Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants
Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines defic...
Gespeichert in:
| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1993-04, Vol.260 (5105), p.207-210 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 210 |
|---|---|
| container_issue | 5105 |
| container_start_page | 207 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 260 |
| creator | Taccioli, Guillermo E. Rathbun, Gary Oltz, Eugene Stamato, Thomas Jeggo, Penny A. Alt, Frederick W. |
| description | Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines deficient in DNA repair were tested for the ability to perform V(D)J recombination after introduction of the RAG-1 and RAG-2 genes. Two mutants failed to generate normal V(D)J recombination, and further analysis provided evidence for two distinct nonlymphoid-specific genes that encode factors involved in both DNA repair and V(D)J recombination. |
| doi_str_mv | 10.1126/science.8469973 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_75670210</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>2881308</jstor_id><sourcerecordid>2881308</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-3c0bf6b852db88fbb04a88d59ee636455ab3c3f9e185d1ca630c35f6e3f155d43</originalsourceid><addsrcrecordid>eNqF0DtPwzAUBWALgUopzCwgZUAIhrR2HLv2CC2PoiIkXmtkO7bkksTFdgb-PakalZHpDue790oHgFMExwhldBKU1Y3SY5ZTzqd4DwwR5CTlGcT7YAghpimDU3IIjkJYQdhlHA_AoOdD8LCo18L6WjcxcSb5vJpfPyWvWrla2kZE65rENsnctbLS6Vv0oimTW6_FV4c2i8lzG0UTwzE4MKIK-qSfI_Bxf_c-e0yXLw-L2c0yVd3HmGIFpaGSkayUjBkpYS4YKwnXmmKaEyIkVthwjRgpkRIUQ4WJoRobREiZ4xG43N5de_fd6hCL2galq0o02rWhmBI6hRmC_0JE85wzsoGTLVTeheC1Kdbe1sL_FAgWm46LvuOiL63bOO9Pt7LW5c7_5Rd9LoISlelKUzbsWKcgz1jHzrZsFaLzuzhjDGHI8C8qdY37</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16449850</pqid></control><display><type>article</type><title>Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>American Association for the Advancement of Science</source><creator>Taccioli, Guillermo E. ; Rathbun, Gary ; Oltz, Eugene ; Stamato, Thomas ; Jeggo, Penny A. ; Alt, Frederick W.</creator><creatorcontrib>Taccioli, Guillermo E. ; Rathbun, Gary ; Oltz, Eugene ; Stamato, Thomas ; Jeggo, Penny A. ; Alt, Frederick W.</creatorcontrib><description>Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines deficient in DNA repair were tested for the ability to perform V(D)J recombination after introduction of the RAG-1 and RAG-2 genes. Two mutants failed to generate normal V(D)J recombination, and further analysis provided evidence for two distinct nonlymphoid-specific genes that encode factors involved in both DNA repair and V(D)J recombination.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.8469973</identifier><identifier>PMID: 8469973</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cell Line ; Cell lines ; CHO Cells ; Cricetinae ; DNA Nucleotidyltransferases - genetics ; DNA Nucleotidyltransferases - metabolism ; DNA Repair ; Fundamental and applied biological sciences. Psychology ; Gene Rearrangement, T-Lymphocyte ; Genes, RAG-1 ; Genetic mutation ; Genic rearrangement. Recombination. Transposable element ; Human chromosomes ; Hybridity ; Immunoglobulin Heavy Chains ; Medical research ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutation ; Nucleotides ; Receptors, Antigen, T-Cell - genetics ; Recombination, Genetic ; Reed Sternberg cells ; Transposons ; VDJ Recombinases</subject><ispartof>Science (American Association for the Advancement of Science), 1993-04, Vol.260 (5105), p.207-210</ispartof><rights>Copyright 1993 American Association for the Advancement of Science</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-3c0bf6b852db88fbb04a88d59ee636455ab3c3f9e185d1ca630c35f6e3f155d43</citedby><cites>FETCH-LOGICAL-c469t-3c0bf6b852db88fbb04a88d59ee636455ab3c3f9e185d1ca630c35f6e3f155d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2881308$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2881308$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4690928$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8469973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taccioli, Guillermo E.</creatorcontrib><creatorcontrib>Rathbun, Gary</creatorcontrib><creatorcontrib>Oltz, Eugene</creatorcontrib><creatorcontrib>Stamato, Thomas</creatorcontrib><creatorcontrib>Jeggo, Penny A.</creatorcontrib><creatorcontrib>Alt, Frederick W.</creatorcontrib><title>Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines deficient in DNA repair were tested for the ability to perform V(D)J recombination after introduction of the RAG-1 and RAG-2 genes. Two mutants failed to generate normal V(D)J recombination, and further analysis provided evidence for two distinct nonlymphoid-specific genes that encode factors involved in both DNA repair and V(D)J recombination.