Cell seeding in porous transplantation devices
Porous laminated discs of 1.35 cm diameter and thickness of 0.5 cm fashioned from biodegradable polymers were used as scaffolds for the transplantation of isolated cell populations. The distribution of cells seeded in these devices via injection was modelled with a system of dyed polymeric micropart...
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| Veröffentlicht in: | Biomaterials 1993, Vol.14 (4), p.270-278 |
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| creator | Wald, Heidi L. Sarakinos, Georgios Lyman, Michelle D. Mikos, Antonios G. Vacanti, Joseph P. Langer, Robert |
| description | Porous laminated discs of 1.35 cm diameter and thickness of 0.5 cm fashioned from biodegradable polymers were used as scaffolds for the transplantation of isolated cell populations. The distribution of cells seeded in these devices via injection was modelled with a system of dyed polymeric microparticles. Optimization of parameters related to device design and surgical injection conditions was carried out to maximize the device volume effectively employed in cell transplantation. The area of distribution on the top surface of each device was determined by image analysis techniques and used as a measure of the spatial distribution of injected particles. For poly(
l-lactic acid) devices of porosity of 0.83 and median pore diameter of 166μm seeded with 6,μm beads under standard injection conditions, the average surface area of distribution was 44.45% (±3.36%). The device pore size was found to be a crucial determinant of particle distribution, whilst particle size in the range of 1–10 μm was not found to be important for the devices tested. Application of these results to the seeding of hepatocyte suspensions was made. |
| doi_str_mv | 10.1016/0142-9612(93)90117-K |
| format | Article |
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l-lactic acid) devices of porosity of 0.83 and median pore diameter of 166μm seeded with 6,μm beads under standard injection conditions, the average surface area of distribution was 44.45% (±3.36%). The device pore size was found to be a crucial determinant of particle distribution, whilst particle size in the range of 1–10 μm was not found to be important for the devices tested. Application of these results to the seeding of hepatocyte suspensions was made.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/0142-9612(93)90117-K</identifier><identifier>PMID: 8476996</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biocompatible Materials ; biodegradable polymer ; Biological and medical sciences ; Cell Division - physiology ; cell seeding ; Cell transplantation ; Image Processing, Computer-Assisted ; Lactates - chemistry ; Lactic Acid ; Liver - cytology ; Liver Transplantation - methods ; Medical sciences ; Microscopy, Electron ; Microspheres ; Particle Size ; poly( l-lactic acid) device ; Polyesters ; Polymers - chemistry ; Porosity ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Rats ; Technology. Biomaterials. Equipments. Material. Instrumentation ; Tissue Transplantation - methods</subject><ispartof>Biomaterials, 1993, Vol.14 (4), p.270-278</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-38eeace366c5ed95c86958c9413f13e0f2ff721898a6c78483389f60c2504a0c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0142-9612(93)90117-K$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4649111$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8476996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wald, Heidi L.</creatorcontrib><creatorcontrib>Sarakinos, Georgios</creatorcontrib><creatorcontrib>Lyman, Michelle D.</creatorcontrib><creatorcontrib>Mikos, Antonios G.</creatorcontrib><creatorcontrib>Vacanti, Joseph P.</creatorcontrib><creatorcontrib>Langer, Robert</creatorcontrib><title>Cell seeding in porous transplantation devices</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Porous laminated discs of 1.35 cm diameter and thickness of 0.5 cm fashioned from biodegradable polymers were used as scaffolds for the transplantation of isolated cell populations. The distribution of cells seeded in these devices via injection was modelled with a system of dyed polymeric microparticles. Optimization of parameters related to device design and surgical injection conditions was carried out to maximize the device volume effectively employed in cell transplantation. The area of distribution on the top surface of each device was determined by image analysis techniques and used as a measure of the spatial distribution of injected particles. For poly(
