Immunological recognition of NPS-gastrin derivatives by gastrin antibodies
NPS-gastrin (o-nitrophenylsulfenylgastrin) derivatives are potent inhibitors of stimulated gastric acid secretion in the rat. The aim of this work is to study the recognition by a gastrin antibody of several gastrin derivatives either with or without the addition of NPS to the C-terminal tryptophan...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1981-03, Vol.99 (2), p.511-515 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Accary, J.P. Laigneau, J.P. Pham Van Chuong, P. Morgat, J.L. Dubrasquet, M. |
| description | NPS-gastrin (o-nitrophenylsulfenylgastrin) derivatives are potent inhibitors of stimulated gastric acid secretion in the rat. The aim of this work is to study the recognition by a gastrin antibody of several gastrin derivatives either with or without the addition of NPS to the C-terminal tryptophan residue. We studied the following peptides and their NPS-derivatives: human gastrin I (2–17) (G), BOC-βAla-Trp-Met-Asp-PheNH
2 (pentagastrin) (Pg), its analog BOC-Gly-Trp-NLeu-Asp-PheNH
2 (NLeu-Pg)
2, t-AOC-Trp-Met-Asp-PheNH
2 9tetragastrin). In addition, (Dehydro(αβ)Trp)-pentagastrin (dehydro-Pg) was also tested. In a standard immunoassay system containing constant amounts of
125I-gastrin and gastrin antibody we measured the
B
F
ratio with varying amounts of gastrin and the above peptides. In the case of all penta and tetrapeptide derivatives the presence of the NPS radical promoted better recognition of the molecules by gastrin antibody. On the other hand the rigidity of the side chain of the tryptophan in the dehydro-Pg analog of pentagastrin drastically decreased the affinity of gastrin antibody for the molecule (
D 50 =
1
1000
that of pentagastrin). |
| doi_str_mv | 10.1016/0006-291X(81)91774-5 |
| format | Article |
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2 (pentagastrin) (Pg), its analog BOC-Gly-Trp-NLeu-Asp-PheNH
2 (NLeu-Pg)
2, t-AOC-Trp-Met-Asp-PheNH
2 9tetragastrin). In addition, (Dehydro(αβ)Trp)-pentagastrin (dehydro-Pg) was also tested. In a standard immunoassay system containing constant amounts of
125I-gastrin and gastrin antibody we measured the
B
F
ratio with varying amounts of gastrin and the above peptides. In the case of all penta and tetrapeptide derivatives the presence of the NPS radical promoted better recognition of the molecules by gastrin antibody. On the other hand the rigidity of the side chain of the tryptophan in the dehydro-Pg analog of pentagastrin drastically decreased the affinity of gastrin antibody for the molecule (
D 50 =
1
1000
that of pentagastrin).</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(81)91774-5</identifier><identifier>PMID: 7236278</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antibodies ; Antigen-Antibody Complex ; Biological Assay ; Gastric Juice - drug effects ; Gastrins - analysis ; Immunoassay ; Male ; Nitrobenzenes - pharmacology ; Radioimmunoassay ; Rats ; Structure-Activity Relationship</subject><ispartof>Biochemical and biophysical research communications, 1981-03, Vol.99 (2), p.511-515</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-1c4137144da49123a089ef1134a30700989f2913fee53466e8b76a539568a5db3</citedby><cites>FETCH-LOGICAL-c357t-1c4137144da49123a089ef1134a30700989f2913fee53466e8b76a539568a5db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X81917745$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7236278$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Accary, J.P.</creatorcontrib><creatorcontrib>Laigneau, J.P.</creatorcontrib><creatorcontrib>Pham Van Chuong, P.</creatorcontrib><creatorcontrib>Morgat, J.L.</creatorcontrib><creatorcontrib>Dubrasquet, M.</creatorcontrib><title>Immunological recognition of NPS-gastrin derivatives by gastrin antibodies</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>NPS-gastrin (o-nitrophenylsulfenylgastrin) derivatives are potent inhibitors of stimulated gastric acid secretion in the rat. The aim of this work is to study the recognition by a gastrin antibody of several gastrin derivatives either with or without the addition of NPS to the C-terminal tryptophan residue. We studied the following peptides and their NPS-derivatives: human gastrin I (2–17) (G), BOC-βAla-Trp-Met-Asp-PheNH
2 (pentagastrin) (Pg), its analog BOC-Gly-Trp-NLeu-Asp-PheNH
2 (NLeu-Pg)
2, t-AOC-Trp-Met-Asp-PheNH
2 9tetragastrin). In addition, (Dehydro(αβ)Trp)-pentagastrin (dehydro-Pg) was also tested. In a standard immunoassay system containing constant amounts of
125I-gastrin and gastrin antibody we measured the
B
F
