Insulin-stimulated translocation of glucose transport systems in the isolated rat adipose cell. Time course, reversal, insulin concentration dependency, and relationship to glucose transport activity
A specific D-glucose-inhibitable ( super(3)H)cytochalasin B-binding assay is used to examine the time course, reversibility, and hormone concentration dependency of an insulin-induced translocation of glucose transport systems from a large intracellular pool, associated with a low density subfractio...
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| Veröffentlicht in: | The Journal of biological chemistry 1981-01, Vol.256 (10), p.4772-4777 |
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| container_end_page | 4777 |
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| container_issue | 10 |
| container_start_page | 4772 |
| container_title | The Journal of biological chemistry |
| container_volume | 256 |
| creator | Karnieli, E Zarnowski, M J Hissin, P J Simpson, I A Salans, L B Cushman, S W |
| description | A specific D-glucose-inhibitable ( super(3)H)cytochalasin B-binding assay is used to examine the time course, reversibility, and hormone concentration dependency of an insulin-induced translocation of glucose transport systems from a large intracellular pool, associated with a low density subfraction of the microsomal membranes, to the plasma membrane in isolated rat adipose cells. The number of glucose transport systems in the plasma membrane and glucose transport activity in the intact cell are compared during the time course and reversal of insulin's action. The results demonstrate that insulin increases the number of glucose transport systems in the plasma membrane of the isolated rat adipose cell through a rapid and reversible translocation of glucose transport systems from a specific intracellular membrane pool. |
| doi_str_mv | 10.1016/S0021-9258(19)69319-1 |
| format | Article |
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The number of glucose transport systems in the plasma membrane and glucose transport activity in the intact cell are compared during the time course and reversal of insulin's action. 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The results demonstrate that insulin increases the number of glucose transport systems in the plasma membrane of the isolated rat adipose cell through a rapid and reversible translocation of glucose transport systems from a specific intracellular membrane pool.</description><subject>adipocytes</subject><subject>Adipose Tissue - drug effects</subject><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological Transport, Active - drug effects</subject><subject>Cytochalasin B - metabolism</subject><subject>Glucose - metabolism</subject><subject>insulin</subject><subject>Insulin - pharmacology</subject><subject>Insulin Antibodies</subject><subject>Kinetics</subject><subject>Male</subject><subject>monosaccharide translocator</subject><subject>Rats</subject><subject>translocation</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtrFTEUzkKptfoTClmJwp2axzyXUqoWCi6s65CbnLGRTDLmZAr3F_ZvmZl76apgNgnne53wEXLJ2RVnvP38kzHBq0E0_Uc-fGoHyYeKvyLnz-M35C3iH1ZOPfAzctYxXjdNd06ebgMu3oUKs5sWrzNYmpMO6KPR2cVA40h_-8VEhCMwx5QpHjDDhNQFmh-AOoxHadKZauvmlW3A-yt676byjEtC2NEEj5BQ-10RbrEFCQZCMd6yLMwQLARz2FEdih34DcAHN9McX1hEm-weXT68I69H7RHen-4L8uvrzf319-rux7fb6y93lZG9yBXshRZ7LYwcta77xpquttYaK_vatnroQba6E62xokzaAQpd1nLf83GUptXygnw4-s4p_l0As5ocrj_VAeKCqmvamtWC_5fIm4Z1He8KsTkSTYqICUY1JzfpdFCcqbVdtbWr1hoVH9TWrloDLk8By34C-6w6VSv_AQd_qbc</recordid><startdate>19810101</startdate><enddate>19810101</enddate><creator>Karnieli, E</creator><creator>Zarnowski, M J</creator><creator>Hissin, P J</creator><creator>Simpson, I A</creator><creator>Salans, L B</creator><creator>Cushman, S W</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19810101</creationdate><title>Insulin-stimulated translocation of glucose transport systems in the isolated rat adipose cell. 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| ispartof | The Journal of biological chemistry, 1981-01, Vol.256 (10), p.4772-4777 |
| issn | 0021-9258 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | adipocytes Adipose Tissue - drug effects Adipose Tissue - metabolism Animals Binding Sites Biological Transport, Active - drug effects Cytochalasin B - metabolism Glucose - metabolism insulin Insulin - pharmacology Insulin Antibodies Kinetics Male monosaccharide translocator Rats translocation |
| title | Insulin-stimulated translocation of glucose transport systems in the isolated rat adipose cell. Time course, reversal, insulin concentration dependency, and relationship to glucose transport activity |
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