l-lactic acid) devices of porosity of 0.83 and median pore diameter of 166μm seeded with 6,μm beads under standard injection conditions, the average surface area of distribution was 44.45% (±3.36%). The device pore size was found to be a crucial determinant of particle distribution, whilst particle size in the range of 1–10 μm was not found to be important for the devices tested. Application of these results to the seeding of hepatocyte suspensions was made.</description><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>biodegradable polymer</subject><subject>Biological and medical sciences</subject><subject>Cell Division - physiology</subject><subject>cell seeding</subject><subject>Cell transplantation</subject><subject>Image Processing, Computer-Assisted</subject><subject>Lactates - chemistry</subject><subject>Lactic Acid</subject><subject>Liver - cytology</subject><subject>Liver Transplantation - methods</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Microspheres</subject><subject>Particle Size</subject><subject>poly( l-lactic acid) device</subject><subject>Polyesters</subject><subject>Polymers - chemistry</subject><subject>Porosity</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Rats</subject><subject>Technology. Biomaterials. Equipments. Material. Instrumentation</subject><subject>Tissue Transplantation - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcIexI_D1mTzfRGk-EULXvQcYnZWItvdNdkW_Pemdumxp2GYZ2ZeHoTOCZ4STMQdJqzItSDFjaa3GhMi8_kBGhMlVc415odovEOO0UmM3zj1mBUjNFJMCq3FGE1nUNdZBCh985X5Juva0K5i1gfbxK62TW973zZZCWvvIJ6io8rWEc6GOkEfT4_vs5d88fb8OntY5I4R1edUAVgHVAjHodTcKaG5cpoRWhEKuCqqShZEaWWFk4opSpWuBHYFx8xiRyfoanu3C-3PCmJvlj66FNU2kOIZyYUuJJcJvN4PMi64EJQnkm1JF9oYA1SmC35pw68h2GyEmo0ts7FlNDX_Qs08rV0MD1afSyh3S4PBNL8c5jY6W1fJm_NxhzHBNCEkYfdbDJK1tYdgovPQuOQ9gOtN2fr9Of4A7hqPvA</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Wald, Heidi L.</creator><creator>Sarakinos, Georgios</creator><creator>Lyman, Michelle D.</creator><creator>Mikos, Antonios G.</creator><creator>Vacanti, Joseph P.</creator><creator>Langer, Robert</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Cell seeding in porous transplantation devices</title><author>Wald, Heidi L. ; Sarakinos, Georgios ; Lyman, Michelle D. ; Mikos, Antonios G. ; Vacanti, Joseph P. ; Langer, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-38eeace366c5ed95c86958c9413f13e0f2ff721898a6c78483389f60c2504a0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>biodegradable polymer</topic><topic>Biological and medical sciences</topic><topic>Cell Division - physiology</topic><topic>cell seeding</topic><topic>Cell transplantation</topic><topic>Image Processing, Computer-Assisted</topic><topic>Lactates - chemistry</topic><topic>Lactic Acid</topic><topic>Liver - cytology</topic><topic>Liver Transplantation - methods</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Microspheres</topic><topic>Particle Size</topic><topic>poly( l-lactic acid) device</topic><topic>Polyesters</topic><topic>Polymers - chemistry</topic><topic>Porosity</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Rats</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><topic>Tissue Transplantation - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wald, Heidi L.</creatorcontrib><creatorcontrib>Sarakinos, Georgios</creatorcontrib><creatorcontrib>Lyman, Michelle D.</creatorcontrib><creatorcontrib>Mikos, Antonios G.</creatorcontrib><creatorcontrib>Vacanti, Joseph P.</creatorcontrib><creatorcontrib>Langer, Robert</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wald, Heidi L.</au><au>Sarakinos, Georgios</au><au>Lyman, Michelle D.</au><au>Mikos, Antonios G.</au><au>Vacanti, Joseph P.</au><au>Langer, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell seeding in porous transplantation devices</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>1993</date><risdate>1993</risdate><volume>14</volume><issue>4</issue><spage>270</spage><epage>278</epage><pages>270-278</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Porous laminated discs of 1.35 cm diameter and thickness of 0.5 cm fashioned from biodegradable polymers were used as scaffolds for the transplantation of isolated cell populations. The distribution of cells seeded in these devices via injection was modelled with a system of dyed polymeric microparticles. Optimization of parameters related to device design and surgical injection conditions was carried out to maximize the device volume effectively employed in cell transplantation. The area of distribution on the top surface of each device was determined by image analysis techniques and used as a measure of the spatial distribution of injected particles. For poly(
l-lactic acid) devices of porosity of 0.83 and median pore diameter of 166μm seeded with 6,μm beads under standard injection conditions, the average surface area of distribution was 44.45% (±3.36%). The device pore size was found to be a crucial determinant of particle distribution, whilst particle size in the range of 1–10 μm was not found to be important for the devices tested. Application of these results to the seeding of hepatocyte suspensions was made.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8476996</pmid><doi>10.1016/0142-9612(93)90117-K</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biomaterials, 1993, Vol.14 (4), p.270-278 |
| issn | 0142-9612 1878-5905 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biocompatible Materials biodegradable polymer Biological and medical sciences Cell Division - physiology cell seeding Cell transplantation Image Processing, Computer-Assisted Lactates - chemistry Lactic Acid Liver - cytology Liver Transplantation - methods Medical sciences Microscopy, Electron Microspheres Particle Size poly( l-lactic acid) device Polyesters Polymers - chemistry Porosity Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Rats Technology. Biomaterials. Equipments. Material. Instrumentation Tissue Transplantation - methods |
| title | Cell seeding in porous transplantation devices |
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