Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa
This work was designed to study how this ability is affected by different sperm treatments routinely used for in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulb...
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| Veröffentlicht in: | Theriogenology 2010-11, Vol.74 (8), p.1327-1340 |
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| creator | Matás, C. Sansegundo, M. Ruiz, S. García-Vázquez, F.A. Gadea, J. Romar, R. Coy, P. |
| description | This work was designed to study how this ability is affected by different sperm treatments routinely used for
in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with 0.1% BSA (BSA group), and washed on a Percoll
® gradient (PERCOLL group). After preparation of semen samples, changes in motility patterns were studied by CASA, calcium uptake by spectrofluorimetry, and ROS generation, spontaneous acrosome reaction, and lipid disorder by means of flow cytometry. Finally IVF assays were also performed with the different semen samples and penetrability results evaluated at 2 and 4 h post insemination (hpi). Independently of the sperm treatment, epididymal spermatozoa showed higher values of progressive motility, percentage of live cells with low lipid disorder, and penetration ability at 4 hpi than the corresponding ejaculated spermatozoa. Ejaculated spermatozoa showed higher levels of calcium uptake, ROS generation and percentage of spontaneous acrosome reaction than epididymal sperm. Regarding sperm treatments, PERCOLL group showed the highest values for some motility parameters (linearity of the curvilinear trajectory, straightness, and average path velocity/curvilinear velocity), ROS generation and penetration ability at 2 and 4 hpi; however this same group showed the lowest values for sperm curvilinear velocity and lateral head displacement. From all experimental groups, ejaculated-PERCOLL
-treated spermatozoa showed the highest fertilization ability after 2 hpi. Results suggest that capacitation pathways can be regulated by suitable treatments making the ejaculated sperm able to reach capacitation and fertilize oocytes in similar levels than epididymal spermatozoa, although most of the studied capacitation-associated changes do not correlate with this ability. |
| doi_str_mv | 10.1016/j.theriogenology.2010.06.002 |
| format | Article |
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in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with 0.1% BSA (BSA group), and washed on a Percoll
® gradient (PERCOLL group). After preparation of semen samples, changes in motility patterns were studied by CASA, calcium uptake by spectrofluorimetry, and ROS generation, spontaneous acrosome reaction, and lipid disorder by means of flow cytometry. Finally IVF assays were also performed with the different semen samples and penetrability results evaluated at 2 and 4 h post insemination (hpi). Independently of the sperm treatment, epididymal spermatozoa showed higher values of progressive motility, percentage of live cells with low lipid disorder, and penetration ability at 4 hpi than the corresponding ejaculated spermatozoa. Ejaculated spermatozoa showed higher levels of calcium uptake, ROS generation and percentage of spontaneous acrosome reaction than epididymal sperm. Regarding sperm treatments, PERCOLL group showed the highest values for some motility parameters (linearity of the curvilinear trajectory, straightness, and average path velocity/curvilinear velocity), ROS generation and penetration ability at 2 and 4 hpi; however this same group showed the lowest values for sperm curvilinear velocity and lateral head displacement. From all experimental groups, ejaculated-PERCOLL
-treated spermatozoa showed the highest fertilization ability after 2 hpi. Results suggest that capacitation pathways can be regulated by suitable treatments making the ejaculated sperm able to reach capacitation and fertilize oocytes in similar levels than epididymal spermatozoa, although most of the studied capacitation-associated changes do not correlate with this ability.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2010.06.002</identifier><identifier>PMID: 20688369</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acrosome - drug effects ; acrosome reaction ; Acrosome Reaction - drug effects ; Animals ; boars ; Calcium - metabolism ; Capacitation ; chemical treatment ; Ejaculated spermatozoa ; Ejaculation ; Epididymal spermatozoa ; epididymis ; Epididymis - cytology ; Female ; Fertilization in Vitro - veterinary ; in vitro fertilization ; In vitro penetration ; insemination ; Kinetics ; Lipid Metabolism ; Male ; oocytes ; Percoll treatment ; Reactive Oxygen Species - metabolism ; resistance to penetration ; Sperm Capacitation ; Sperm Motility ; sperm penetration ability ; Sperm-Ovum Interactions - drug effects ; spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - metabolism ; Spermatozoa - physiology ; spermatozoa treatment ; Swine - physiology</subject><ispartof>Theriogenology, 2010-11, Vol.74 (8), p.1327-1340</ispartof><rights>2010 Elsevier Inc.