The Passive Calcium Leak in Cultured Porcine Aortic Endothelial Cells
Unidirectional 45Ca fluxes were measured to characterize the calcium-leak pathway in cultured endothelial cells of pig aorta. Lanthanum, but not other calcium channel blockers such as verapamil and diltiazem, inhibited the passive 45Ca uptake. Passive uptake of 45Ca across the plasma membrane increa...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-03, Vol.191 (3), p.1197-1203 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Demirel, E. Laskey, R.E. Purkerson, S. Vanbreemen, C. |
| description | Unidirectional 45Ca fluxes were measured to characterize the calcium-leak pathway in cultured endothelial cells of pig aorta. Lanthanum, but not other calcium channel blockers such as verapamil and diltiazem, inhibited the passive 45Ca uptake. Passive uptake of 45Ca across the plasma membrane increased progressively as extracellular pH was raised from 5.2 to 9.2. SK&F 96365, a putative inhibitor of receptor-mediated Ca2+ entry, selectively blocked ATP-induced 45Ca uptake. The Ca2+ leak pathway may play an important role in endothelial cell Ca2+ homeostasis which controls the basal and stimulated release of endothelium-derived relaxing factors (i.e., EDRF, prostacyclin) in physiological or pathological situations. |
| doi_str_mv | 10.1006/bbrc.1993.1344 |
| format | Article |
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Lanthanum, but not other calcium channel blockers such as verapamil and diltiazem, inhibited the passive 45Ca uptake. Passive uptake of 45Ca across the plasma membrane increased progressively as extracellular pH was raised from 5.2 to 9.2. SK&F 96365, a putative inhibitor of receptor-mediated Ca2+ entry, selectively blocked ATP-induced 45Ca uptake. The Ca2+ leak pathway may play an important role in endothelial cell Ca2+ homeostasis which controls the basal and stimulated release of endothelium-derived relaxing factors (i.e., EDRF, prostacyclin) in physiological or pathological situations.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1344</identifier><identifier>PMID: 8466496</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adenosine Triphosphate - pharmacology ; Animals ; Aorta ; Biological and medical sciences ; Biological Transport ; Calcium - metabolism ; Calcium Channel Blockers - pharmacology ; Calcium-Transporting ATPases - metabolism ; Cell Membrane Permeability ; Cell physiology ; Cells, Cultured ; Endothelium, Vascular - metabolism ; Fundamental and applied biological sciences. 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Lanthanum, but not other calcium channel blockers such as verapamil and diltiazem, inhibited the passive 45Ca uptake. Passive uptake of 45Ca across the plasma membrane increased progressively as extracellular pH was raised from 5.2 to 9.2. SK&F 96365, a putative inhibitor of receptor-mediated Ca2+ entry, selectively blocked ATP-induced 45Ca uptake. The Ca2+ leak pathway may play an important role in endothelial cell Ca2+ homeostasis which controls the basal and stimulated release of endothelium-derived relaxing factors (i.e., EDRF, prostacyclin) in physiological or pathological situations.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Aorta</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Calcium - metabolism</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Cell Membrane Permeability</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Imidazoles - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Membrane and intracellular transports</subject><subject>Molecular and cellular biology</subject><subject>Nitric Oxide - metabolism</subject><subject>Swine</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EKqWwsiF5QGwpdux8jSgqH1IlOhSJzXLOF9WQj2Inlfj3JGrUjemG97lXdw8ht5wtOWPxY1E4WPIsE0supDwjc84yFoScyXMyZwMRhBn_vCRX3n8xxrmMsxmZpTKOZRbPyWq7Q7rR3tsD0lxXYPuarlF_U9vQvK-63qGhm9aBbZA-ta6zQFeNabsdVlZXNMeq8tfkotSVx5tpLsjH82qbvwbr95e3_GkdgGRpFxRliMKERQyFBp5oWWgTQiST0JSZAJ0YLWQIEkVkIkxFmaAs4yzWMjKQcBAL8nDs3bv2p0ffqdp6GC7QDba9V0k0vMVCMYDLIwiu9d5hqfbO1tr9Ks7U6E2N3tToTY3ehoW7qbkvajQnfBI15PdTrj3oqnS6AetPmEwk4ywZsPSI4WDhYNEpDxYbQGMdQqdMa_-74A-S_IkH</recordid><startdate>19930331</startdate><enddate>19930331</enddate><creator>Demirel, E.</creator><creator>Laskey, R.E.</creator><creator>Purkerson, S.</creator><creator>Vanbreemen, C.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930331</creationdate><title>The Passive Calcium Leak in Cultured Porcine Aortic Endothelial Cells</title><author>Demirel, E. ; Laskey, R.E. ; Purkerson, S. ; Vanbreemen, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-bf2e3d2b6cbac17a4bad2c5472df93ca7da342c4e35d5e83f7e4f696a45dc71c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Aorta</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Calcium - metabolism</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Cell Membrane Permeability</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Imidazoles - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Membrane and intracellular transports</topic><topic>Molecular and cellular biology</topic><topic>Nitric Oxide - metabolism</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demirel, E.</creatorcontrib><creatorcontrib>Laskey, R.E.</creatorcontrib><creatorcontrib>Purkerson, S.</creatorcontrib><creatorcontrib>Vanbreemen, C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demirel, E.</au><au>Laskey, R.E.</au><au>Purkerson, S.</au><au>Vanbreemen, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Passive Calcium Leak in Cultured Porcine Aortic Endothelial Cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-03-31</date><risdate>1993</risdate><volume>191</volume><issue>3</issue><spage>1197</spage><epage>1203</epage><pages>1197-1203</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Unidirectional 45Ca fluxes were measured to characterize the calcium-leak pathway in cultured endothelial cells of pig aorta. Lanthanum, but not other calcium channel blockers such as verapamil and diltiazem, inhibited the passive 45Ca uptake. Passive uptake of 45Ca across the plasma membrane increased progressively as extracellular pH was raised from 5.2 to 9.2. SK&F 96365, a putative inhibitor of receptor-mediated Ca2+ entry, selectively blocked ATP-induced 45Ca uptake. The Ca2+ leak pathway may play an important role in endothelial cell Ca2+ homeostasis which controls the basal and stimulated release of endothelium-derived relaxing factors (i.e., EDRF, prostacyclin) in physiological or pathological situations.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8466496</pmid><doi>10.1006/bbrc.1993.1344</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Triphosphate - pharmacology Animals Aorta Biological and medical sciences Biological Transport Calcium - metabolism Calcium Channel Blockers - pharmacology Calcium-Transporting ATPases - metabolism Cell Membrane Permeability Cell physiology Cells, Cultured Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Imidazoles - pharmacology In Vitro Techniques Membrane and intracellular transports Molecular and cellular biology Nitric Oxide - metabolism Swine |
| title | The Passive Calcium Leak in Cultured Porcine Aortic Endothelial Cells |
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