Transforming growth factor-beta 2 prevents preterm delivery induced by interleukin-1 alpha and tumor necrosis factor-alpha in the rabbit
The purpose of the study was to determine whether preterm parturition in the rabbit can be induced by intraamniotic injection of proinflammatory cytokines, interleukin-1 alpha and tumor necrosis factor-alpha, and whether transforming growth factor-beta 2, an inhibitor of the cytokine-induced prostag...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1993-04, Vol.168 (4), p.1318-1322 |
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| creator | Bry, K Hallman, M |
| description | The purpose of the study was to determine whether preterm parturition in the rabbit can be induced by intraamniotic injection of proinflammatory cytokines, interleukin-1 alpha and tumor necrosis factor-alpha, and whether transforming growth factor-beta 2, an inhibitor of the cytokine-induced prostaglandin synthesis, modifies the effect of these cytokines.
New Zealand White rabbits were injected in each amniotic cavity on day 24 of gestation with one of the following: a combination of interleukin-1 alpha (150 ng) and tumor necrosis factor-alpha (1.25 micrograms), 50 ng of transforming growth factor-beta 2 concomitantly with interleukin-1 alpha and tumor necrosis factor-alpha, or vehicle. In the first study the animals were observed for signs of delivery until day 29 of gestation. In the second study the effect of transforming growth factor-beta 2 (50 ng/fetus) on the rate of premature delivery was evaluated. In the third study the concentrations of prostaglandin E2 and 13,14-dihydro-15-keto-prostaglandin F2 alpha were measured in the amniotic fluid on day 27 of gestation. The statistics used were Fisher's exact test, the chi 2 test, and the Mann-Whitney U test.
Altogether 61 of 191 fetuses (32%) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha group, whereas only two of 161 fetuses (1.2%) (p = 0.0001) and one of 159 (0.6%) (p = 0.0001) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group and in the control group, respectively. Of the 23 animals injected with interleukin-1 alpha and tumor necrosis factor-alpha, six (26%) delivered all of their fetuses prematurely versus none in the other groups (p = 0.02). None of the 88 fetuses in the transforming growth factor-beta 2 group were born prematurely. The prostaglandin E2 concentrations in the amniotic fluids were higher in the interleukin-1 alpha-tumor necrosis factor-alpha group than in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group (p = 0.05) or in the control group (p = 0.02).
Preterm parturition can be provoked in the rabbit by intraamniotic injections of interleukin-1 alpha and tumor necrosis factor-alpha. Transforming growth factor-beta 2 prevents the cytokine-induced increase in premature delivery. |
| doi_str_mv | 10.1016/0002-9378(93)90388-Y |
| format | Article |
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New Zealand White rabbits were injected in each amniotic cavity on day 24 of gestation with one of the following: a combination of interleukin-1 alpha (150 ng) and tumor necrosis factor-alpha (1.25 micrograms), 50 ng of transforming growth factor-beta 2 concomitantly with interleukin-1 alpha and tumor necrosis factor-alpha, or vehicle. In the first study the animals were observed for signs of delivery until day 29 of gestation. In the second study the effect of transforming growth factor-beta 2 (50 ng/fetus) on the rate of premature delivery was evaluated. In the third study the concentrations of prostaglandin E2 and 13,14-dihydro-15-keto-prostaglandin F2 alpha were measured in the amniotic fluid on day 27 of gestation. The statistics used were Fisher's exact test, the chi 2 test, and the Mann-Whitney U test.
Altogether 61 of 191 fetuses (32%) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha group, whereas only two of 161 fetuses (1.2%) (p = 0.0001) and one of 159 (0.6%) (p = 0.0001) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group and in the control group, respectively. Of the 23 animals injected with interleukin-1 alpha and tumor necrosis factor-alpha, six (26%) delivered all of their fetuses prematurely versus none in the other groups (p = 0.02). None of the 88 fetuses in the transforming growth factor-beta 2 group were born prematurely. The prostaglandin E2 concentrations in the amniotic fluids were higher in the interleukin-1 alpha-tumor necrosis factor-alpha group than in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group (p = 0.05) or in the control group (p = 0.02).