ratio with varying amounts of gastrin and the above peptides. In the case of all penta and tetrapeptide derivatives the presence of the NPS radical promoted better recognition of the molecules by gastrin antibody. On the other hand the rigidity of the side chain of the tryptophan in the dehydro-Pg analog of pentagastrin drastically decreased the affinity of gastrin antibody for the molecule (
D 50 =
1
1000
that of pentagastrin).</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigen-Antibody Complex</subject><subject>Biological Assay</subject><subject>Gastric Juice - drug effects</subject><subject>Gastrins - analysis</subject><subject>Immunoassay</subject><subject>Male</subject><subject>Nitrobenzenes - pharmacology</subject><subject>Radioimmunoassay</subject><subject>Rats</subject><subject>Structure-Activity Relationship</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9Lw0AQxRdRaq1-A4WcRA_RnexmN3sRpPinUlRQwduySSZlJcnW3bTQb29qa4-eBmbeezPzI-QU6BVQENeUUhEnCj4vMrhUICWP0z0yBKponADl-2S4kxySoxC-KAXgQg3IQCZMJDIbkqdJ0yxaV7uZLUwdeSzcrLWddW3kquj59S2emdB520Ylers0nV1iiPJV9Nc2bWdzV1oMx-SgMnXAk20dkY_7u_fxYzx9eZiMb6dxwVLZxVBwYBI4Lw1XkDBDM4UVAOOGUUmpylTVn8wqxJRxITDLpTApU6nITFrmbETON7lz774XGDrd2FBgXZsW3SJomQreJ8teyDfCwrsQPFZ67m1j_EoD1WuEes1Hr_noDPQvQp32trNt_iJvsNyZtsz6-c1mjv2TS4teh8JiW2Bpe3ydLp39f8EPiDp_pw</recordid><startdate>19810331</startdate><enddate>19810331</enddate><creator>Accary, J.P.</creator><creator>Laigneau, J.P.</creator><creator>Pham Van Chuong, P.</creator><creator>Morgat, J.L.</creator><creator>Dubrasquet, M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810331</creationdate><title>Immunological recognition of NPS-gastrin derivatives by gastrin antibodies</title><author>Accary, J.P. ; Laigneau, J.P. ; Pham Van Chuong, P. ; Morgat, J.L. ; Dubrasquet, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-1c4137144da49123a089ef1134a30700989f2913fee53466e8b76a539568a5db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antigen-Antibody Complex</topic><topic>Biological Assay</topic><topic>Gastric Juice - drug effects</topic><topic>Gastrins - analysis</topic><topic>Immunoassay</topic><topic>Male</topic><topic>Nitrobenzenes - pharmacology</topic><topic>Radioimmunoassay</topic><topic>Rats</topic><topic>Structure-Activity Relationship</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Accary, J.P.</creatorcontrib><creatorcontrib>Laigneau, J.P.</creatorcontrib><creatorcontrib>Pham Van Chuong, P.</creatorcontrib><creatorcontrib>Morgat, J.L.</creatorcontrib><creatorcontrib>Dubrasquet, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Accary, J.P.</au><au>Laigneau, J.P.</au><au>Pham Van Chuong, P.</au><au>Morgat, J.L.</au><au>Dubrasquet, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunological recognition of NPS-gastrin derivatives by gastrin antibodies</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1981-03-31</date><risdate>1981</risdate><volume>99</volume><issue>2</issue><spage>511</spage><epage>515</epage><pages>511-515</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>NPS-gastrin (o-nitrophenylsulfenylgastrin) derivatives are potent inhibitors of stimulated gastric acid secretion in the rat. The aim of this work is to study the recognition by a gastrin antibody of several gastrin derivatives either with or without the addition of NPS to the C-terminal tryptophan residue. We studied the following peptides and their NPS-derivatives: human gastrin I (2–17) (G), BOC-βAla-Trp-Met-Asp-PheNH
2 (pentagastrin) (Pg), its analog BOC-Gly-Trp-NLeu-Asp-PheNH
2 (NLeu-Pg)
2, t-AOC-Trp-Met-Asp-PheNH
2 9tetragastrin). In addition, (Dehydro(αβ)Trp)-pentagastrin (dehydro-Pg) was also tested. In a standard immunoassay system containing constant amounts of
125I-gastrin and gastrin antibody we measured the
B
F
ratio with varying amounts of gastrin and the above peptides. In the case of all penta and tetrapeptide derivatives the presence of the NPS radical promoted better recognition of the molecules by gastrin antibody. On the other hand the rigidity of the side chain of the tryptophan in the dehydro-Pg analog of pentagastrin drastically decreased the affinity of gastrin antibody for the molecule (
D 50 =
1
1000
that of pentagastrin).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7236278</pmid><doi>10.1016/0006-291X(81)91774-5</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1981-03, Vol.99 (2), p.511-515 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies Antigen-Antibody Complex Biological Assay Gastric Juice - drug effects Gastrins - analysis Immunoassay Male Nitrobenzenes - pharmacology Radioimmunoassay Rats Structure-Activity Relationship |
| title | Immunological recognition of NPS-gastrin derivatives by gastrin antibodies |
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