Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells
A study was undertaken to evaluate embryonic development and establish pregnancies with human embryos after in-vitro culture in two different systems. Treatment A consisted of culturing zygotes in serum-supplemented human tubal fluid culture medium (HTF). Treatment B consisted of culturing zygotes o...
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| Veröffentlicht in: | Human reproduction (Oxford) 1993-01, Vol.8 (1), p.97-101 |
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| creator | Wiemer, K.E. Hoffman, D.I. Maxson, W.S. Eager, S. Muhlberger, B. Fiore, I. Cuervo, M. |
| description | A study was undertaken to evaluate embryonic development and establish pregnancies with human embryos after in-vitro culture in two different systems. Treatment A consisted of culturing zygotes in serum-supplemented human tubal fluid culture medium (HTF). Treatment B consisted of culturing zygotes on a monolayer of bovine oviductal epithelial cells with HTF. At the time of embryo replacement, embryos in treatment B had 4.11 blastomeres present, which was greater (P < 0.05) than the 3.81 present for embryos in treatment A. In addition, the cellular fragmentation rate for treatment A embryos was 1.10, which was greater (P < 0.05) than the fragmentation rate of 0.38 for embryos within treatment B. The incidence of ongoing pregnancy was higher after replacement of co-cultured embryos (treatment B) (43%) than replacement of conventionally cultured embryos (treatment A) (29%). The implantation rate per embryo increased (P < 0.05) from 11.5 to 18.4% after co-culture. In treatment B the proportion of ‘spare’ embryos developing to expanded blastocysts was 58.5%, which was greater (P < 0.05) than the blastocyst development rate of 29.3% observed for embryos within treatment A. |
| doi_str_mv | 10.1093/oxfordjournals.humrep.a137884 |
| format | Article |
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Treatment A consisted of culturing zygotes in serum-supplemented human tubal fluid culture medium (HTF). Treatment B consisted of culturing zygotes on a monolayer of bovine oviductal epithelial cells with HTF. At the time of embryo replacement, embryos in treatment B had 4.11 blastomeres present, which was greater (P < 0.05) than the 3.81 present for embryos in treatment A. In addition, the cellular fragmentation rate for treatment A embryos was 1.10, which was greater (P < 0.05) than the fragmentation rate of 0.38 for embryos within treatment B. The incidence of ongoing pregnancy was higher after replacement of co-cultured embryos (treatment B) (43%) than replacement of conventionally cultured embryos (treatment A) (29%). The implantation rate per embryo increased (P < 0.05) from 11.5 to 18.4% after co-culture. In treatment B the proportion of ‘spare’ embryos developing to expanded blastocysts was 58.5%, which was greater (P < 0.05) than the blastocyst development rate of 29.3% observed for embryos within treatment A.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/oxfordjournals.humrep.a137884</identifier><identifier>PMID: 8458937</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Biological and medical sciences ; Birth control ; Cattle ; Cells, Cultured ; co-culture ; embryo development ; Embryo Implantation - physiology ; Embryo, Mammalian - physiology ; Embryonic and Fetal Development - physiology ; Epithelial Cells ; Fallopian Tubes - cytology ; Female ; Gynecology. Andrology. Obstetrics ; human implantation ; Humans ; Male ; Medical sciences ; Sterility. Assisted procreation ; Zygote - physiology</subject><ispartof>Human reproduction (Oxford), 1993-01, Vol.8 (1), p.97-101</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c302t-53c6d9aa9e23c7972c968c3a9f97884616ac4e1557e9fdf2d112a1fa597f5d243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4632766$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8458937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wiemer, K.E.</creatorcontrib><creatorcontrib>Hoffman, D.I.</creatorcontrib><creatorcontrib>Maxson, W.S.</creatorcontrib><creatorcontrib>Eager, S.</creatorcontrib><creatorcontrib>Muhlberger, B.</creatorcontrib><creatorcontrib>Fiore, I.</creatorcontrib><creatorcontrib>Cuervo, M.</creatorcontrib><title>Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>A study was undertaken to evaluate embryonic development and establish pregnancies with human embryos after in-vitro culture in two different systems. Treatment A consisted of culturing zygotes in serum-supplemented human tubal fluid culture medium (HTF). Treatment B consisted of culturing zygotes on a monolayer of bovine oviductal epithelial cells with HTF. At the time of embryo replacement, embryos in treatment B had 4.11 blastomeres present, which was greater (P < 0.05) than the 3.81 present for embryos in treatment A. In addition, the cellular fragmentation rate for treatment A embryos was 1.10, which was greater (P < 0.05) than the fragmentation rate of 0.38 for embryos within treatment B. The incidence of ongoing pregnancy was higher after replacement of co-cultured embryos (treatment B) (43%) than replacement of conventionally cultured embryos (treatment A) (29%). The implantation rate per embryo increased (P < 0.05) from 11.5 to 18.4% after co-culture. In treatment B the proportion of ‘spare’ embryos developing to expanded blastocysts was 58.5%, which was greater (P < 0.05) than the blastocyst development rate of 29.3% observed for embryos within treatment A.