Preterm parturition can be provoked in the rabbit by intraamniotic injections of interleukin-1 alpha and tumor necrosis factor-alpha. Transforming growth factor-beta 2 prevents the cytokine-induced increase in premature delivery.</description><identifier>ISSN: 0002-9378</identifier><identifier>DOI: 10.1016/0002-9378(93)90388-Y</identifier><identifier>PMID: 8475982</identifier><language>eng</language><publisher>United States</publisher><subject>Amniotic Fluid - chemistry ; Animals ; Dinoprost - analogs & derivatives ; Dinoprost - analysis ; Dinoprostone - analysis ; Female ; Interleukin-1 - antagonists & inhibitors ; Interleukin-1 - pharmacology ; Obstetric Labor, Premature - chemically induced ; Obstetric Labor, Premature - metabolism ; Obstetric Labor, Premature - prevention & control ; Pregnancy ; Progesterone - blood ; Rabbits ; Transforming Growth Factor beta - pharmacology ; Tumor Necrosis Factor-alpha - antagonists & inhibitors ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>American journal of obstetrics and gynecology, 1993-04, Vol.168 (4), p.1318-1322</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8475982$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bry, K</creatorcontrib><creatorcontrib>Hallman, M</creatorcontrib><title>Transforming growth factor-beta 2 prevents preterm delivery induced by interleukin-1 alpha and tumor necrosis factor-alpha in the rabbit</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>The purpose of the study was to determine whether preterm parturition in the rabbit can be induced by intraamniotic injection of proinflammatory cytokines, interleukin-1 alpha and tumor necrosis factor-alpha, and whether transforming growth factor-beta 2, an inhibitor of the cytokine-induced prostaglandin synthesis, modifies the effect of these cytokines.
New Zealand White rabbits were injected in each amniotic cavity on day 24 of gestation with one of the following: a combination of interleukin-1 alpha (150 ng) and tumor necrosis factor-alpha (1.25 micrograms), 50 ng of transforming growth factor-beta 2 concomitantly with interleukin-1 alpha and tumor necrosis factor-alpha, or vehicle. In the first study the animals were observed for signs of delivery until day 29 of gestation. In the second study the effect of transforming growth factor-beta 2 (50 ng/fetus) on the rate of premature delivery was evaluated. In the third study the concentrations of prostaglandin E2 and 13,14-dihydro-15-keto-prostaglandin F2 alpha were measured in the amniotic fluid on day 27 of gestation. The statistics used were Fisher's exact test, the chi 2 test, and the Mann-Whitney U test.
Altogether 61 of 191 fetuses (32%) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha group, whereas only two of 161 fetuses (1.2%) (p = 0.0001) and one of 159 (0.6%) (p = 0.0001) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group and in the control group, respectively. Of the 23 animals injected with interleukin-1 alpha and tumor necrosis factor-alpha, six (26%) delivered all of their fetuses prematurely versus none in the other groups (p = 0.02). None of the 88 fetuses in the transforming growth factor-beta 2 group were born prematurely. The prostaglandin E2 concentrations in the amniotic fluids were higher in the interleukin-1 alpha-tumor necrosis factor-alpha group than in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group (p = 0.05) or in the control group (p = 0.02).
Preterm parturition can be provoked in the rabbit by intraamniotic injections of interleukin-1 alpha and tumor necrosis factor-alpha. Transforming growth factor-beta 2 prevents the cytokine-induced increase in premature delivery.</description><subject>Amniotic Fluid - chemistry</subject><subject>Animals</subject><subject>Dinoprost - analogs & derivatives</subject><subject>Dinoprost - analysis</subject><subject>Dinoprostone - analysis</subject><subject>Female</subject><subject>Interleukin-1 - antagonists & inhibitors</subject><subject>Interleukin-1 - pharmacology</subject><subject>Obstetric Labor, Premature - chemically induced</subject><subject>Obstetric Labor, Premature - metabolism</subject><subject>Obstetric Labor, Premature - prevention & control</subject><subject>Pregnancy</subject><subject>Progesterone - blood</subject><subject>Rabbits</subject><subject>Transforming Growth Factor beta - pharmacology</subject><subject>Tumor Necrosis Factor-alpha - antagonists & inhibitors</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0002-9378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kL1OwzAQxz2ASim8AUieEAwBX5zU9ogqvqRKLGVgipz40hoSJ9hOUd-Ax6ZVC7fc_-P0G46QC2C3wGB6xxhLE8WFvFb8RjEuZfJ-RMb_8Qk5DeFjZ1OVjshIZiJXMh2Tn4XXLtSdb61b0qXvvuOK1rqKnU9KjJqmtPe4RhfDTkT0LTXY2DX6DbXODBUaWu7ktmpw-LQuAaqbfqWpdobGoe08dVj5LtjwR9731tG4Qup1Wdp4Ro5r3QQ8P-wJeXt8WMyek_nr08vsfp70wGVMsjrLDIg6RQViKjjoSmFeQg0m56nMJIDSOZRmykAgAPKsrlSlBdeS6UzzCbnac3vffQ0YYtHaUGHTaIfdEAqRT2XKtzMhl4fDoWzRFL23rfab4vA6_gtraHGb</recordid><startdate>199304</startdate><enddate>199304</enddate><creator>Bry, K</creator><creator>Hallman, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199304</creationdate><title>Transforming