Influence of the quantity of nonspecific DNA and repeated freezing and thawing of samples on the quantification of DNA by the Light Cycler

Quantification of DNA in real-time using the Light Cycler® is increasingly being used for the detection and follow-up of various infectious and other diseases. We evaluated the effect of two parameters, namely the presence of nonspecific DNA and prior repeated freezing and thawing on the accurate qu...

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Veröffentlicht in:	Journal of microbiological methods 2003-10, Vol.55 (1), p.213-219
Hauptverfasser:	Bellete, B, Flori, P, Hafid, J, Raberin, H, Tran Manh Sung, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences DNA - analysis DNA - chemistry Freeze–thawing Freezing Fundamental and applied biological sciences. Psychology Light Cycler Microbiology Nonspecific DNA Polymerase Chain Reaction - instrumentation
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