</rights><rights>Copyright © 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-792c3424a3b1fbb3aed8d10754730974a2940f1b8c0f93af004b87cd51d110d63</citedby><cites>FETCH-LOGICAL-c475t-792c3424a3b1fbb3aed8d10754730974a2940f1b8c0f93af004b87cd51d110d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2010.06.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20688369$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matás, C.</creatorcontrib><creatorcontrib>Sansegundo, M.</creatorcontrib><creatorcontrib>Ruiz, S.</creatorcontrib><creatorcontrib>García-Vázquez, F.A.</creatorcontrib><creatorcontrib>Gadea, J.</creatorcontrib><creatorcontrib>Romar, R.</creatorcontrib><creatorcontrib>Coy, P.</creatorcontrib><title>Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>This work was designed to study how this ability is affected by different sperm treatments routinely used for
in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with 0.1% BSA (BSA group), and washed on a Percoll
® gradient (PERCOLL group). After preparation of semen samples, changes in motility patterns were studied by CASA, calcium uptake by spectrofluorimetry, and ROS generation, spontaneous acrosome reaction, and lipid disorder by means of flow cytometry. Finally IVF assays were also performed with the different semen samples and penetrability results evaluated at 2 and 4 h post insemination (hpi). Independently of the sperm treatment, epididymal spermatozoa showed higher values of progressive motility, percentage of live cells with low lipid disorder, and penetration ability at 4 hpi than the corresponding ejaculated spermatozoa. Ejaculated spermatozoa showed higher levels of calcium uptake, ROS generation and percentage of spontaneous acrosome reaction than epididymal sperm. Regarding sperm treatments, PERCOLL group showed the highest values for some motility parameters (linearity of the curvilinear trajectory, straightness, and average path velocity/curvilinear velocity), ROS generation and penetration ability at 2 and 4 hpi; however this same group showed the lowest values for sperm curvilinear velocity and lateral head displacement. From all experimental groups, ejaculated-PERCOLL
-treated spermatozoa showed the highest fertilization ability after 2 hpi. Results suggest that capacitation pathways can be regulated by suitable treatments making the ejaculated sperm able to reach capacitation and fertilize oocytes in similar levels than epididymal spermatozoa, although most of the studied capacitation-associated changes do not correlate with this ability.</description><subject>Acrosome - drug effects</subject><subject>acrosome reaction</subject><subject>Acrosome Reaction - drug effects</subject><subject>Animals</subject><subject>boars</subject><subject>Calcium - metabolism</subject><subject>Capacitation</subject><subject>chemical treatment</subject><subject>Ejaculated spermatozoa</subject><subject>Ejaculation</subject><subject>Epididymal spermatozoa</subject><subject>epididymis</subject><subject>Epididymis - cytology</subject><subject>Female</subject><subject>Fertilization in Vitro - veterinary</subject><subject>in vitro fertilization</subject><subject>In vitro penetration</subject><subject>insemination</subject><subject>Kinetics</subject><subject>Lipid Metabolism</subject><subject>Male</subject><subject>oocytes</subject><subject>Percoll treatment</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>resistance to penetration</subject><subject>Sperm Capacitation</subject><subject>Sperm Motility</subject><subject>sperm penetration ability</subject><subject>Sperm-Ovum Interactions - drug effects</subject><subject>spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><subject>Spermatozoa - physiology</subject><subject>spermatozoa treatment</subject><subject>Swine - physiology</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2L1TAUhosoznX0L2gXgqteT5o2acCNDI4KAy7GAXfhNDm55tI2NUkHrr_eXjsK7lydxfucD55TFK8Z7Bkw8fa4z98p-nCgKQzhcNrXsEYg9gD1o2LHOqkqXnP2uNgBKF4Jxb5dFM9SOgIAF4I9LS5qEF3HhdoVy-1McSxzJMwjTblE58jkVBqc0fiM2YepnDHiSJliKnGy5UwT5bhF2PvB51MZXElHNMuAmexvimZvvT2NOJR9wFim8ybM4WfA58UTh0OiFw_1sri7_vD16lN18-Xj56v3N5VpZJsrqWrDm7pB3jPX9xzJdpaBbBvJQckGa9WAY31nwCmODqDpO2lsyyxjYAW_LN5sc-cYfiyUsh59MjQMOFFYkpat6OpaqTP5biNNDClFcnqOfsR40gz02bs-6n-967N3DUKv3tf2lw-Lln4k-7f5j-gVeLUBDoPGQ_RJ392uEziwTknZditxvRG0Crn3FHUyniZD1sf1I9oG_3-3_AJVGak3</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Matás, C.</creator><creator>Sansegundo, M.</creator><creator>Ruiz, S.</creator><creator>García-Vázquez, F.A.</creator><creator>Gadea, J.</creator><creator>Romar, R.</creator><creator>Coy, P.