growth factor-beta 2 prevents preterm delivery induced by interleukin-1 alpha and tumor necrosis factor-alpha in the rabbit</title><author>Bry, K ; Hallman, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p138t-4f44d17f2e9176731ac9e5b1f1d532848119a51bd6017e11e34fc9ca73a80a4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amniotic Fluid - chemistry</topic><topic>Animals</topic><topic>Dinoprost - analogs & derivatives</topic><topic>Dinoprost - analysis</topic><topic>Dinoprostone - analysis</topic><topic>Female</topic><topic>Interleukin-1 - antagonists & inhibitors</topic><topic>Interleukin-1 - pharmacology</topic><topic>Obstetric Labor, Premature - chemically induced</topic><topic>Obstetric Labor, Premature - metabolism</topic><topic>Obstetric Labor, Premature - prevention & control</topic><topic>Pregnancy</topic><topic>Progesterone - blood</topic><topic>Rabbits</topic><topic>Transforming Growth Factor beta - pharmacology</topic><topic>Tumor Necrosis Factor-alpha - antagonists & inhibitors</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bry, K</creatorcontrib><creatorcontrib>Hallman, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bry, K</au><au>Hallman, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transforming growth factor-beta 2 prevents preterm delivery induced by interleukin-1 alpha and tumor necrosis factor-alpha in the rabbit</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1993-04</date><risdate>1993</risdate><volume>168</volume><issue>4</issue><spage>1318</spage><epage>1322</epage><pages>1318-1322</pages><issn>0002-9378</issn><abstract>The purpose of the study was to determine whether preterm parturition in the rabbit can be induced by intraamniotic injection of proinflammatory cytokines, interleukin-1 alpha and tumor necrosis factor-alpha, and whether transforming growth factor-beta 2, an inhibitor of the cytokine-induced prostaglandin synthesis, modifies the effect of these cytokines.
New Zealand White rabbits were injected in each amniotic cavity on day 24 of gestation with one of the following: a combination of interleukin-1 alpha (150 ng) and tumor necrosis factor-alpha (1.25 micrograms), 50 ng of transforming growth factor-beta 2 concomitantly with interleukin-1 alpha and tumor necrosis factor-alpha, or vehicle. In the first study the animals were observed for signs of delivery until day 29 of gestation. In the second study the effect of transforming growth factor-beta 2 (50 ng/fetus) on the rate of premature delivery was evaluated. In the third study the concentrations of prostaglandin E2 and 13,14-dihydro-15-keto-prostaglandin F2 alpha were measured in the amniotic fluid on day 27 of gestation. The statistics used were Fisher's exact test, the chi 2 test, and the Mann-Whitney U test.
Altogether 61 of 191 fetuses (32%) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha group, whereas only two of 161 fetuses (1.2%) (p = 0.0001) and one of 159 (0.6%) (p = 0.0001) were born prematurely in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group and in the control group, respectively. Of the 23 animals injected with interleukin-1 alpha and tumor necrosis factor-alpha, six (26%) delivered all of their fetuses prematurely versus none in the other groups (p = 0.02). None of the 88 fetuses in the transforming growth factor-beta 2 group were born prematurely. The prostaglandin E2 concentrations in the amniotic fluids were higher in the interleukin-1 alpha-tumor necrosis factor-alpha group than in the interleukin-1 alpha-tumor necrosis factor-alpha-transforming growth factor-beta 2 group (p = 0.05) or in the control group (p = 0.02).
Preterm parturition can be provoked in the rabbit by intraamniotic injections of interleukin-1 alpha and tumor necrosis factor-alpha. Transforming growth factor-beta 2 prevents the cytokine-induced increase in premature delivery.</abstract><cop>United States</cop><pmid>8475982</pmid><doi>10.1016/0002-9378(93)90388-Y</doi><tpages>5</tpages></addata></record> |
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| ispartof | American journal of obstetrics and gynecology, 1993-04, Vol.168 (4), p.1318-1322 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amniotic Fluid - chemistry Animals Dinoprost - analogs & derivatives Dinoprost - analysis Dinoprostone - analysis Female Interleukin-1 - antagonists & inhibitors Interleukin-1 - pharmacology Obstetric Labor, Premature - chemically induced Obstetric Labor, Premature - metabolism Obstetric Labor, Premature - prevention & control Pregnancy Progesterone - blood Rabbits Transforming Growth Factor beta - pharmacology Tumor Necrosis Factor-alpha - antagonists & inhibitors Tumor Necrosis Factor-alpha - pharmacology |
| title | Transforming growth factor-beta 2 prevents preterm delivery induced by interleukin-1 alpha and tumor necrosis factor-alpha in the rabbit |
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