Diethyldithiocarbamate inhibits induction of macrophage NO synthase
We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow-derived macrophages. A short exposure (between 1 and 16 h) of L929-cell medium-preconditioned macrophages...
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| Veröffentlicht in: | FEBS letters 1993-04, Vol.321 (2), p.215-218 |
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| creator | Mülsch, Alexander Schray-Utz, Beate Mordvintcev, Peter I. Hauschildt, Sunna Busse, Rudi |
| description | We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow-derived macrophages. A short exposure (between 1 and 16 h) of L929-cell medium-preconditioned macrophages to
E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1–1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of, the gene encoding inducible NOS. |
| doi_str_mv | 10.1016/0014-5793(93)80111-7 |
| format | Article |
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E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1–1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of, the gene encoding inducible NOS.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(93)80111-7</identifier><identifier>PMID: 7682975</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Oxidoreductases - biosynthesis ; Amino Acid Oxidoreductases - genetics ; Animals ; Biological and medical sciences ; Blotting, Northern ; Bone Marrow - drug effects ; Bone Marrow - enzymology ; Bone Marrow Cells ; Bone marrow-derived macrophage ; Cells, Cultured ; Culture Media, Conditioned ; Diethyldithiocarbamate ; Ditiocarb - pharmacology ; DNA Probes ; Enzyme Induction - drug effects ; Fundamental and applied biological sciences. Psychology ; L Cells (Cell Line) ; Macrophages - cytology ; Macrophages - drug effects ; Macrophages - enzymology ; Mice ; Mice, Inbred BALB C ; Molecular and cellular biology ; Molecular genetics ; NF-kappa B - antagonists & inhibitors ; NFkappaB ; Nitric Oxide Synthase ; Nitrites - metabolism ; NO synthase ; RNA, Messenger - analysis ; RNA, Messenger - metabolism ; Transcription ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>FEBS letters, 1993-04, Vol.321 (2), p.215-218</ispartof><rights>1993</rights><rights>FEBS Letters 321 (1993) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5267-d8effd30755392a41d7bcd46f1c56e3a5c4f6d2d70d3b0237aca5ddb70ca66253</citedby><cites>FETCH-LOGICAL-c5267-d8effd30755392a41d7bcd46f1c56e3a5c4f6d2d70d3b0237aca5ddb70ca66253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579393801117$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4710547$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7682975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mülsch, Alexander</creatorcontrib><creatorcontrib>Schray-Utz, Beate</creatorcontrib><creatorcontrib>Mordvintcev, Peter I.</creatorcontrib><creatorcontrib>Hauschildt, Sunna</creatorcontrib><creatorcontrib>Busse, Rudi</creatorcontrib><title>Diethyldithiocarbamate inhibits induction of macrophage NO synthase</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow-derived macrophages. A short exposure (between 1 and 16 h) of L929-cell medium-preconditioned macrophages to
E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1–1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of, the gene encoding inducible NOS.</description><subject>Amino Acid Oxidoreductases - biosynthesis</subject><subject>Amino Acid Oxidoreductases - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Bone Marrow - drug effects</subject><subject>Bone Marrow - enzymology</subject><subject>Bone Marrow Cells</subject><subject>Bone marrow-derived macrophage</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned</subject><subject>Diethyldithiocarbamate</subject><subject>Ditiocarb - pharmacology</subject><subject>DNA Probes</subject><subject>Enzyme Induction - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>L Cells (Cell Line)</subject><subject>Macrophages - cytology</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - enzymology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>NFkappaB</subject><subject>Nitric Oxide Synthase</subject><subject>Nitrites - metabolism</subject><subject>NO