Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein
Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus in...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1993-03, Vol.259 (5102), p.1745-1749 |
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| creator | Ulmer, Jeffrey B. Donnelly, John J. Parker, Suezanne E. Rhodes, Gary H. Felgner, Philip L. Dwarki, V. J. Gromkowski, Stanislaw H. Deck, R. Randall DeWitt, Corrille M. Friedman, Arthur Hawe, Linda A. Leander, Karen R. Martinez, Douglas Perry, Helen C. Shiver, John W. Montgomery, Donna L. Liu, Margaret A. |
| description | Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus infection. To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleoprotein was injected into the quadriceps of BALB/c mice. This resulted in the generation of nucleoprotein-specific CTLs and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival. |
| doi_str_mv | 10.1126/science.8456302 |
| format | Article |
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J. ; Gromkowski, Stanislaw H. ; Deck, R. Randall ; DeWitt, Corrille M. ; Friedman, Arthur ; Hawe, Linda A. ; Leander, Karen R. ; Martinez, Douglas ; Perry, Helen C. ; Shiver, John W. ; Montgomery, Donna L. ; Liu, Margaret A.</creator><creatorcontrib>Ulmer, Jeffrey B. ; Donnelly, John J. ; Parker, Suezanne E. ; Rhodes, Gary H. ; Felgner, Philip L. ; Dwarki, V. J. ; Gromkowski, Stanislaw H. ; Deck, R. Randall ; DeWitt, Corrille M. ; Friedman, Arthur ; Hawe, Linda A. ; Leander, Karen R. ; Martinez, Douglas ; Perry, Helen C. ; Shiver, John W. ; Montgomery, Donna L. ; Liu, Margaret A.</creatorcontrib><description>Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus infection. To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleoprotein was injected into the quadriceps of BALB/c mice. This resulted in the generation of nucleoprotein-specific CTLs and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.8456302</identifier><identifier>PMID: 8456302</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Analysis ; Animals ; Antibodies ; Antibody-dependent cell cytotoxicity ; Base Sequence ; Biological and medical sciences ; DNA ; DNA, Viral - genetics ; DNA, Viral - therapeutic use ; Experimental viral diseases and models ; Gene Expression ; Genetic Vectors ; Histocompatibility Antigens Class I - immunology ; Immunization ; Infections ; Infectious diseases ; Influenza A virus ; Influenza A virus - genetics ; Influenza A virus - immunology ; Influenza A virus - isolation & purification ; Influenza research ; Lung - microbiology ; Lungs ; Lymphocytes ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Muscles - metabolism ; Nucleoproteins - genetics ; Nucleoproteins - immunology ; Orthomyxoviridae Infections - microbiology ; Orthomyxoviridae Infections - prevention & control ; Plasmids ; RNA-Binding Proteins ; Spleen cells ; T lymphocytes ; T-Lymphocytes, Cytotoxic - immunology ; Transfection ; Vaccines ; Viral antigens ; Viral Core Proteins - genetics ; Viral Core Proteins - immunology ; Viral diseases ; Viral proteins ; Viral Vaccines - genetics ; Viruses</subject><ispartof>Science (American Association for the Advancement of Science), 1993-03, Vol.259 (5102), p.1745-1749</ispartof><rights>Copyright 1993 American Association for the Advancement of Science</rights><rights>1993 INIST-CNRS</rights><rights>COPYRIGHT 1993 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1993 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c714t-fc01dae47d34a49aed1dafd91d1426f7c1d57e4559ea931cd56b9a6e569d36873</citedby><cites>FETCH-LOGICAL-c714t-fc01dae47d34a49aed1dafd91d1426f7c1d57e4559ea931cd56b9a6e569d36873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2881215$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2881215$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4672949$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8456302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ulmer, Jeffrey B.</creatorcontrib><creatorcontrib>Donnelly, John J.</creatorcontrib><creatorcontrib>Parker, Suezanne E.</creatorcontrib><creatorcontrib>Rhodes, Gary H.</creatorcontrib><creatorcontrib>Felgner, Philip L.</creatorcontrib><creatorcontrib>Dwarki, V. J.</creatorcontrib><creatorcontrib>Gromkowski, Stanislaw H.</creatorcontrib><creatorcontrib>Deck, R. Randall</creatorcontrib><creatorcontrib>DeWitt, Corrille M.</creatorcontrib><creatorcontrib>Friedman, Arthur</creatorcontrib><creatorcontrib>Hawe, Linda A.</creatorcontrib><creatorcontrib>Leander, Karen R.</creatorcontrib><creatorcontrib>Martinez, Douglas</creatorcontrib><creatorcontrib>Perry, Helen C.</creatorcontrib><creatorcontrib>Shiver, John W.</creatorcontrib><creatorcontrib>Montgomery, Donna L.</creatorcontrib><creatorcontrib>Liu, Margaret A.