Chromosomes in Living Escherichia coli Cells are Segregated into Domains of Supercoiling

Torsional tension in the DNA double helix can be detected in living cells of Escherichia coli from measurements of the rate of trimethylpsoralen photobinding to the intracellular DNA. Here we show that this tension is relaxed in vivo when single-strand DNA breaks are introduced by γ -irradiation and...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1981-01, Vol.78 (1), p.224-228
Hauptverfasser:	Sinden, Richard R., Pettijohn, David E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell growth Cell separation Chromosomes Chromosomes, Bacterial - drug effects Chromosomes, Bacterial - radiation effects Chromosomes, Bacterial - ultrastructure DNA DNA, Bacterial - analysis DNA, Bacterial - radiation effects DNA, Single-Stranded - analysis DNA, Superhelical - analysis DNA, Superhelical - radiation effects Dopamine - metabolism Escherichia coli - genetics Escherichia coli - ultrastructure Gamma cells Gamma Rays Genomes Irradiation L cells Light Molecules Nucleic Acid Conformation RNA RNA, Bacterial - biosynthesis Trioxsalen - pharmacology
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