Formation of hydroxypyruvaldehyde phosphate in human erythrocytes
Human erythrocytes when incubated with [U-14C]glucose formed radioactively labeled hydroxypyruvaldehyde-P (CHOCOCH2OPO3(2-)). Using a "diagonal" chromatographic procedure on an anion exchange column for isolation and quantitative detection, up to 0.3 mumol of hydroxypyruvaldehyde-P/liter o...
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| Veröffentlicht in: | The Journal of biological chemistry 1981-06, Vol.256 (11), p.5708-5711 |
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| container_title | The Journal of biological chemistry |
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| creator | Cogoli-Greuter, M Christen, P |
| description | Human erythrocytes when incubated with [U-14C]glucose formed radioactively labeled hydroxypyruvaldehyde-P (CHOCOCH2OPO3(2-)).
Using a "diagonal" chromatographic procedure on an anion exchange column for isolation and quantitative detection, up to 0.3
mumol of hydroxypyruvaldehyde-P/liter of packed cells was found. If glycolysis was blocked by inhibition of glyceraldehyde-3-P
dehydrogenase with N-ethylmaleimide and iodoacetate, 0.7 mumol of hydroxypyruvaldehyde-P/liter of packed erythrocytes was
detected. Hydroxypyruvaldehyde-P, found for the first time in eukaryotic cells, might be one of the long sought natural substrates
of the glyoxalases. |
| doi_str_mv | 10.1016/S0021-9258(19)69263-X |
| format | Article |
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Using a "diagonal" chromatographic procedure on an anion exchange column for isolation and quantitative detection, up to 0.3
mumol of hydroxypyruvaldehyde-P/liter of packed cells was found. If glycolysis was blocked by inhibition of glyceraldehyde-3-P
dehydrogenase with N-ethylmaleimide and iodoacetate, 0.7 mumol of hydroxypyruvaldehyde-P/liter of packed erythrocytes was
detected. Hydroxypyruvaldehyde-P, found for the first time in eukaryotic cells, might be one of the long sought natural substrates
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Using a "diagonal" chromatographic procedure on an anion exchange column for isolation and quantitative detection, up to 0.3
mumol of hydroxypyruvaldehyde-P/liter of packed cells was found. If glycolysis was blocked by inhibition of glyceraldehyde-3-P
dehydrogenase with N-ethylmaleimide and iodoacetate, 0.7 mumol of hydroxypyruvaldehyde-P/liter of packed erythrocytes was
detected. Hydroxypyruvaldehyde-P, found for the first time in eukaryotic cells, might be one of the long sought natural substrates
of the glyoxalases.</description><subject>Aldehydes - blood</subject><subject>Blood Glucose - metabolism</subject><subject>Carbon Radioisotopes</subject><subject>Erythrocytes - metabolism</subject><subject>Glyoxal - analogs & derivatives</subject><subject>Glyoxal - biosynthesis</subject><subject>Glyoxal - blood</subject><subject>Kinetics</subject><subject>Organophosphorus Compounds - biosynthesis</subject><subject>Organophosphorus Compounds - blood</subject><subject>Radioisotope Dilution Technique</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1LwzAUhoMoc05_wqAgiF5Uk6ZJmssxnAoDL1TYXUjTU1tpm5q0av-93Qc7N4fD-3HgQWhO8D3BhD-8YRyRUEYsuSXyjsuI03BzgqYEJzSkjGxO0fRoOUcX3n_hcWJJJmgixoaEySlarKyrdVfaJrB5UAyZs39DO7j-R1cZjDcEbWF9W-gOgrIJir7WTQBu6ApnzdCBv0Rnua48XB32DH2sHt-Xz-H69elluViHhgrZhZoDNpKzlBgtIdY8jRMuaIZxQrKccpIDzViUMyGTyMRS5JkBLAyhMdcpz-kM3ex7W2e_e_CdqktvoKp0A7b3SjAumBTxaGR7o3HWewe5al1ZazcogtUWndqhU1suiki1Q6c2Y25-eNCnNWTH1IHVqF_v9aL8LH5LByotrSmgVhHjihDFxIj-H6OZdu8</recordid><startdate>19810610</startdate><enddate>19810610</enddate><creator>Cogoli-Greuter, M</creator><creator>Christen, P</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810610</creationdate><title>Formation of hydroxypyruvaldehyde phosphate in human erythrocytes</title><author>Cogoli-Greuter, M ; Christen, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-a6e0c965b1ca9e4a6b48673d0081df361fe3d52f57982c497fdce07c1346ab6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Aldehydes - blood</topic><topic>Blood Glucose - metabolism</topic><topic>Carbon Radioisotopes</topic><topic>Erythrocytes - metabolism</topic><topic>Glyoxal - analogs & derivatives</topic><topic>Glyoxal - biosynthesis</topic><topic>Glyoxal - blood</topic><topic>Kinetics</topic><topic>Organophosphorus Compounds - biosynthesis</topic><topic>Organophosphorus Compounds - blood</topic><topic>Radioisotope Dilution Technique</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cogoli-Greuter, M</creatorcontrib><creatorcontrib>Christen, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cogoli-Greuter, M</au><au>Christen, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formation of hydroxypyruvaldehyde phosphate in human erythrocytes</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1981-06-10</date><risdate>1981</risdate><volume>256</volume><issue>11</issue><spage>5708</spage><epage>5711</epage><pages>5708-5711</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Human erythrocytes when incubated with [U-14C]glucose formed radioactively labeled hydroxypyruvaldehyde-P (CHOCOCH2OPO3(2-)).
Using a "diagonal" chromatographic procedure on an anion exchange column for isolation and quantitative detection, up to 0.3
mumol of hydroxypyruvaldehyde-P/liter of packed cells was found. If glycolysis was blocked by inhibition of glyceraldehyde-3-P
dehydrogenase with N-ethylmaleimide and iodoacetate, 0.7 mumol of hydroxypyruvaldehyde-P/liter of packed erythrocytes was
detected. Hydroxypyruvaldehyde-P, found for the first time in eukaryotic cells, might be one of the long sought natural substrates
of the glyoxalases.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>7016859</pmid><doi>10.1016/S0021-9258(19)69263-X</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1981-06, Vol.256 (11), p.5708-5711 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75675974 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aldehydes - blood Blood Glucose - metabolism Carbon Radioisotopes Erythrocytes - metabolism Glyoxal - analogs & derivatives Glyoxal - biosynthesis Glyoxal - blood Kinetics Organophosphorus Compounds - biosynthesis Organophosphorus Compounds - blood Radioisotope Dilution Technique |
| title | Formation of hydroxypyruvaldehyde phosphate in human erythrocytes |
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