Detection of hepatitis A virus in shellfish (Mytilus galloprovincialis) with RT-PCR

A PCR assay for the detection of hepatitis A virus (HAV) in shellfish is described. The procedure involves the concentration of viral particles with the use of polyethylene glycol (PEG), followed by viral RNA extraction and purification with oligo(dT) cellulose. Reverse transcriptase-PCR detection w...

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Veröffentlicht in:	Journal of food protection 2003-09, Vol.66 (9), p.1681-1685
Hauptverfasser:	Pinto, A. di, Forte, V.T, Tantillo, G.M, Terio, V, Buonavoglia, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals assays Biological and medical sciences Bivalvia - microbiology Consumer Product Safety detection Fish and seafood industries food contamination Food industries Food microbiology food pathogens food safety Fundamental and applied biological sciences. Psychology Hepatitis A virus Hepatitis A virus - genetics Hepatitis A virus - isolation & purification mussels Mytilus galloprovincialis polymerase chain reaction real-time polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - isolation & purification Sensitivity and Specificity shellfish Shellfish - microbiology Time Factors
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