Molecular characterization of a dense granule antigen (Gra 2) associated with the network of the parasitophorous vacuole in Toxoplasma gondii
The monoclonal antibody (mAb) TG17.179 recognizes an excreted-secreted antigen (ESA) of 28.5 kDa named Gra 2, which is stored in the dense granules of Toxoplasma cells and secreted into the parasitophorous vacuole after host cell invasion. Screening of an expression cDNA library with TG17.179 led to...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1993-03, Vol.58 (1), p.71-82 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Mercier, Corinne Lecordier, Laurence Darcy, Françoise Deslee, Didier Murray, Avril Tourvieille, Béatrice Maes, Pierrette Capron, André Cesbron-Delauw, Marie-France |
| description | The monoclonal antibody (mAb) TG17.179 recognizes an excreted-secreted antigen (ESA) of 28.5 kDa named Gra 2, which is stored in the dense granules of
Toxoplasma cells and secreted into the parasitophorous vacuole after host cell invasion. Screening of an expression cDNA library with TG17.179 led to the isolation of several clones, the longest one (clone L) being of 1030 bp. Clone L cDNA was found to be homologous to a previously described composite cDNA encoding a P28 protein of
Toxoplasma gondii. Characterization of one genomic clone indicates that the complete
GRA 2 gene is about 1.3 kb in length, including an intron of 241 bp. Northern blot and primer extension analyses confirmed the size of the mature messenger (1.1 kb). Amino acid partial sequencing of the native antigen purified by HPLC and metabolic radiolabelings of ESAs perfectly matched the primary amino acid structure deduced from the clone L cDNA. This primary translation product consists of an 185 amino acid polypeptide (19.8 kDa) including a 23 amino acid signal sequence. The presence of many serine and threonine residues may indicate an
O-glycosylation. The predicted mature polypeptide shows an internal helical domain with 2 amphipathic α-helices. These might be involved in the association of Gra 2 with the membranous network within the parasitophorous vacuole. |
| doi_str_mv | 10.1016/0166-6851(93)90092-C |
| format | Article |
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Toxoplasma cells and secreted into the parasitophorous vacuole after host cell invasion. Screening of an expression cDNA library with TG17.179 led to the isolation of several clones, the longest one (clone L) being of 1030 bp. Clone L cDNA was found to be homologous to a previously described composite cDNA encoding a P28 protein of
Toxoplasma gondii. Characterization of one genomic clone indicates that the complete
GRA 2 gene is about 1.3 kb in length, including an intron of 241 bp. Northern blot and primer extension analyses confirmed the size of the mature messenger (1.1 kb). Amino acid partial sequencing of the native antigen purified by HPLC and metabolic radiolabelings of ESAs perfectly matched the primary amino acid structure deduced from the clone L cDNA. This primary translation product consists of an 185 amino acid polypeptide (19.8 kDa) including a 23 amino acid signal sequence. The presence of many serine and threonine residues may indicate an
O-glycosylation. The predicted mature polypeptide shows an internal helical domain with 2 amphipathic α-helices. These might be involved in the association of Gra 2 with the membranous network within the parasitophorous vacuole.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(93)90092-C</identifier><identifier>PMID: 8384696</identifier><identifier>CODEN: MBIPDP</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Protozoan - immunology ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Antigens, Protozoan - isolation & purification ; Base Sequence ; Biological and medical sciences ; Dense granule ; Excreted-secreted antigen ; Exons - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Gene structure ; Genes, Protozoan - genetics ; Genes. Genome ; Inclusion Bodies - chemistry ; Inclusion Bodies - immunology ; Introns - genetics ; Invertebrates ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Protein Structure, Secondary ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Protozoan Proteins - isolation & purification ; Sequence Analysis, DNA ; Toxoplasma - genetics ; Toxoplasma - immunology ; Toxoplasma - ultrastructure ; Toxoplasma gondii ; Tropical medicine ; Vacuoles - chemistry ; Vacuoles - immunology</subject><ispartof>Molecular and biochemical parasitology, 1993-03, Vol.58 (1), p.71-82</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-8bda165b5b01c09d2d8271b197afadf28247425ee0a4ad3728523b863b4f719d3</citedby><cites>FETCH-LOGICAL-c386t-8bda165b5b01c09d2d8271b197afadf28247425ee0a4ad3728523b863b4f719d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-6851(93)90092-C$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4697941$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8384696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mercier, Corinne</creatorcontrib><creatorcontrib>Lecordier, Laurence</creatorcontrib><creatorcontrib>Darcy, Françoise</creatorcontrib><creatorcontrib>Deslee, Didier</creatorcontrib><creatorcontrib>Murray, Avril</creatorcontrib><creatorcontrib>Tourvieille, Béatrice</creatorcontrib><creatorcontrib>Maes, Pierrette</creatorcontrib><creatorcontrib>Capron, André</creatorcontrib><creatorcontrib>Cesbron-Delauw, Marie-France</creatorcontrib><title>Molecular characterization of a dense granule antigen (Gra 2) associated with the network of the parasitophorous vacuole in Toxoplasma gondii</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>The monoclonal antibody (mAb) TG17.179 recognizes an excreted-secreted antigen (ESA) of 28.5 kDa named Gra 2, which is stored in the dense granules of
Toxoplasma cells and secreted into the parasitophorous vacuole after host cell invasion. Screening of an expression cDNA library with TG17.179 led to the isolation of several clones, the longest one (clone L) being of 1030 bp. Clone L cDNA was found to be homologous to a previously described composite cDNA encoding a P28 protein of
Toxoplasma gondii. Characterization of one genomic clone indicates that the complete
GRA 2 gene is about 1.3 kb in length, including an intron of 241 bp. Northern blot and primer extension analyses confirmed the size of the mature messenger (1.1 kb). Amino acid partial sequencing of the native antigen purified by HPLC and metabolic radiolabelings of ESAs perfectly matched the primary amino acid structure deduced from the clone L cDNA. This primary translation product consists of an 185 amino acid polypeptide (19.8 kDa) including a 23 amino acid signal sequence. The presence of many serine and threonine residues may indicate an
O-glycosylation. The predicted mature polypeptide shows an internal helical domain with 2 amphipathic α-helices. These might be involved in the association of Gra 2 with the membranous network within the parasitophorous vacuole.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Protozoan - immunology</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Antigens, Protozoan - isolation & purification</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Dense granule</subject><subject>Excreted-secreted antigen</subject><subject>Exons - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Gene structure</subject><subject>Genes, Protozoan - genetics</subject><subject>Genes. Genome</subject><subject>Inclusion Bodies - chemistry</subject><subject>Inclusion Bodies - immunology</subject><subject>Introns - genetics</subject><subject>Invertebrates</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Protein Structure, Secondary</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Protozoan Proteins - isolation & purification</subject><subject>Sequence Analysis, DNA</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma - ultrastructure</subject><subject>Toxoplasma gondii</subject><subject>Tropical medicine</subject><subject>Vacuoles - chemistry</subject><subject>Vacuoles - immunology</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhiMEKtvCG4DkA0LtIWA7iWNfkNAKClIRl3K2JvZk15C1F9tpgXfgneuwqz1ysEbWfP8_o3-q6gWjbxhl4m15ohayY5equVKUKl6vH1UrJnteq5bLx9XqhDytzlP6TinteiHOqjPZyFYosar-fgkTmnmCSMwWIpiM0f2B7IInYSRALPqEZBPBzxMS8Nlt0JPL6wiEXxFIKRgHGS25d3lL8haJx3wf4o9Fvnz3xTW5HPbbEMOcyB2YucwkzpPb8CvsJ0g7IJvgrXPPqicjTAmfH-tF9e3jh9v1p_rm6_Xn9fub2jRS5FoOFpjohm6gzFBluZW8ZwNTPYxgRy5527e8Q6TQgm16LjveDFI0Qzv2TNnmonp98N3H8HPGlPXOJYPTBB7LjrrvRCspUwVsD6CJIaWIo95Ht4P4WzOqlyvoJWK9RKxVo_9dQa-L7OXRfx52aE-iY-yl_-rYh2RgGku6xqUTVphetaxg7w4YlizuHEadjENv0LqIJmsb3P_3eABmeqWR</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>Mercier, Corinne</creator><creator>Lecordier, Laurence</creator><creator>Darcy, Françoise</creator><creator>Deslee, Didier</creator><creator>Murray, Avril</creator><creator>Tourvieille, Béatrice</creator><creator>Maes, Pierrette</creator><creator>Capron, André</creator><creator>Cesbron-Delauw, Marie-France</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>Molecular characterization of a dense granule antigen (Gra 2) associated with the network of the parasitophorous vacuole in Toxoplasma gondii</title><author>Mercier, Corinne ; Lecordier, Laurence ; Darcy, Françoise ; Deslee, Didier ; Murray, Avril ; Tourvieille, Béatrice ; Maes, Pierrette ; Capron, André ; Cesbron-Delauw, Marie-France</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-8bda165b5b01c09d2d8271b197afadf28247425ee0a4ad3728523b863b4f719d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Protozoan - immunology</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Antigens, Protozoan - isolation & purification</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Dense granule</topic><topic>Excreted-secreted antigen</topic><topic>Exons - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Gene structure</topic><topic>Genes, Protozoan - genetics</topic><topic>Genes. Genome</topic><topic>Inclusion Bodies - chemistry</topic><topic>Inclusion Bodies - immunology</topic><topic>Introns - genetics</topic><topic>Invertebrates</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Protein Structure, Secondary</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Protozoan Proteins - isolation & purification</topic><topic>Sequence Analysis, DNA</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma - ultrastructure</topic><topic>Toxoplasma gondii</topic><topic>Tropical medicine</topic><topic>Vacuoles - chemistry</topic><topic>Vacuoles - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mercier, Corinne</creatorcontrib><creatorcontrib>Lecordier, Laurence</creatorcontrib><creatorcontrib>Darcy, Françoise</creatorcontrib><creatorcontrib>Deslee, Didier</creatorcontrib><creatorcontrib>Murray, Avril</creatorcontrib><creatorcontrib>Tourvieille, Béatrice</creatorcontrib><creatorcontrib>Maes, Pierrette</creatorcontrib><creatorcontrib>Capron, André</creatorcontrib><creatorcontrib>Cesbron-Delauw, Marie-France</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mercier, Corinne</au><au>Lecordier, Laurence</au><au>Darcy, Françoise</au><au>Deslee, Didier</au><au>Murray, Avril</au><au>Tourvieille, Béatrice</au><au>Maes, Pierrette</au><au>Capron, André</au><au>Cesbron-Delauw, Marie-France</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of a dense granule antigen (Gra 2) associated with the network of the parasitophorous vacuole in Toxoplasma gondii</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>58</volume><issue>1</issue><spage>71</spage><epage>82</epage><pages>71-82</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><coden>MBIPDP</coden><abstract>The monoclonal antibody (mAb) TG17.179 recognizes an excreted-secreted antigen (ESA) of 28.5 kDa named Gra 2, which is stored in the dense granules of
Toxoplasma cells and secreted into the parasitophorous vacuole after host cell invasion. Screening of an expression cDNA library with TG17.179 led to the isolation of several clones, the longest one (clone L) being of 1030 bp. Clone L cDNA was found to be homologous to a previously described composite cDNA encoding a P28 protein of
Toxoplasma gondii. Characterization of one genomic clone indicates that the complete
GRA 2 gene is about 1.3 kb in length, including an intron of 241 bp. Northern blot and primer extension analyses confirmed the size of the mature messenger (1.1 kb). Amino acid partial sequencing of the native antigen purified by HPLC and metabolic radiolabelings of ESAs perfectly matched the primary amino acid structure deduced from the clone L cDNA. This primary translation product consists of an 185 amino acid polypeptide (19.8 kDa) including a 23 amino acid signal sequence. The presence of many serine and threonine residues may indicate an
O-glycosylation. The predicted mature polypeptide shows an internal helical domain with 2 amphipathic α-helices. These might be involved in the association of Gra 2 with the membranous network within the parasitophorous vacuole.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>8384696</pmid><doi>10.1016/0166-6851(93)90092-C</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular and biochemical parasitology, 1993-03, Vol.58 (1), p.71-82 |
| issn | 0166-6851 1872-9428 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Animals Antibodies, Protozoan - immunology Antigens, Protozoan - genetics Antigens, Protozoan - immunology Antigens, Protozoan - isolation & purification Base Sequence Biological and medical sciences Dense granule Excreted-secreted antigen Exons - genetics Fundamental and applied biological sciences. Psychology Gene Library Gene structure Genes, Protozoan - genetics Genes. Genome Inclusion Bodies - chemistry Inclusion Bodies - immunology Introns - genetics Invertebrates Molecular and cellular biology Molecular genetics Molecular Sequence Data Protein Structure, Secondary Protozoan Proteins - genetics Protozoan Proteins - immunology Protozoan Proteins - isolation & purification Sequence Analysis, DNA Toxoplasma - genetics Toxoplasma - immunology Toxoplasma - ultrastructure Toxoplasma gondii Tropical medicine Vacuoles - chemistry Vacuoles - immunology |
| title | Molecular characterization of a dense granule antigen (Gra 2) associated with the network of the parasitophorous vacuole in Toxoplasma gondii |
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