Serotonergic and peptidergic projections to the spinal cord demonstrated by a combined retrograde HRP histochemical and immunocytochemical staining method
A simple technique is described for simultaneously visualizing the cells of origin of descending brain stem-spinal systems and immunocytochemically defined neurotransmitters. The combination of horseradish peroxidase (HRP) histochemistry, enhanced by the use of CoCl 2, with the unlabeled antibody, p...
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| Veröffentlicht in: | Brain research 1981-05, Vol.211 (2), p.412-417 |
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| container_end_page | 417 |
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| container_title | Brain research |
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| creator | Bowker, R.M. Steinbusch, H.W.M. Coulter, J.D. |
| description | A simple technique is described for simultaneously visualizing the cells of origin of descending brain stem-spinal systems and immunocytochemically defined neurotransmitters. The combination of horseradish peroxidase (HRP) histochemistry, enhanced by the use of CoCl
2, with the unlabeled antibody, peroxidase-antiperoxidase (PAP) immunocytochemical staining method, revealed double labeled cells containing black punctate HRP granules within a homogeneous brown cytoplasm stained positively for serotonin (5-HT), substance P or enkephalin-like immunoreactivity. The advantages of this method over other double labeling techniques are discussed. |
| doi_str_mv | 10.1016/0006-8993(81)90965-3 |
| format | Article |
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2, with the unlabeled antibody, peroxidase-antiperoxidase (PAP) immunocytochemical staining method, revealed double labeled cells containing black punctate HRP granules within a homogeneous brown cytoplasm stained positively for serotonin (5-HT), substance P or enkephalin-like immunoreactivity. The advantages of this method over other double labeling techniques are discussed.</description><subject>Animals</subject><subject>Axonal Transport</subject><subject>Brain Stem - physiology</subject><subject>bulbospinal system</subject><subject>cobalt chloride</subject><subject>Efferent Pathways - physiology</subject><subject>Endorphins - analysis</subject><subject>enkephalin-like immunoreactivity</subject><subject>Enkephalins - analysis</subject><subject>Horseradish Peroxidase</subject><subject>Immunoenzyme Techniques</subject><subject>Raphe Nuclei - physiology</subject><subject>Rats</subject><subject>serotonin</subject><subject>Serotonin - analysis</subject><subject>Spinal Cord - physiology</subject><subject>substance P</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU2LFDEQDeKyjqv_QCEn0UOvyaSTTi7CsqgrLLj4cQ7ppHomyyRpk4wwf8Vfu2lnWDx5Kl7Ve1XUewi9ouSSEireE0JEJ5VibyV9p4gSvGNP0IrKYd2JdU-eotUj5Rl6Xsp9g4wpco7OBRW8Z2KF_nyHnGqKkDfeYhMdnmGu3h3xnNM92OpTLLgmXLeAy-yj2WGbssMOQpvUbCo4PB6wae0w-thQhprTJhsH-ObbHd76UpPdQvC2iZczPoR9TPbwT7tU46OPGxygbpN7gc4msyvw8lQv0M9PH39c33S3Xz9_ub667SwTQ-1G3vc9cZLQyQwAduCcKyHNNDE-TMrJ0Y7gzFqqVu2kQHLajwNlUk5WGcsu0Jvj3vbtrz2UqoMvFnY7EyHtix64GBjnrBH7I9HmVEqGSc_ZB5MPmhK9RKIXv_Xit5ZU_41EL7LXp_37MYB7FJ0yaPMPxzm0J397yLpYD9GC87mZr13y_z_wANnooDE</recordid><startdate>19810504</startdate><enddate>19810504</enddate><creator>Bowker, R.M.</creator><creator>Steinbusch, H.W.M.</creator><creator>Coulter, J.D.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810504</creationdate><title>Serotonergic and peptidergic projections to the spinal cord demonstrated by a combined retrograde HRP histochemical and immunocytochemical staining method</title><author>Bowker, R.M. ; Steinbusch, H.W.M. ; Coulter, J.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-b54440d801fa7eec7555968aff357f9d8bcbeda289cbecf9e8514b71388fc9ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Axonal Transport</topic><topic>Brain Stem - physiology</topic><topic>bulbospinal system</topic><topic>cobalt chloride</topic><topic>Efferent Pathways - physiology</topic><topic>Endorphins - analysis</topic><topic>enkephalin-like immunoreactivity</topic><topic>Enkephalins - analysis</topic><topic>Horseradish Peroxidase</topic><topic>Immunoenzyme Techniques</topic><topic>Raphe Nuclei - physiology</topic><topic>Rats</topic><topic>serotonin</topic><topic>Serotonin - analysis</topic><topic>Spinal Cord - physiology</topic><topic>substance P</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bowker, R.M.</creatorcontrib><creatorcontrib>Steinbusch, H.W.M.</creatorcontrib><creatorcontrib>Coulter, J.D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bowker, R.M.</au><au>Steinbusch, H.W.M.</au><au>Coulter, J.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serotonergic and peptidergic projections to the spinal cord demonstrated by a combined retrograde HRP histochemical and immunocytochemical staining method</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1981-05-04</date><risdate>1981</risdate><volume>211</volume><issue>2</issue><spage>412</spage><epage>417</epage><pages>412-417</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>A simple technique is described for simultaneously visualizing the cells of origin of descending brain stem-spinal systems and immunocytochemically defined neurotransmitters. The combination of horseradish peroxidase (HRP) histochemistry, enhanced by the use of CoCl
2, with the unlabeled antibody, peroxidase-antiperoxidase (PAP) immunocytochemical staining method, revealed double labeled cells containing black punctate HRP granules within a homogeneous brown cytoplasm stained positively for serotonin (5-HT), substance P or enkephalin-like immunoreactivity. The advantages of this method over other double labeling techniques are discussed.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6165436</pmid><doi>10.1016/0006-8993(81)90965-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Brain research, 1981-05, Vol.211 (2), p.412-417 |
| issn | 0006-8993 1872-6240 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Axonal Transport Brain Stem - physiology bulbospinal system cobalt chloride Efferent Pathways - physiology Endorphins - analysis enkephalin-like immunoreactivity Enkephalins - analysis Horseradish Peroxidase Immunoenzyme Techniques Raphe Nuclei - physiology Rats serotonin Serotonin - analysis Spinal Cord - physiology substance P |
| title | Serotonergic and peptidergic projections to the spinal cord demonstrated by a combined retrograde HRP histochemical and immunocytochemical staining method |
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