Primary structure of N‐linked carbohydrate chains of a human chimeric plasminogen activator K2tu‐PA expressed in Chinese hamster ovary cells

A recombinant human plasminogen activator hybrid variant K2tu‐PA, expressed in Chinese hamster ovary cells, is partially glycosylated at Asn12 (A chain, kringle‐2 domain) and completely glycosylated at Asn247 (B chain, protease domain). After release of the N‐linked carbohydrate chains by peptide‐N4...

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Veröffentlicht in:	European journal of biochemistry 1993-03, Vol.212 (3), p.639-656
Hauptverfasser:	BERGWERFF, Aldert A., OOSTRUM, Jan, ASSELBERGS, Fred A. M., BÜRGI, Rolf, HOKKE, Cornelis H., KAMERLING, Johannis P., VLIEGENTHART, Johannes F. G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Asparagine Biological and medical sciences Carbohydrate Conformation Carbohydrate Sequence Chimera CHO Cells Cricetinae Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genetic Variation Glycopeptides - isolation & purification Glycosylation Humans Hydrolases Molecular Sequence Data Oligosaccharides - chemistry Oligosaccharides - isolation & purification Peptide Mapping Protein Structure, Secondary Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Tissue Plasminogen Activator - biosynthesis Tissue Plasminogen Activator - chemistry Tissue Plasminogen Activator - genetics Transfection Urokinase-Type Plasminogen Activator - biosynthesis Urokinase-Type Plasminogen Activator - chemistry Urokinase-Type Plasminogen Activator - genetics
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