Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene
In Arabidopsis tissues, the pool of tubulin protein is provided by the expression of multiple alpha-tubulin and beta-tubulin genes. Previous evidence suggested that the TUA2 alpha-tubulin gene was expressed in all organs of mature plants. We now report a more detailed analysis of TUA2 expression dur...
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| Veröffentlicht in: | Plant molecular biology 1993-03, Vol.21 (5), p.937-942 |
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| container_title | Plant molecular biology |
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| creator | Carpenter, J.L. (Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology) Kopczak, S.D Snustad, D.P Silflow, C.D |
| description | In Arabidopsis tissues, the pool of tubulin protein is provided by the expression of multiple alpha-tubulin and beta-tubulin genes. Previous evidence suggested that the TUA2 alpha-tubulin gene was expressed in all organs of mature plants. We now report a more detailed analysis of TUA2 expression during plant development. Chimeric genes containing TUA2 5'-flanking DNA fused to the beta-glucuronidase (GUS) coding region were used to create transgenic Arabidopsis plants. Second-generation progeny of regenerated plants were analyzed by histochemical assay to localize GUS expression. GUS activity was seen throughout plant development and in nearly all tissues. The blue product of GUS activity accumulated to the highest levels in tissues with actively dividing and elongating cells. GUS activity was not detected in a few plant tissues, suggesting that, though widely expressed, the TUA2 promoter is not constitutively active. |
| doi_str_mv | 10.1007/BF00027126 |
| format | Article |
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(Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology) ; Kopczak, S.D ; Snustad, D.P ; Silflow, C.D</creator><creatorcontrib>Carpenter, J.L. (Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology) ; Kopczak, S.D ; Snustad, D.P ; Silflow, C.D</creatorcontrib><description>In Arabidopsis tissues, the pool of tubulin protein is provided by the expression of multiple alpha-tubulin and beta-tubulin genes. Previous evidence suggested that the TUA2 alpha-tubulin gene was expressed in all organs of mature plants. We now report a more detailed analysis of TUA2 expression during plant development. Chimeric genes containing TUA2 5'-flanking DNA fused to the beta-glucuronidase (GUS) coding region were used to create transgenic Arabidopsis plants. Second-generation progeny of regenerated plants were analyzed by histochemical assay to localize GUS expression. GUS activity was seen throughout plant development and in nearly all tissues. The blue product of GUS activity accumulated to the highest levels in tissues with actively dividing and elongating cells. GUS activity was not detected in a few plant tissues, suggesting that, though widely expressed, the TUA2 promoter is not constitutively active.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00027126</identifier><identifier>PMID: 8467087</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis thaliana ; Biological and medical sciences ; Biotechnology ; CRUCIFERAE ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; GENE EXPRESSION ; Gene Expression Regulation ; GENES ; Genetic engineering ; Genetic technics ; GENETIC TRANSFORMATION ; Glucuronidase - genetics ; Methods. Procedures. Technologies ; Modification of gene expression level ; Organ Specificity ; Plant Proteins - genetics ; Plants, Genetically Modified ; Promoter Regions, Genetic ; Recombinant Fusion Proteins ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Tubulin - genetics</subject><ispartof>Plant molecular biology, 1993-03, Vol.21 (5), p.937-942</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4719684$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8467087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carpenter, J.L. (Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology)</creatorcontrib><creatorcontrib>Kopczak, S.D</creatorcontrib><creatorcontrib>Snustad, D.P</creatorcontrib><creatorcontrib>Silflow, C.D</creatorcontrib><title>Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>In Arabidopsis tissues, the pool of tubulin protein is provided by the expression of multiple alpha-tubulin and beta-tubulin genes. Previous evidence suggested that the TUA2 alpha-tubulin gene was expressed in all organs of mature plants. We now report a more detailed analysis of TUA2 expression during plant development. Chimeric genes containing TUA2 5'-flanking DNA fused to the beta-glucuronidase (GUS) coding region were used to create transgenic Arabidopsis plants. Second-generation progeny of regenerated plants were analyzed by histochemical assay to localize GUS expression. GUS activity was seen throughout plant development and in nearly all tissues. The blue product of GUS activity accumulated to the highest levels in tissues with actively dividing and elongating cells. GUS activity was not detected in a few plant tissues, suggesting that, though widely expressed, the TUA2 promoter is not constitutively active.