Gel electrophoresis of giant proteins: Solubilization and silver-staining of titin and nebulin from single muscle fiber segments

Giant proteins in the megadalton range (> 0.5 MDa) appear to play important structural and functional roles in striated muscle. Titin (∼ 3 MDa) is involved in the generation of resting tension and the assembly and stability of the sarcomere in skeletal and cardiac muscle tissues, while nebulin (∼...

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