</creatorcontrib><title>Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus infection. To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleoprotein was injected into the quadriceps of BALB/c mice. This resulted in the generation of nucleoprotein-specific CTLs and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival.</description><subject>Analysis</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibody-dependent cell cytotoxicity</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - therapeutic use</subject><subject>Experimental viral diseases and models</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Histocompatibility Antigens Class I - immunology</subject><subject>Immunization</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Influenza A virus</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - immunology</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza research</subject><subject>Lung - microbiology</subject><subject>Lungs</subject><subject>Lymphocytes</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Muscles - metabolism</subject><subject>Nucleoproteins - genetics</subject><subject>Nucleoproteins - immunology</subject><subject>Orthomyxoviridae Infections - microbiology</subject><subject>Orthomyxoviridae Infections - prevention & control</subject><subject>Plasmids</subject><subject>RNA-Binding Proteins</subject><subject>Spleen cells</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Transfection</subject><subject>Vaccines</subject><subject>Viral antigens</subject><subject>Viral Core Proteins - genetics</subject><subject>Viral Core Proteins - immunology</subject><subject>Viral diseases</subject><subject>Viral proteins</subject><subject>Viral Vaccines - genetics</subject><subject>Viruses</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0kGLEzEUB_BBlLWunr0ozEHEw85uMpkkk2Ota7dQtoLa65Amb4aUNFmTGXD99GbpUCkULDmE5P_Lg_Belr3F6Brjkt1EZcApuK4ryggqn2UTjAQtRInI82yCEGFFjTh9mb2KcYtQygS5yC5GPsnWd9BD8NZ3foj5t-B7UL3xLp920rjY5wvX2gHcH5lvHtNhO8a-zb_cT_Nbp7w2rstlvjZB2n0F415nL1ppI7wZ98vs59fbH7O7YrmaL2bTZaE4rvqiVQhrCRXXpJKVkKDTsdUCa1yVrOUKa8qholSAFAQrTdlGSAaUCU1Yzcll9nFf9yH4XwPEvtmZqMBa6SB9qOGUVZzQ8r8QM4q5KEWCV3vYSQuNca3vg1QdOEjf8w5ak66nmFBeYs4SL07wtDTsjDrlPx35RHr43XdyiLFZfL8_m67WZ9PP83NpPV8e0atTVHlroYMmNXK2OuI3e66CjzFA2zwEs5PhscGoeRrWZhzWZpy-9OL92JRhswN98P_yD2Muo5K2DdIpEw-sYrwU1VPL3u3ZNvY-HOKyrnGJKfkLvrz3fw</recordid><startdate>19930319</startdate><enddate>19930319</enddate><creator>Ulmer, Jeffrey B.</creator><creator>Donnelly, John J.</creator><creator>Parker, Suezanne E.</creator><creator>Rhodes, Gary H.</creator><creator>Felgner, Philip L.</creator><creator>Dwarki, V. 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J.</au><au>Gromkowski, Stanislaw H.</au><au>Deck, R. Randall</au><au>DeWitt, Corrille M.</au><au>Friedman, Arthur</au><au>Hawe, Linda A.</au><au>Leander, Karen R.</au><au>Martinez, Douglas</au><au>Perry, Helen C.</au><au>Shiver, John W.</au><au>Montgomery, Donna L.</au><au>Liu, Margaret A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1993-03-19</date><risdate>1993</risdate><volume>259</volume><issue>5102</issue><spage>1745</spage><epage>1749</epage><pages>1745-1749</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus infection. To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleoprotein was injected into the quadriceps of BALB/c mice. This resulted in the generation of nucleoprotein-specific CTLs and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>8456302</pmid><doi>10.1126/science.8456302</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1993-03, Vol.259 (5102), p.1745-1749 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75647352 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Analysis Animals Antibodies Antibody-dependent cell cytotoxicity Base Sequence Biological and medical sciences DNA DNA, Viral - genetics DNA, Viral - therapeutic use Experimental viral diseases and models Gene Expression Genetic Vectors Histocompatibility Antigens Class I - immunology Immunization Infections Infectious diseases Influenza A virus Influenza A virus - genetics Influenza A virus - immunology Influenza A virus - isolation & purification Influenza research Lung - microbiology Lungs Lymphocytes Medical sciences Mice Mice, Inbred BALB C Molecular Sequence Data Muscles - metabolism Nucleoproteins - genetics Nucleoproteins - immunology Orthomyxoviridae Infections - microbiology Orthomyxoviridae Infections - prevention & control Plasmids RNA-Binding Proteins Spleen cells T lymphocytes T-Lymphocytes, Cytotoxic - immunology Transfection Vaccines Viral antigens Viral Core Proteins - genetics Viral Core Proteins - immunology Viral diseases Viral proteins Viral Vaccines - genetics Viruses |
| title | Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein |
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