Antisense DNA oligonucleotides II: The use of matrix-assisted laser desorption/ionization mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides
Matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry has been used to measure accurately the molecular masses of synthetic methylphosphonate oligodeoxyribonucleotides, up to 18 nucleotides in length. A simple method has been developed for the complete sequence verification of these...
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| Veröffentlicht in: | Rapid communications in mass spectrometry 1993-03, Vol.7 (3), p.195-200 |
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| container_title | Rapid communications in mass spectrometry |
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| creator | Keough, Thomas Baker, Timothy R. Dobson, Roy L. M. Llacey, Martin P. Riley, Timothy A. Hasselfield, Jody A. Hesselberth, P. Eric |
| description | Matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry has been used to measure accurately the molecular masses of synthetic methylphosphonate oligodeoxyribonucleotides, up to 18 nucleotides in length. A simple method has been developed for the complete sequence verification of these compounds, which are intractable by classical means. Sequencing from the 5′ end of the molecule is possible because of inefficiencies in the synthetic procedure. Complementary information from the 3′ end can be obtained by partial hydrolysis of the methylphosphonate backbone. |
| doi_str_mv | 10.1002/rcm.1290070306 |
| format | Article |
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Sequencing from the 5′ end of the molecule is possible because of inefficiencies in the synthetic procedure. Complementary information from the 3′ end can be obtained by partial hydrolysis of the methylphosphonate backbone.</description><subject>Base Sequence</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Hydrolysis</subject><subject>Lasers</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotides, Antisense - chemical synthesis</subject><subject>Oligonucleotides, Antisense - chemistry</subject><subject>Organic chemistry</subject><subject>Organophosphonates - chemical synthesis</subject><subject>Organophosphonates - chemistry</subject><subject>Reactivity and mechanisms</subject><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGP0zAQhSMEWsrClRuSD4hbunac2Am3UmCptCwCFa3ExXKdMTUkcfG4uy2_jJ-Hq1QFThxGtjTfe37yy7KnjE4ZpcVFMP2UFQ2lknIq7mUTRhuZ04Kz-9mENhXLS9bUD7NHiN8oZawq6Fl2Vpc1q0o6yX7NhugQBgTy-npGfOe--mFrOvDRtYBksXhJlmsg2wR4S3odg9vlGtFhhJZ0GiGQBPqwic4PF2ncT324JhaR4AZMDL6HGPbE-kBiMkP4sYXBALmF4KwzI3-wh7jed5u1xzSDjjAGasHv9sGt_k72OHtgdYfw5HieZ5_fvlnO3-VXHy4X89lVbnhdi7zUjS2bAlprJOeNsMIyIRvByrZYCSErURhTGy5KJlpd81IaXlUAFlhhV7Ti59mL0XcTfEqNUfUODXSdHsBvUclKps-uRAKnI2iCRwxg1Sa4Xoe9YlQdqlKpKvWnqiR4dnTernpoT_ixm7R_ftxrNLqzQQ_G4QkrRVmUgiesGbE718H-P4-qT_P3_0TIR-2hzd1Jq8N3JSSXlbq5vlQ3y3khP76aqy_8N9pzwb8</recordid><startdate>199303</startdate><enddate>199303</enddate><creator>Keough, Thomas</creator><creator>Baker, Timothy R.</creator><creator>Dobson, Roy L. 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M.</creatorcontrib><creatorcontrib>Llacey, Martin P.</creatorcontrib><creatorcontrib>Riley, Timothy A.</creatorcontrib><creatorcontrib>Hasselfield, Jody A.</creatorcontrib><creatorcontrib>Hesselberth, P. Eric</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keough, Thomas</au><au>Baker, Timothy R.</au><au>Dobson, Roy L. M.</au><au>Llacey, Martin P.</au><au>Riley, Timothy A.</au><au>Hasselfield, Jody A.</au><au>Hesselberth, P. Eric</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antisense DNA oligonucleotides II: The use of matrix-assisted laser desorption/ionization mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><addtitle>Rapid Commun. Mass Spectrom</addtitle><date>1993-03</date><risdate>1993</risdate><volume>7</volume><issue>3</issue><spage>195</spage><epage>200</epage><pages>195-200</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>Matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry has been used to measure accurately the molecular masses of synthetic methylphosphonate oligodeoxyribonucleotides, up to 18 nucleotides in length. A simple method has been developed for the complete sequence verification of these compounds, which are intractable by classical means. Sequencing from the 5′ end of the molecule is possible because of inefficiencies in the synthetic procedure. Complementary information from the 3′ end can be obtained by partial hydrolysis of the methylphosphonate backbone.</abstract><cop>London</cop><pub>Heyden & Son Limited</pub><pmid>8481540</pmid><doi>10.1002/rcm.1290070306</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0951-4198 |
| ispartof | Rapid communications in mass spectrometry, 1993-03, Vol.7 (3), p.195-200 |
| issn | 0951-4198 1097-0231 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Base Sequence Chemistry Exact sciences and technology Hydrolysis Lasers Mass spectrometry Mass Spectrometry - methods Molecular Sequence Data Oligonucleotides, Antisense - chemical synthesis Oligonucleotides, Antisense - chemistry Organic chemistry Organophosphonates - chemical synthesis Organophosphonates - chemistry Reactivity and mechanisms |
| title | Antisense DNA oligonucleotides II: The use of matrix-assisted laser desorption/ionization mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides |
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