Electric stimulation regulates the level of Ca-channels in chick muscle culture

To examine the role of muscle activity in the expression of 1,4-dihydropyridine (DHP)-sensitive Ca-channels, we electrically stimulated chick skeletal muscle cells growing in culture. The number of Ca-channels was determined by a radioligand binding technique using [ 3H]PN200-110. The function of th...

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Veröffentlicht in:Neuroscience letters 1993-03, Vol.151 (1), p.104-106
Hauptverfasser: Freud-Silverberg, Michal, Shainberg, Asher
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container_title Neuroscience letters
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creator Freud-Silverberg, Michal
Shainberg, Asher
description To examine the role of muscle activity in the expression of 1,4-dihydropyridine (DHP)-sensitive Ca-channels, we electrically stimulated chick skeletal muscle cells growing in culture. The number of Ca-channels was determined by a radioligand binding technique using [ 3H]PN200-110. The function of these channels was measured by depolarization-induced 45Ca uptake. We found that both the amount and the function of these channels were higher as a result of the increased muscle activity caused by electrical stimulation (ES).
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source MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects Acetylcholinesterase - metabolism
Animals
Biological and medical sciences
Ca-channel
Calcium Channels - drug effects
Calcium Channels - metabolism
Calcium Channels - physiology
Chick Embryo
Culture Techniques
DHP receptor
Electric Stimulation
Fundamental and applied biological sciences. Psychology
Isradipine - metabolism
Muscle culture
Muscles - enzymology
Muscles - metabolism
Muscles - physiology
Potassium - pharmacology
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
title Electric stimulation regulates the level of Ca-channels in chick muscle culture
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