Bovine rotavirus type detection by neutralizing monoclonal antibodies
A series of monoclonal antibodies were developed against bovine rotavirus Q17. Among the five high affinity antibodies characterized, two (RQ 31 and RQ 4) were able to neutralize type G 6 viruses and may be specific for PB 1 type virus. Seventy seven feces from diarrheic calves were tested by "...
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Veröffentlicht in: | Archives of virology 1993-01, Vol.129 (1/4), p.243-250 |
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creator | Cornaglia, E Elazhary, Y Talbot, B |
description | A series of monoclonal antibodies were developed against bovine rotavirus Q17. Among the five high affinity antibodies characterized, two (RQ 31 and RQ 4) were able to neutralize type G 6 viruses and may be specific for PB 1 type virus. Seventy seven feces from diarrheic calves were tested by "double sandwich" ELISA using four monoclonal and one polyclonal anti-rotavirus antibodies. The combination of mono- and polyclonal antibodies thus appears to be a more efficient strategy for detection and typing of bovine rotaviruses. |
doi_str_mv | 10.1007/BF01316899 |
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Among the five high affinity antibodies characterized, two (RQ 31 and RQ 4) were able to neutralize type G 6 viruses and may be specific for PB 1 type virus. Seventy seven feces from diarrheic calves were tested by "double sandwich" ELISA using four monoclonal and one polyclonal anti-rotavirus antibodies. The combination of mono- and polyclonal antibodies thus appears to be a more efficient strategy for detection and typing of bovine rotaviruses.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/BF01316899</identifier><identifier>PMID: 7682402</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antigens, Viral ; Biological and medical sciences ; Capsid - immunology ; Capsid Proteins ; Cattle ; Enzyme-Linked Immunosorbent Assay ; Epitopes - analysis ; Fundamental and applied biological sciences. 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Among the five high affinity antibodies characterized, two (RQ 31 and RQ 4) were able to neutralize type G 6 viruses and may be specific for PB 1 type virus. Seventy seven feces from diarrheic calves were tested by "double sandwich" ELISA using four monoclonal and one polyclonal anti-rotavirus antibodies. The combination of mono- and polyclonal antibodies thus appears to be a more efficient strategy for detection and typing of bovine rotaviruses.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Viral</subject><subject>Biological and medical sciences</subject><subject>Capsid - immunology</subject><subject>Capsid Proteins</subject><subject>Cattle</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>monoclonal antibodies</subject><subject>Neutralization Tests</subject><subject>Rotavirus</subject><subject>Rotavirus - classification</subject><subject>Rotavirus - immunology</subject><subject>Rotavirus - isolation & purification</subject><subject>Swine - microbiology</subject><subject>Techniques used in virology</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MtLxDAQBvAgyro-Lt7FHsSDUJ00zx518QWCB91zSdPpEukma9IK619vZRc9ehqY-fENfIScULiiAOr69h4oo1KX5Q6ZUs6KXKtS75IpMOC5lqD3yUFK7wDjgokJmSipCw7FlNzdhk_nMYuhN58uDinr1yvMGuzR9i74rF5nHoc-ms59Ob_IlsEH2wVvusz43tWhcZiOyF5ruoTH23lI5vd3b7PH_Pnl4Wl285zb8Vmfq0a2iG3bYAFCa2goY6ouraBSKSaYFdBqLQwCllbxRhlVYM1tI2VZgxDskFxsclcxfAyY-mrpksWuMx7DkColpNJU8n8hVaCoZuUILzfQxpBSxLZaRbc0cV1RqH7Krf7KHfHpNnWol9j80m2b4_18ezfJmq6NxluXfhmXXJVKj-xsw1oTKrOII5m_FuMXoFwXlAP7Bs8XieM</recordid><startdate>19930101</startdate><enddate>19930101</enddate><creator>Cornaglia, E</creator><creator>Elazhary, Y</creator><creator>Talbot, B</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19930101</creationdate><title>Bovine rotavirus type detection by neutralizing monoclonal antibodies</title><author>Cornaglia, E ; Elazhary, Y ; Talbot, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-7d6feeffde205880d1337b9c51677353c50f885ae0e9c74d7a72eb4cd669b0553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Viral</topic><topic>Biological and medical sciences</topic><topic>Capsid - immunology</topic><topic>Capsid Proteins</topic><topic>Cattle</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>monoclonal antibodies</topic><topic>Neutralization Tests</topic><topic>Rotavirus</topic><topic>Rotavirus - classification</topic><topic>Rotavirus - immunology</topic><topic>Rotavirus - isolation & purification</topic><topic>Swine - microbiology</topic><topic>Techniques used in virology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cornaglia, E</creatorcontrib><creatorcontrib>Elazhary, Y</creatorcontrib><creatorcontrib>Talbot, B</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cornaglia, E</au><au>Elazhary, Y</au><au>Talbot, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine rotavirus type detection by neutralizing monoclonal antibodies</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1993-01-01</date><risdate>1993</risdate><volume>129</volume><issue>1/4</issue><spage>243</spage><epage>250</epage><pages>243-250</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>A series of monoclonal antibodies were developed against bovine rotavirus Q17. 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subjects | Animals Antibodies, Monoclonal - immunology Antigens, Viral Biological and medical sciences Capsid - immunology Capsid Proteins Cattle Enzyme-Linked Immunosorbent Assay Epitopes - analysis Fundamental and applied biological sciences. Psychology Mice Mice, Inbred BALB C Microbiology monoclonal antibodies Neutralization Tests Rotavirus Rotavirus - classification Rotavirus - immunology Rotavirus - isolation & purification Swine - microbiology Techniques used in virology Virology |
title | Bovine rotavirus type detection by neutralizing monoclonal antibodies |
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