A PCR [polymerase chain reaction] primer-specific to Cylindrocarpon heteronema for detection of the pathogen in apple wood

An oligonucleotide Primer (ChInt) was synthesised from the variable internally transcribed spacer (ITS) 1 region of ribosomal DNA (rDNA) of Cylindrocarpon heteronema. PCR with primers ChInt and ITS4 (from a conserved sequence of the rDNA) amplified a 470-bp fragment from several isolates of C hetero...

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Veröffentlicht in:	FEMS microbiology letters 1993-03, Vol.108 (1), p.117-120
Hauptverfasser:	Brown, A.E. (Queens's Univ., Belfast (United Kingdom). Dept. of Applied Plant Science), Muthumeenakshi, S, Sreenivasaprasad, S, Mills, P.R, Swinburne, T.R
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Agronomy. Soil science and plant productions Base Sequence Biological and medical sciences cankers CYLINDROCARPON Cylindrocarpon heteronema detection DIAGNOSIS DIAGNOSTIC Diagnostic polymerase chain reaction diagnostic techniques DIAGNOSTICO DNA DNA Probes DNA purification DNA, Fungal - genetics DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology MALUS Microbiology Mitosporic Fungi - genetics Mitosporic Fungi - isolation & purification Mitosporic Fungi - pathogenicity Molecular Sequence Data Mycological methods and techniques used in mycology Mycology Nectria galligena nucleotide sequences Oligonucleotide primer Plants - microbiology Polymerase Chain Reaction Polymorphism, Genetic Wood
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