Properties of soluble DNA polymerase from sera of hepatitis B virus carriers

A soluble DNA polymerase was purified 8,000-fold from hepatitis B surface antigen positive serum. The molecular weight of the enzyme by gel filtration was about 1.60 X 10(5), the sedimentation coefficient was 5.5S, the apparent Km for dTTP was 4 micrometer, the optimum pH in the presence of Mg2+ was...

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Veröffentlicht in:	Journal of medical virology 1981, Vol.6 (4), p.285-299
Hauptverfasser:	Mao, J C, Otis, E R, Mushahwar, I K, Overby, L R
Format:	Artikel
Sprache:	eng
Schlagworte:	Carrier State - enzymology DNA-Directed DNA Polymerase - analysis DNA-Directed DNA Polymerase - blood DNA-Directed DNA Polymerase - isolation & purification Hepatitis B - enzymology Hepatitis B - immunology Hepatitis B Surface Antigens - analysis Humans Isoelectric Point Molecular Weight Solubility
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