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>co-culture</subject><subject>embryo development</subject><subject>Embryo Implantation - physiology</subject><subject>Embryo, Mammalian - physiology</subject><subject>Embryonic and Fetal Development - physiology</subject><subject>Epithelial Cells</subject><subject>Fallopian Tubes - cytology</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>human implantation</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Sterility. Assisted procreation</subject><subject>Zygote - physiology</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkFGP1CAUhYnRrOPqTzDhQfetYykFyoMPZrO6JpOYmDGZ-ELuUNhhpVChXWf-va3TTOITh5xzuJcPofekXJNS0g_xaGNqH-OYAvi8PoxdMv0aCBVNUz9DK1LzsqgoK5-jVVnxpiCEk5foVc6PZTnJhl-hq6ZmjaRihY533T6dYnAadzH1h-jjwwlDaHGCweBoset6D2GAwcUw36eBELD5V8vYRu_jHxcesI6FHv0wpqkV8D4-uTCpJ9eOegCPTe-Gg_Fuktp4n1-jF3ba37xZzmv04_Pd9va-2Hz78vX206bQtKyGglHNWwkgTUW1kKLSkjeagrRy_i8nHHRtCGPCSNvaqiWkAmKBSWFZW9X0Gt2c3-1T_D2aPKjO5XkDCCaOWQnGmSR8Dn48B3WKOSdjVZ9cB-mkSKlm8up_8upMXi3kp_7bZdC470x7aS-oJ__d4kPW4G2CoF2-xGpOK8H5FCvOMZcHc7zYkH4pLqhg6n73U-22my3fbHfqO_0LxUenLw</recordid><startdate>199301</startdate><enddate>199301</enddate><creator>Wiemer, K.E.</creator><creator>Hoffman, D.I.</creator><creator>Maxson, W.S.</creator><creator>Eager, S.</creator><creator>Muhlberger, B.</creator><creator>Fiore, I.</creator><creator>Cuervo, M.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199301</creationdate><title>Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells</title><author>Wiemer, K.E. ; Hoffman, D.I. ; Maxson, W.S. ; Eager, S. ; Muhlberger, B. ; Fiore, I. ; Cuervo, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-53c6d9aa9e23c7972c968c3a9f97884616ac4e1557e9fdf2d112a1fa597f5d243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>co-culture</topic><topic>embryo development</topic><topic>Embryo Implantation - physiology</topic><topic>Embryo, Mammalian - physiology</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Epithelial Cells</topic><topic>Fallopian Tubes - cytology</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>human implantation</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Sterility. Assisted procreation</topic><topic>Zygote - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wiemer, K.E.</creatorcontrib><creatorcontrib>Hoffman, D.I.</creatorcontrib><creatorcontrib>Maxson, W.S.</creatorcontrib><creatorcontrib>Eager, S.</creatorcontrib><creatorcontrib>Muhlberger, B.</creatorcontrib><creatorcontrib>Fiore, I.</creatorcontrib><creatorcontrib>Cuervo, M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wiemer, K.E.</au><au>Hoffman, D.I.</au><au>Maxson, W.S.</au><au>Eager, S.</au><au>Muhlberger, B.</au><au>Fiore, I.</au><au>Cuervo, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>1993-01</date><risdate>1993</risdate><volume>8</volume><issue>1</issue><spage>97</spage><epage>101</epage><pages>97-101</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>A study was undertaken to evaluate embryonic development and establish pregnancies with human embryos after in-vitro culture in two different systems. Treatment A consisted of culturing zygotes in serum-supplemented human tubal fluid culture medium (HTF). Treatment B consisted of culturing zygotes on a monolayer of bovine oviductal epithelial cells with HTF. At the time of embryo replacement, embryos in treatment B had 4.11 blastomeres present, which was greater (P < 0.05) than the 3.81 present for embryos in treatment A. In addition, the cellular fragmentation rate for treatment A embryos was 1.10, which was greater (P < 0.05) than the fragmentation rate of 0.38 for embryos within treatment B. The incidence of ongoing pregnancy was higher after replacement of co-cultured embryos (treatment B) (43%) than replacement of conventionally cultured embryos (treatment A) (29%). The implantation rate per embryo increased (P < 0.05) from 11.5 to 18.4% after co-culture. In treatment B the proportion of ‘spare’ embryos developing to expanded blastocysts was 58.5%, which was greater (P < 0.05) than the blastocyst development rate of 29.3% observed for embryos within treatment A.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>8458937</pmid><doi>10.1093/oxfordjournals.humrep.a137884</doi><tpages>5</tpages></addata></record> |
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| ispartof | Human reproduction (Oxford), 1993-01, Vol.8 (1), p.97-101 |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE |
| subjects | Animals Biological and medical sciences Birth control Cattle Cells, Cultured co-culture embryo development Embryo Implantation - physiology Embryo, Mammalian - physiology Embryonic and Fetal Development - physiology Epithelial Cells Fallopian Tubes - cytology Female Gynecology. Andrology. Obstetrics human implantation Humans Male Medical sciences Sterility. Assisted procreation Zygote - physiology |
| title | Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells |
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