Detection of "Clostridium difficile" on minimal and selective medium and by immunofluorescence antibody staining (author's transl)

A minimal and selective medium containing 5 aminoacids, 3 vitamins, a low sugar concentration and 2 antibiotics (cefoxitin at 16 microgram/ml and streptomycin at 500 microgram/ml) is described for isolation of Clostridium difficile from the gut. Comparable results were obtained with this medium and...

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Veröffentlicht in:	Annales de microbiologie 1981-03, Vol.132 (2), p.149-157
Hauptverfasser:	Hubert, J, Ionesco, H, Sebald, M
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Sprache:	fre
Schlagworte:	Animals Anti-Bacterial Agents - therapeutic use Clostridium - immunology Clostridium - isolation & purification Cricetinae Culture Media Enterocolitis, Pseudomembranous - drug therapy Enterocolitis, Pseudomembranous - microbiology Fluorescent Antibody Technique Humans
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container_title	Annales de microbiologie
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creator	Hubert, J Ionesco, H Sebald, M
description	A minimal and selective medium containing 5 aminoacids, 3 vitamins, a low sugar concentration and 2 antibiotics (cefoxitin at 16 microgram/ml and streptomycin at 500 microgram/ml) is described for isolation of Clostridium difficile from the gut. Comparable results were obtained with this medium and using spore isolation by the sodium thioglycolate-lysozyme technique. An antiserum specific to C. difficile was prepared and used for detection by immunofluorescent antibody staining. This is a very sensitive technique which leads to a quick and early identification of this bacterial species.
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subjects	Animals Anti-Bacterial Agents - therapeutic use Clostridium - immunology Clostridium - isolation & purification Cricetinae Culture Media Enterocolitis, Pseudomembranous - drug therapy Enterocolitis, Pseudomembranous - microbiology Fluorescent Antibody Technique Humans
title	Detection of "Clostridium difficile" on minimal and selective medium and by immunofluorescence antibody staining (author's transl)
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