Kinetic studies on the peroxidase activity of selenosubtilisin

Selenosubtilisin, a semisynthetic selenoenzyme produced by chemical modification of the serine protease subtilisin, acts as a mimic of glutathione peroxidase, catalyzing the reduction of tert-butyl hydroperoxide by 3-carboxy-4-nitrobenzenethiol. To clarify the mechanism of action of this catalyst, d...

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Veröffentlicht in:	Biochemistry (Easton) 1993-04, Vol.32 (14), p.3754-3762
Hauptverfasser:	Bell, Ian M, Fisher, Megan L, Wu, Zhen Ping, Hilvert, Donald
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Binding Sites Biological and medical sciences Chromatography, Affinity Chromatography, Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Glutathione Peroxidase - metabolism Hydrogen-Ion Concentration Hydrolases Iodoacetamide - pharmacology Kinetics Magnetic Resonance Spectroscopy Mass Spectrometry Oxidation-Reduction Peroxidase - metabolism Peroxides - metabolism Spectrophotometry, Ultraviolet Substrate Specificity Subtilisins - antagonists & inhibitors Subtilisins - metabolism Sulfhydryl Compounds - metabolism tert-Butylhydroperoxide X-Ray Diffraction
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