Electric stimulation regulates the level of Ca-channels in chick muscle culture
To examine the role of muscle activity in the expression of 1,4-dihydropyridine (DHP)-sensitive Ca-channels, we electrically stimulated chick skeletal muscle cells growing in culture. The number of Ca-channels was determined by a radioligand binding technique using [ 3H]PN200-110. The function of th...
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Veröffentlicht in: | Neuroscience letters 1993-03, Vol.151 (1), p.104-106 |
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creator | Freud-Silverberg, Michal Shainberg, Asher |
description | To examine the role of muscle activity in the expression of 1,4-dihydropyridine (DHP)-sensitive Ca-channels, we electrically stimulated chick skeletal muscle cells growing in culture. The number of Ca-channels was determined by a radioligand binding technique using [
3H]PN200-110. The function of these channels was measured by depolarization-induced
45Ca uptake. We found that both the amount and the function of these channels were higher as a result of the increased muscle activity caused by electrical stimulation (ES). |
doi_str_mv | 10.1016/0304-3940(93)90057-R |
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3H]PN200-110. The function of these channels was measured by depolarization-induced
45Ca uptake. We found that both the amount and the function of these channels were higher as a result of the increased muscle activity caused by electrical stimulation (ES).</description><subject>Acetylcholinesterase - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Ca-channel</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Channels - physiology</subject><subject>Chick Embryo</subject><subject>Culture Techniques</subject><subject>DHP receptor</subject><subject>Electric Stimulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isradipine - metabolism</subject><subject>Muscle culture</subject><subject>Muscles - enzymology</subject><subject>Muscles - metabolism</subject><subject>Muscles - physiology</subject><subject>Potassium - pharmacology</subject><subject>Striated muscle. Tendons</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFrFDEQx4Mo9Vr9Bgp5ENGHbZPN5JK8FORoVSgUSn0OcXbWi2Z3a7Jb6Lc35x33qE8J-f9mmPmFsTdSnEsh1xdCCWiUA_HBqY9OCG2au2dsJa1pG-NM-5ytjshLdlrKT1EhqeGEnVhltdHrFbu9SoRzjsjLHIclhTlOI8_0Y3elwuct8USPlPjU801ocBvGkVLhceS4jfiLD0vBRByXNC-ZXrEXfUiFXh_OM_bt-up-86W5uf38dfPppkGQZm5013YygJMAAlAYAJDStq6zva1vbSs1QofaknWqFb1BCC3qte5bZWwX1Bl7v-_7kKffC5XZD7EgpRRGmpbi63JWOgv_BeVaAyijKgh7EPNUSqbeP-Q4hPzkpfA74X5n0-9seqf8X-H-rpa9PfRfvg_UHYsOhmv-7pCHgiH1OYwYyxEDI2x1ULHLPVbd0mOk7AtGGpG6mOsH-W6K_57jD8zDmqM</recordid><startdate>19930305</startdate><enddate>19930305</enddate><creator>Freud-Silverberg, Michal</creator><creator>Shainberg, Asher</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19930305</creationdate><title>Electric stimulation regulates the level of Ca-channels in chick muscle culture</title><author>Freud-Silverberg, Michal ; Shainberg, Asher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-5d2d1a4914404c0744411829d8f81442215c4dc58e89320f7c4a2c565f2378da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetylcholinesterase - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Ca-channel</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Channels - physiology</topic><topic>Chick Embryo</topic><topic>Culture Techniques</topic><topic>DHP receptor</topic><topic>Electric Stimulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isradipine - metabolism</topic><topic>Muscle culture</topic><topic>Muscles - enzymology</topic><topic>Muscles - metabolism</topic><topic>Muscles - physiology</topic><topic>Potassium - pharmacology</topic><topic>Striated muscle. Tendons</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freud-Silverberg, Michal</creatorcontrib><creatorcontrib>Shainberg, Asher</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freud-Silverberg, Michal</au><au>Shainberg, Asher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electric stimulation regulates the level of Ca-channels in chick muscle culture</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1993-03-05</date><risdate>1993</risdate><volume>151</volume><issue>1</issue><spage>104</spage><epage>106</epage><pages>104-106</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>To examine the role of muscle activity in the expression of 1,4-dihydropyridine (DHP)-sensitive Ca-channels, we electrically stimulated chick skeletal muscle cells growing in culture. The number of Ca-channels was determined by a radioligand binding technique using [
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subjects | Acetylcholinesterase - metabolism Animals Biological and medical sciences Ca-channel Calcium Channels - drug effects Calcium Channels - metabolism Calcium Channels - physiology Chick Embryo Culture Techniques DHP receptor Electric Stimulation Fundamental and applied biological sciences. Psychology Isradipine - metabolism Muscle culture Muscles - enzymology Muscles - metabolism Muscles - physiology Potassium - pharmacology Striated muscle. Tendons Vertebrates: osteoarticular system, musculoskeletal system |
title | Electric stimulation regulates the level of Ca-channels in chick muscle culture |
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