synthase</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtP3DAUhS0EogPtPwApi6qiixQ7fiUbJDrMQCUEm3Zt3dg3xFUeUzvTav49ycxolgXJku17vnvsewi5YPQbo0xdU8pEKnXBrwr-NaeMsVQfkRnLNU-5UPkxmR2QD-Qsxt90vOesOCWnWuVZoeWMzO88DvWmcX6ofW8hlNDCgInval_6IY4Ht7aD77ukr5IWbOhXNbxg8vScxE031BDxIzmpoIn4ab-fk1_Lxc_5Q_r4fP9jfvuYWpkpnbocq8pxqqXkRQaCOV1aJ1TFrFTIQVpRKZc5TR0vacY1WJDOlZpaUCqT_Jx82fmuQv9njXEwrY8WmwY67NfRaKkKqoV-E2QTx6kYQbEDx7FiDFiZVfAthI1h1EwhmylBMyVoxrUN2Uz-l3v_ddmiOzTtUx31z3sdooWmCtBZHw-Y0IzK7TeXO-yfb3DzrqfNcvE9m4SpXvBtdTK62RnhmP5fj8FE67Gz6HxAOxjX-_8P9Arg06t3</recordid><startdate>19930426</startdate><enddate>19930426</enddate><creator>Mülsch, Alexander</creator><creator>Schray-Utz, Beate</creator><creator>Mordvintcev, Peter I.</creator><creator>Hauschildt, Sunna</creator><creator>Busse, Rudi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19930426</creationdate><title>Diethyldithiocarbamate inhibits induction of macrophage NO synthase</title><author>Mülsch, Alexander ; Schray-Utz, Beate ; Mordvintcev, Peter I. ; Hauschildt, Sunna ; Busse, Rudi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5267-d8effd30755392a41d7bcd46f1c56e3a5c4f6d2d70d3b0237aca5ddb70ca66253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Oxidoreductases - biosynthesis</topic><topic>Amino Acid Oxidoreductases - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Bone Marrow - drug effects</topic><topic>Bone Marrow - enzymology</topic><topic>Bone Marrow Cells</topic><topic>Bone marrow-derived macrophage</topic><topic>Cells, Cultured</topic><topic>Culture Media, Conditioned</topic><topic>Diethyldithiocarbamate</topic><topic>Ditiocarb - pharmacology</topic><topic>DNA Probes</topic><topic>Enzyme Induction - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>L Cells (Cell Line)</topic><topic>Macrophages - cytology</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - enzymology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>NFkappaB</topic><topic>Nitric Oxide Synthase</topic><topic>Nitrites - metabolism</topic><topic>NO synthase</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcription</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mülsch, Alexander</creatorcontrib><creatorcontrib>Schray-Utz, Beate</creatorcontrib><creatorcontrib>Mordvintcev, Peter I.</creatorcontrib><creatorcontrib>Hauschildt, Sunna</creatorcontrib><creatorcontrib>Busse, Rudi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mülsch, Alexander</au><au>Schray-Utz, Beate</au><au>Mordvintcev, Peter I.</au><au>Hauschildt, Sunna</au><au>Busse, Rudi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diethyldithiocarbamate inhibits induction of macrophage NO synthase</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1993-04-26</date><risdate>1993</risdate><volume>321</volume><issue>2</issue><spage>215</spage><epage>218</epage><pages>215-218</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow-derived macrophages. A short exposure (between 1 and 16 h) of L929-cell medium-preconditioned macrophages to
E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1–1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of, the gene encoding inducible NOS.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7682975</pmid><doi>10.1016/0014-5793(93)80111-7</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Oxidoreductases - biosynthesis Amino Acid Oxidoreductases - genetics Animals Biological and medical sciences Blotting, Northern Bone Marrow - drug effects Bone Marrow - enzymology Bone Marrow Cells Bone marrow-derived macrophage Cells, Cultured Culture Media, Conditioned Diethyldithiocarbamate Ditiocarb - pharmacology DNA Probes Enzyme Induction - drug effects Fundamental and applied biological sciences. Psychology L Cells (Cell Line) Macrophages - cytology Macrophages - drug effects Macrophages - enzymology Mice Mice, Inbred BALB C Molecular and cellular biology Molecular genetics NF-kappa B - antagonists & inhibitors NFkappaB Nitric Oxide Synthase Nitrites - metabolism NO synthase RNA, Messenger - analysis RNA, Messenger - metabolism Transcription Transcription. Transcription factor. Splicing. Rna processing |
| title | Diethyldithiocarbamate inhibits induction of macrophage NO synthase |
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