Rapid production of a panel of blood group A-active oligosaccharides using chemically synthesized di- and tri-saccharide primers and an easily prepared porcine (1 → 3)-α- N-acetyl- d-galactosaminyltransferase
A porcine (1 → 3)-α- N-acetyl- d-galactosaminyltransferse was obtained in a state suitable for preparative-scale (mg-scale) synthesis using simple procedures requiring only three days of effort. The enzyme thus prepared transferred GalNAc efficiently from UDP-GalNAc to six different chemically synth...
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| Veröffentlicht in: | Carbohydrate research 1993-02, Vol.239, p.167-176 |
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| container_title | Carbohydrate research |
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| creator | Compston, Catharine A. Condon, Caro Hanna, H.Rizk Mazid, M.Abdul |
| description | A porcine (1 → 3)-α-
N-acetyl-
d-galactosaminyltransferse was obtained in a state suitable for preparative-scale (mg-scale) synthesis using simple procedures requiring only three days of effort. The enzyme thus prepared transferred GalNAc efficiently from UDP-GalNAc to six different chemically synthesized di- and tri-saccharide H-active structures to yield blood-group A-active oligosaccharides that were characterized by
1H NMR spectroscopy and mass spectrometry. This work further demonstrates the efficiency and attractiveness of using glycosyltransferases in a combined chemoenzymatic approach for the rapid production of biologically active oligosaccharides. |
| doi_str_mv | 10.1016/0008-6215(93)84212-O |
| format | Article |
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d-galactosaminyltransferse was obtained in a state suitable for preparative-scale (mg-scale) synthesis using simple procedures requiring only three days of effort. The enzyme thus prepared transferred GalNAc efficiently from UDP-GalNAc to six different chemically synthesized di- and tri-saccharide H-active structures to yield blood-group A-active oligosaccharides that were characterized by
1H NMR spectroscopy and mass spectrometry. This work further demonstrates the efficiency and attractiveness of using glycosyltransferases in a combined chemoenzymatic approach for the rapid production of biologically active oligosaccharides.</description><subject>ABO Blood-Group System - chemistry</subject><subject>Animals</subject><subject>Carbohydrate Sequence</subject><subject>Disaccharides - chemistry</subject><subject>Gastric Mucosa - enzymology</subject><subject>Glycosylation</subject><subject>Molecular Sequence Data</subject><subject>N-Acetylgalactosaminyltransferases - isolation & purification</subject><subject>N-Acetylgalactosaminyltransferases - metabolism</subject><subject>Oligosaccharides - chemistry</subject><subject>Swine</subject><subject>Trisaccharides - chemistry</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcuKFDEUDaKM7egfKGQlM4topR6pykYYBl8w2CAK7sLt5FZ3JJ2USdVA-QF-gH_ij7jxD_wSU9PNuNNVbjjn3Hs4h5DHvHjGCy6eF0XRMVHy5kxW511d8pKt75AV79qK1aX4dJesbin3yYOUPudvIVpxQk66ummlrFfk13sYrKFDDGbSow2ehp4CHcCjW8aNC8HQbQzTQC8YZMo10uDsNiTQegfRGkx0StZvqd7h3mpwbqZp9uMOk_2KhhrLKHhDx2jZX1E-afcY0w0EniIkm4VDxAFiVg0hauuRnnH6-9t3Wp2znz8YfZct4Dg7Rg3bgst-so-99bMbI_jUY4SED8m9HlzCR8f3lHx89fLD5Rt2tX799vLiiumqqEaGgmMlBBpZS9H1nRS81xvetthIg4BCgyxzqtwY3fQIXc8LbASvhdASNl11Sp4e9ub0vkyYRrW3SaNzObwwJdU2omnK7v9ELupsoeKZWB-IOoaUIvZqSQnirHihltLV0qhaGlWyUjelq3WWPTnunzZ7NLeiY8sZf3HAMadxbTGqpC16jcZG1KMywf77wB-oasFg</recordid><startdate>19930201</startdate><enddate>19930201</enddate><creator>Compston, Catharine A.</creator><creator>Condon, Caro</creator><creator>Hanna, H.Rizk</creator><creator>Mazid, M.Abdul</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19930201</creationdate><title>Rapid production of a panel of blood group A-active oligosaccharides using chemically synthesized di- and tri-saccharide primers and an easily prepared porcine (1 → 3)-α- N-acetyl- d-galactosaminyltransferase</title><author>Compston, Catharine A. ; Condon, Caro ; Hanna, H.Rizk ; Mazid, M.Abdul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-e61e366ed94968f8961fcb177e59deae6ca922121ddc5fea8f10e561466c9ab83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ABO Blood-Group System - chemistry</topic><topic>Animals</topic><topic>Carbohydrate Sequence</topic><topic>Disaccharides - chemistry</topic><topic>Gastric Mucosa - enzymology</topic><topic>Glycosylation</topic><topic>Molecular Sequence Data</topic><topic>N-Acetylgalactosaminyltransferases - isolation & purification</topic><topic>N-Acetylgalactosaminyltransferases - metabolism</topic><topic>Oligosaccharides - chemistry</topic><topic>Swine</topic><topic>Trisaccharides - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Compston, Catharine A.</creatorcontrib><creatorcontrib>Condon, Caro</creatorcontrib><creatorcontrib>Hanna, H.Rizk</creatorcontrib><creatorcontrib>Mazid, M.Abdul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Compston, Catharine A.</au><au>Condon, Caro</au><au>Hanna, H.Rizk</au><au>Mazid, M.Abdul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid production of a panel of blood group A-active oligosaccharides using chemically synthesized di- and tri-saccharide primers and an easily prepared porcine (1 → 3)-α- N-acetyl- d-galactosaminyltransferase</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>1993-02-01</date><risdate>1993</risdate><volume>239</volume><spage>167</spage><epage>176</epage><pages>167-176</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>A porcine (1 → 3)-α-
N-acetyl-
d-galactosaminyltransferse was obtained in a state suitable for preparative-scale (mg-scale) synthesis using simple procedures requiring only three days of effort. The enzyme thus prepared transferred GalNAc efficiently from UDP-GalNAc to six different chemically synthesized di- and tri-saccharide H-active structures to yield blood-group A-active oligosaccharides that were characterized by
1H NMR spectroscopy and mass spectrometry. This work further demonstrates the efficiency and attractiveness of using glycosyltransferases in a combined chemoenzymatic approach for the rapid production of biologically active oligosaccharides.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>8457994</pmid><doi>10.1016/0008-6215(93)84212-O</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-6215 |
| ispartof | Carbohydrate research, 1993-02, Vol.239, p.167-176 |
| issn | 0008-6215 1873-426X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75655288 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | ABO Blood-Group System - chemistry Animals Carbohydrate Sequence Disaccharides - chemistry Gastric Mucosa - enzymology Glycosylation Molecular Sequence Data N-Acetylgalactosaminyltransferases - isolation & purification N-Acetylgalactosaminyltransferases - metabolism Oligosaccharides - chemistry Swine Trisaccharides - chemistry |
| title | Rapid production of a panel of blood group A-active oligosaccharides using chemically synthesized di- and tri-saccharide primers and an easily prepared porcine (1 → 3)-α- N-acetyl- d-galactosaminyltransferase |
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