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>DNA Nucleotidyltransferases - genetics</subject><subject>DNA Nucleotidyltransferases - metabolism</subject><subject>DNA Repair</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Rearrangement, T-Lymphocyte</subject><subject>Genes, RAG-1</subject><subject>Genetic mutation</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>Human chromosomes</subject><subject>Hybridity</subject><subject>Immunoglobulin Heavy Chains</subject><subject>Medical research</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleotides</subject><subject>Receptors, Antigen, T-Cell - genetics</subject><subject>Recombination, Genetic</subject><subject>Reed Sternberg cells</subject><subject>Transposons</subject><subject>VDJ Recombinases</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0DtPwzAUBWALgUopzCwgZUAIhrR2HLv2CC2PoiIkXmtkO7bkksTFdgb-PakalZHpDue790oHgFMExwhldBKU1Y3SY5ZTzqd4DwwR5CTlGcT7YAghpimDU3IIjkJYQdhlHA_AoOdD8LCo18L6WjcxcSb5vJpfPyWvWrla2kZE65rENsnctbLS6Vv0oimTW6_FV4c2i8lzG0UTwzE4MKIK-qSfI_Bxf_c-e0yXLw-L2c0yVd3HmGIFpaGSkayUjBkpYS4YKwnXmmKaEyIkVthwjRgpkRIUQ4WJoRobREiZ4xG43N5de_fd6hCL2galq0o02rWhmBI6hRmC_0JE85wzsoGTLVTeheC1Kdbe1sL_FAgWm46LvuOiL63bOO9Pt7LW5c7_5Rd9LoISlelKUzbsWKcgz1jHzrZsFaLzuzhjDGHI8C8qdY37</recordid><startdate>19930409</startdate><enddate>19930409</enddate><creator>Taccioli, Guillermo E.</creator><creator>Rathbun, Gary</creator><creator>Oltz, Eugene</creator><creator>Stamato, Thomas</creator><creator>Jeggo, Penny A.</creator><creator>Alt, Frederick W.</creator><general>American Society for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930409</creationdate><title>Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants</title><author>Taccioli, Guillermo E. ; Rathbun, Gary ; Oltz, Eugene ; Stamato, Thomas ; Jeggo, Penny A. ; Alt, Frederick W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-3c0bf6b852db88fbb04a88d59ee636455ab3c3f9e185d1ca630c35f6e3f155d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>DNA Nucleotidyltransferases - genetics</topic><topic>DNA Nucleotidyltransferases - metabolism</topic><topic>DNA Repair</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Rearrangement, T-Lymphocyte</topic><topic>Genes, RAG-1</topic><topic>Genetic mutation</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>Human chromosomes</topic><topic>Hybridity</topic><topic>Immunoglobulin Heavy Chains</topic><topic>Medical research</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nucleotides</topic><topic>Receptors, Antigen, T-Cell - genetics</topic><topic>Recombination, Genetic</topic><topic>Reed Sternberg cells</topic><topic>Transposons</topic><topic>VDJ Recombinases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taccioli, Guillermo E.</creatorcontrib><creatorcontrib>Rathbun, Gary</creatorcontrib><creatorcontrib>Oltz, Eugene</creatorcontrib><creatorcontrib>Stamato, Thomas</creatorcontrib><creatorcontrib>Jeggo, Penny A.</creatorcontrib><creatorcontrib>Alt, Frederick W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taccioli, Guillermo E.</au><au>Rathbun, Gary</au><au>Oltz, Eugene</au><au>Stamato, Thomas</au><au>Jeggo, Penny A.</au><au>Alt, Frederick W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1993-04-09</date><risdate>1993</risdate><volume>260</volume><issue>5105</issue><spage>207</spage><epage>210</epage><pages>207-210</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines deficient in DNA repair were tested for the ability to perform V(D)J recombination after introduction of the RAG-1 and RAG-2 genes. Two mutants failed to generate normal V(D)J recombination, and further analysis provided evidence for two distinct nonlymphoid-specific genes that encode factors involved in both DNA repair and V(D)J recombination.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>8469973</pmid><doi>10.1126/science.8469973</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1993-04, Vol.260 (5105), p.207-210 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75670210 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Animals Base Sequence Biological and medical sciences Cell Line Cell lines CHO Cells Cricetinae DNA Nucleotidyltransferases - genetics DNA Nucleotidyltransferases - metabolism DNA Repair Fundamental and applied biological sciences. Psychology Gene Rearrangement, T-Lymphocyte Genes, RAG-1 Genetic mutation Genic rearrangement. Recombination. Transposable element Human chromosomes Hybridity Immunoglobulin Heavy Chains Medical research Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation Nucleotides Receptors, Antigen, T-Cell - genetics Recombination, Genetic Reed Sternberg cells Transposons VDJ Recombinases |
| title | Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T11%3A16%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impairment%20of%20V(D)J%20Recombination%20in%20Double-Strand%20Break%20Repair%20Mutants&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Taccioli,%20Guillermo%20E.&rft.date=1993-04-09&rft.volume=260&rft.issue=5105&rft.spage=207&rft.epage=210&rft.pages=207-210&rft.issn=0036-8075&rft.eissn=1095-9203&rft.coden=SCIEAS&rft_id=info:doi/10.1126/science.8469973&rft_dat=%3Cjstor_proqu%3E2881308%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16449850&rft_id=info:pmid/8469973&rft_jstor_id=2881308&rfr_iscdi=true |