Serum antibodies (measured by M RSPAH) to alcohol-soluble gliadins in adult coeliac patients
Gliadin antibodies of different immunoglobulin classes may be measured very satisfactorily by M RSPAH (mixed reverse (solid phase) passive antiglobulin haemadsorption) using an alcoholic solution of gliadin proteins in a microtitre plate test. We report the antibody levels in 20 healthy control pers...
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Veröffentlicht in: | Journal of immunological methods 1981, Vol.42 (2), p.129-136 |
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container_issue | 2 |
container_start_page | 129 |
container_title | Journal of immunological methods |
container_volume | 42 |
creator | Kieffer, Marianne Frazier, P.J. Daniels, N.W.R. Ciclitira, P.J. Coombs, R.R.A. |
description | Gliadin antibodies of different immunoglobulin classes may be measured very satisfactorily by M
RSPAH (mixed reverse (solid phase) passive antiglobulin haemadsorption) using an alcoholic solution of gliadin proteins in a microtitre plate test.
We report the antibody levels in 20 healthy control persons, 12 recently diagnosed coeliac cases, 21 patients on a prescribed gluten-free diet and 22 patients with inflammatory bowel disease. |
doi_str_mv | 10.1016/0022-1759(81)90142-3 |
format | Article |
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RSPAH (mixed reverse (solid phase) passive antiglobulin haemadsorption) using an alcoholic solution of gliadin proteins in a microtitre plate test.
We report the antibody levels in 20 healthy control persons, 12 recently diagnosed coeliac cases, 21 patients on a prescribed gluten-free diet and 22 patients with inflammatory bowel disease.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(81)90142-3</identifier><identifier>PMID: 7240756</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antibodies - analysis ; Celiac Disease - immunology ; Colitis, Ulcerative - immunology ; Crohn Disease - immunology ; Ethanol ; Gliadin - immunology ; Hemagglutination Tests - methods ; Humans ; Immunoglobulin A - analysis ; Immunoglobulin G - analysis ; Immunoglobulin M - analysis ; Plant Proteins - immunology ; Solubility</subject><ispartof>Journal of immunological methods, 1981, Vol.42 (2), p.129-136</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-d63afb3156dbc3350d6b642f0d0b22c7b8b50fdbae240f3e725196a87e6cb6ab3</citedby><cites>FETCH-LOGICAL-c272t-d63afb3156dbc3350d6b642f0d0b22c7b8b50fdbae240f3e725196a87e6cb6ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(81)90142-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7240756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kieffer, Marianne</creatorcontrib><creatorcontrib>Frazier, P.J.</creatorcontrib><creatorcontrib>Daniels, N.W.R.</creatorcontrib><creatorcontrib>Ciclitira, P.J.</creatorcontrib><creatorcontrib>Coombs, R.R.A.</creatorcontrib><title>Serum antibodies (measured by M RSPAH) to alcohol-soluble gliadins in adult coeliac patients</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Gliadin antibodies of different immunoglobulin classes may be measured very satisfactorily by M
RSPAH (mixed reverse (solid phase) passive antiglobulin haemadsorption) using an alcoholic solution of gliadin proteins in a microtitre plate test.
We report the antibody levels in 20 healthy control persons, 12 recently diagnosed coeliac cases, 21 patients on a prescribed gluten-free diet and 22 patients with inflammatory bowel disease.</description><subject>Antibodies - analysis</subject><subject>Celiac Disease - immunology</subject><subject>Colitis, Ulcerative - immunology</subject><subject>Crohn Disease - immunology</subject><subject>Ethanol</subject><subject>Gliadin - immunology</subject><subject>Hemagglutination Tests - methods</subject><subject>Humans</subject><subject>Immunoglobulin A - analysis</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin M - analysis</subject><subject>Plant Proteins - immunology</subject><subject>Solubility</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kElrHDEQhUVIcMbLP0hApzBzaKckTUs9l4AxXgIODl5uBqGlOlFQt8ZSt2H-vXsyg48-FdR79arqI-QLg1MGTH4H4Lxiql7NG7ZYAVvySnwgM9YoXqkV1B_J7M3ymRyW8g8AGEg4IAeKL0HVckae7jGPHTX9EGzyAQudd2jKmNFTu6G_6N3977PrBR0SNdGlvylWJcXRRqR_YjA-9IWGnho_xoG6hFPP0bUZAvZDOSafWhMLnuzrEXm8vHg4v65ubq9-np_dVI4rPlReCtNawWrprROiBi-tXPIWPFjOnbKNraH11uB0dStQ8ZqtpGkUSmelseKIfNvlrnN6HrEMugvFYYymxzQWPX3aiIY1k3G5M7qcSsnY6nUOnckbzUBvoeotMb0lphum_0PVYhr7us8fbYf-bWhPcdJ_7HScnnwJmHVxEwCHPmR0g_YpvL_gFW3Vhf4</recordid><startdate>1981</startdate><enddate>1981</enddate><creator>Kieffer, Marianne</creator><creator>Frazier, P.J.</creator><creator>Daniels, N.W.R.</creator><creator>Ciclitira, P.J.</creator><creator>Coombs, R.R.A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1981</creationdate><title>Serum antibodies (measured by M RSPAH) to alcohol-soluble gliadins in adult coeliac patients</title><author>Kieffer, Marianne ; Frazier, P.J. ; Daniels, N.W.R. ; Ciclitira, P.J. ; Coombs, R.R.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-d63afb3156dbc3350d6b642f0d0b22c7b8b50fdbae240f3e725196a87e6cb6ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Antibodies - analysis</topic><topic>Celiac Disease - immunology</topic><topic>Colitis, Ulcerative - immunology</topic><topic>Crohn Disease - immunology</topic><topic>Ethanol</topic><topic>Gliadin - immunology</topic><topic>Hemagglutination Tests - methods</topic><topic>Humans</topic><topic>Immunoglobulin A - analysis</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin M - analysis</topic><topic>Plant Proteins - immunology</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kieffer, Marianne</creatorcontrib><creatorcontrib>Frazier, P.J.</creatorcontrib><creatorcontrib>Daniels, N.W.R.</creatorcontrib><creatorcontrib>Ciclitira, P.J.</creatorcontrib><creatorcontrib>Coombs, R.R.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kieffer, Marianne</au><au>Frazier, P.J.</au><au>Daniels, N.W.R.</au><au>Ciclitira, P.J.</au><au>Coombs, R.R.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum antibodies (measured by M RSPAH) to alcohol-soluble gliadins in adult coeliac patients</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1981</date><risdate>1981</risdate><volume>42</volume><issue>2</issue><spage>129</spage><epage>136</epage><pages>129-136</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>Gliadin antibodies of different immunoglobulin classes may be measured very satisfactorily by M
RSPAH (mixed reverse (solid phase) passive antiglobulin haemadsorption) using an alcoholic solution of gliadin proteins in a microtitre plate test.
We report the antibody levels in 20 healthy control persons, 12 recently diagnosed coeliac cases, 21 patients on a prescribed gluten-free diet and 22 patients with inflammatory bowel disease.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7240756</pmid><doi>10.1016/0022-1759(81)90142-3</doi><tpages>8</tpages></addata></record> |
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subjects | Antibodies - analysis Celiac Disease - immunology Colitis, Ulcerative - immunology Crohn Disease - immunology Ethanol Gliadin - immunology Hemagglutination Tests - methods Humans Immunoglobulin A - analysis Immunoglobulin G - analysis Immunoglobulin M - analysis Plant Proteins - immunology Solubility |
title | Serum antibodies (measured by M RSPAH) to alcohol-soluble gliadins in adult coeliac patients |
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