</creator><general>Elsevier Inc</general><general>[Oxford]: Butterworth-Heinemann; [New York]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa</title><author>Matás, C. ; Sansegundo, M. ; Ruiz, S. ; García-Vázquez, F.A. ; Gadea, J. ; Romar, R. ; Coy, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-792c3424a3b1fbb3aed8d10754730974a2940f1b8c0f93af004b87cd51d110d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acrosome - drug effects</topic><topic>acrosome reaction</topic><topic>Acrosome Reaction - drug effects</topic><topic>Animals</topic><topic>boars</topic><topic>Calcium - metabolism</topic><topic>Capacitation</topic><topic>chemical treatment</topic><topic>Ejaculated spermatozoa</topic><topic>Ejaculation</topic><topic>Epididymal spermatozoa</topic><topic>epididymis</topic><topic>Epididymis - cytology</topic><topic>Female</topic><topic>Fertilization in Vitro - veterinary</topic><topic>in vitro fertilization</topic><topic>In vitro penetration</topic><topic>insemination</topic><topic>Kinetics</topic><topic>Lipid Metabolism</topic><topic>Male</topic><topic>oocytes</topic><topic>Percoll treatment</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>resistance to penetration</topic><topic>Sperm Capacitation</topic><topic>Sperm Motility</topic><topic>sperm penetration ability</topic><topic>Sperm-Ovum Interactions - drug effects</topic><topic>spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><topic>Spermatozoa - physiology</topic><topic>spermatozoa treatment</topic><topic>Swine - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matás, C.</creatorcontrib><creatorcontrib>Sansegundo, M.</creatorcontrib><creatorcontrib>Ruiz, S.</creatorcontrib><creatorcontrib>García-Vázquez, F.A.</creatorcontrib><creatorcontrib>Gadea, J.</creatorcontrib><creatorcontrib>Romar, R.</creatorcontrib><creatorcontrib>Coy, P.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matás, C.</au><au>Sansegundo, M.</au><au>Ruiz, S.</au><au>García-Vázquez, F.A.</au><au>Gadea, J.</au><au>Romar, R.</au><au>Coy, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>74</volume><issue>8</issue><spage>1327</spage><epage>1340</epage><pages>1327-1340</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>This work was designed to study how this ability is affected by different sperm treatments routinely used for
in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with 0.1% BSA (BSA group), and washed on a Percoll
® gradient (PERCOLL group). After preparation of semen samples, changes in motility patterns were studied by CASA, calcium uptake by spectrofluorimetry, and ROS generation, spontaneous acrosome reaction, and lipid disorder by means of flow cytometry. Finally IVF assays were also performed with the different semen samples and penetrability results evaluated at 2 and 4 h post insemination (hpi). Independently of the sperm treatment, epididymal spermatozoa showed higher values of progressive motility, percentage of live cells with low lipid disorder, and penetration ability at 4 hpi than the corresponding ejaculated spermatozoa. Ejaculated spermatozoa showed higher levels of calcium uptake, ROS generation and percentage of spontaneous acrosome reaction than epididymal sperm. Regarding sperm treatments, PERCOLL group showed the highest values for some motility parameters (linearity of the curvilinear trajectory, straightness, and average path velocity/curvilinear velocity), ROS generation and penetration ability at 2 and 4 hpi; however this same group showed the lowest values for sperm curvilinear velocity and lateral head displacement. From all experimental groups, ejaculated-PERCOLL
-treated spermatozoa showed the highest fertilization ability after 2 hpi. Results suggest that capacitation pathways can be regulated by suitable treatments making the ejaculated sperm able to reach capacitation and fertilize oocytes in similar levels than epididymal spermatozoa, although most of the studied capacitation-associated changes do not correlate with this ability.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20688369</pmid><doi>10.1016/j.theriogenology.2010.06.002</doi><tpages>14</tpages></addata></record> |
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| subjects | Acrosome - drug effects acrosome reaction Acrosome Reaction - drug effects Animals boars Calcium - metabolism Capacitation chemical treatment Ejaculated spermatozoa Ejaculation Epididymal spermatozoa epididymis Epididymis - cytology Female Fertilization in Vitro - veterinary in vitro fertilization In vitro penetration insemination Kinetics Lipid Metabolism Male oocytes Percoll treatment Reactive Oxygen Species - metabolism resistance to penetration Sperm Capacitation Sperm Motility sperm penetration ability Sperm-Ovum Interactions - drug effects spermatozoa Spermatozoa - drug effects Spermatozoa - metabolism Spermatozoa - physiology spermatozoa treatment Swine - physiology |
| title | Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa |
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