cases had QT-NASBA counts of >0.1 parasites/ micro l and there was a significant positive correlation between the parasite counts obtained with both diagnostic methods. Of the 93 microscopically negative cases, six had a positive QT-NASBA result. Three of these cases had a recent history of malaria for which specific treatment was taken. In the other three cases there was no history of malaria and QT-NASBA results in these cases were near the cut-off level (>0.1 parasites/ micro l) of the test. 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| subjects | Academic Medical Centers Ambulatory Care Animals Biological and medical sciences Cohort Studies Female Human protozoal diseases Humans Infectious diseases Malaria Malaria, Falciparum - diagnosis Male Medical sciences Netherlands Nucleic Acid Amplification Techniques Nucleic acids Parasites Parasitic diseases Plasmodium falciparum - isolation & purification Protozoal diseases RNA, Protozoan - analysis Self-Sustained Sequence Replication - methods Sensitivity and Specificity Vector-borne diseases |
| title | Usefulness of quantitative nucleic acid sequence-based amplification for diagnosis of malaria in an academic hospital setting |
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