Solubilization of muscarinic acetylcholine receptors from mammalian brain: An analytical approach
Muscarinic receptor sites were extracted from microsomal membranes of mammalian brain using digitonin. These detergent preparations were submitted to the control of unambiguous solubilization criteria, and were found to correspond to molecularly dispersed material. In contrast, the corresponding sal...
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| Veröffentlicht in: | Analytical biochemistry 1981-02, Vol.111 (1), p.33-41 |
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| container_title | Analytical biochemistry |
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| creator | Gorissen, Hugo Aerts, Gustaaf Ilien, Brigitte Laduron, Pierre |
| description | Muscarinic receptor sites were extracted from microsomal membranes of mammalian brain using digitonin. These detergent preparations were submitted to the control of unambiguous solubilization criteria, and were found to correspond to molecularly dispersed material. In contrast, the corresponding salt preparations did not fulfill these criteria. After comparing various assay methods, we describe a simple and rapid charcoal adsorption binding technique, which was selected for measuring digitonin-extracted muscarinic receptors. Under these conditions the digitonin preparations, from four different species revealed identical biochemical properties in a stereospecific labeling system using [
3H]dexetimide and [
3H]levetimide. |
| doi_str_mv | 10.1016/0003-2697(81)90224-4 |
| format | Article |
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3H]dexetimide and [
3H]levetimide.</description><subject>Animals</subject><subject>Brain Chemistry</subject><subject>Cats</subject><subject>Cattle</subject><subject>Centrifugation, Density Gradient</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Chromatography, Gel</subject><subject>Dexetimide</subject><subject>Digitonin</subject><subject>Dogs</subject><subject>Female</subject><subject>Male</subject><subject>Rats</subject><subject>Receptors, Cholinergic - isolation & purification</subject><subject>Receptors, Muscarinic - isolation & purification</subject><subject>Solubility</subject><subject>Species Specificity</subject><subject>Stereoisomerism</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtq3DAUhkVJSCfTvkELWoVk4VaSZcvqIhBCLoVAFm3X4lg-IiqyNJXswOTp4-kMWWZ1Fv-N8xHyhbNvnPH2O2OsrkSr1XnHLzQTQlbyA1lxptuK1UwfkdWb5SM5LeUvY5zLpj0hJ0rUjai7FYFfKcy9D_4FJp8iTY6Oc7GQffSWgsVpG-xTCj4izWhxM6VcqMtppCOMIwQPkfYZfPxBryKFCGE7eQuBwmaTE9inT-TYQSj4-XDX5M_tze_r--rh8e7n9dVDZetGTdXgnNAMZMtQajso2QH0tlYCXaeEVGi5E53AweleSac7ybrFNwiH2FjU9Zqc7XuX2X8zlsmMvlgMASKmuRjVtLKVC5A1kXujzamUjM5ssh8hbw1nZkfW7LCZHTbTcfOfrJFL7Ouhf-5HHN5CB5SLfrnXcXny2WM2xXqMFge_gJvMkPz7A6-0aYo3</recordid><startdate>198102</startdate><enddate>198102</enddate><creator>Gorissen, Hugo</creator><creator>Aerts, Gustaaf</creator><creator>Ilien, Brigitte</creator><creator>Laduron, Pierre</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198102</creationdate><title>Solubilization of muscarinic acetylcholine receptors from mammalian brain: An analytical approach</title><author>Gorissen, Hugo ; Aerts, Gustaaf ; Ilien, Brigitte ; Laduron, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-dff290a460e49cd748aabc372ef87247ec1f282edf9b74f984089cdd2fee5ce93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Brain Chemistry</topic><topic>Cats</topic><topic>Cattle</topic><topic>Centrifugation, Density Gradient</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Chromatography, Gel</topic><topic>Dexetimide</topic><topic>Digitonin</topic><topic>Dogs</topic><topic>Female</topic><topic>Male</topic><topic>Rats</topic><topic>Receptors, Cholinergic - isolation & purification</topic><topic>Receptors, Muscarinic - isolation & purification</topic><topic>Solubility</topic><topic>Species Specificity</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gorissen, Hugo</creatorcontrib><creatorcontrib>Aerts, Gustaaf</creatorcontrib><creatorcontrib>Ilien, Brigitte</creatorcontrib><creatorcontrib>Laduron, Pierre</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gorissen, Hugo</au><au>Aerts, Gustaaf</au><au>Ilien, Brigitte</au><au>Laduron, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Solubilization of muscarinic acetylcholine receptors from mammalian brain: An analytical approach</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1981-02</date><risdate>1981</risdate><volume>111</volume><issue>1</issue><spage>33</spage><epage>41</epage><pages>33-41</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Muscarinic receptor sites were extracted from microsomal membranes of mammalian brain using digitonin. These detergent preparations were submitted to the control of unambiguous solubilization criteria, and were found to correspond to molecularly dispersed material. In contrast, the corresponding salt preparations did not fulfill these criteria. After comparing various assay methods, we describe a simple and rapid charcoal adsorption binding technique, which was selected for measuring digitonin-extracted muscarinic receptors. Under these conditions the digitonin preparations, from four different species revealed identical biochemical properties in a stereospecific labeling system using [
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| ispartof | Analytical biochemistry, 1981-02, Vol.111 (1), p.33-41 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Brain Chemistry Cats Cattle Centrifugation, Density Gradient Chemical Phenomena Chemistry Chromatography, Gel Dexetimide Digitonin Dogs Female Male Rats Receptors, Cholinergic - isolation & purification Receptors, Muscarinic - isolation & purification Solubility Species Specificity Stereoisomerism |
| title | Solubilization of muscarinic acetylcholine receptors from mammalian brain: An analytical approach |
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