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CRUCIFERAE</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation</subject><subject>GENES</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>GENETIC TRANSFORMATION</subject><subject>Glucuronidase - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Modification of gene expression level</subject><subject>Organ Specificity</subject><subject>Plant Proteins - genetics</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>Tubulin - genetics</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0DtLxEAUBeBBlHVdbSwFIYXYRe-8Z8p1cX2waKHW4SaZ7I7kZSYR_fcGDLZWtzgf58Al5JTCFQXQ1zdrAGCaMrVH5lRqHktgZp_MgSodC0HZITkK4R1g5FzNyMwIpcHoOXl8cZWPs6YOve-H3n-6yH21nQvBN3XUFBHW0bLD1OdNG3yI-h2WHmuMsGx3GPdDOpS-jraudsfkoMAyuJPpLsjb-vZ1dR9vnu8eVstNXDAj-zizSjKepylTGU0ly4Ri2igLRliVa-uodblBkYEGJorCYmGopI6ZVOSAli_I5W9v2zUfgwt9UvmQubLE2jVDSLRUShoJ_0I6jnJp6QjPJziklcuTtvMVdt_J9KUxv5hyDBmWRYd15sMfE5paZcTIzn5ZgU2C224kTxvLgTMu-Q_S-nxQ</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>Carpenter, J.L. (Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology)</creator><creator>Kopczak, S.D</creator><creator>Snustad, D.P</creator><creator>Silflow, C.D</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene</title><author>Carpenter, J.L. (Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology) ; Kopczak, S.D ; Snustad, D.P ; Silflow, C.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f285t-c96523dbb26c1b52c462786908496d79e19ed8a4c07024ff9af8151e28b4d0a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CRUCIFERAE</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation</topic><topic>GENES</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>GENETIC TRANSFORMATION</topic><topic>Glucuronidase - genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Modification of gene expression level</topic><topic>Organ Specificity</topic><topic>Plant Proteins - genetics</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>Tubulin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carpenter, J.L. (Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology)</creatorcontrib><creatorcontrib>Kopczak, S.D</creatorcontrib><creatorcontrib>Snustad, D.P</creatorcontrib><creatorcontrib>Silflow, C.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carpenter, J.L. (Minnesota Univ., St. Paul, MN (USA). Dept. of Genetics and Cell Biology)</au><au>Kopczak, S.D</au><au>Snustad, D.P</au><au>Silflow, C.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>21</volume><issue>5</issue><spage>937</spage><epage>942</epage><pages>937-942</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>In Arabidopsis tissues, the pool of tubulin protein is provided by the expression of multiple alpha-tubulin and beta-tubulin genes. Previous evidence suggested that the TUA2 alpha-tubulin gene was expressed in all organs of mature plants. We now report a more detailed analysis of TUA2 expression during plant development. Chimeric genes containing TUA2 5'-flanking DNA fused to the beta-glucuronidase (GUS) coding region were used to create transgenic Arabidopsis plants. Second-generation progeny of regenerated plants were analyzed by histochemical assay to localize GUS expression. GUS activity was seen throughout plant development and in nearly all tissues. The blue product of GUS activity accumulated to the highest levels in tissues with actively dividing and elongating cells. GUS activity was not detected in a few plant tissues, suggesting that, though widely expressed, the TUA2 promoter is not constitutively active.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>8467087</pmid><doi>10.1007/BF00027126</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1993-03, Vol.21 (5), p.937-942 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| subjects | Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis thaliana Biological and medical sciences Biotechnology CRUCIFERAE EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION Gene Expression Regulation GENES Genetic engineering Genetic technics GENETIC TRANSFORMATION Glucuronidase - genetics Methods. Procedures. Technologies Modification of gene expression level Organ Specificity Plant Proteins - genetics Plants, Genetically Modified Promoter Regions, Genetic Recombinant Fusion Proteins TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Tubulin - genetics |
| title | Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene |
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